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Disinfectant Testing Laboratory

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Disinfectant Efficacy Testing

Proper surface disinfection is a key factor in preventing the spread of infections from contaminated surfaces. Surface disinfection plays a crucial role in high-risk areas such as hospital settings and public spaces. Healthcare facilities and public health administrators rely on disinfectants and antiseptics to maintain hygiene. Disinfectants eliminate pathogenic microorganisms from inanimate objects, while antiseptics are used on living tissues to kill or prevent microbial growth. Therefore, it is crucial to test these products for efficacy and safety before they are marketed.

Evaluation of product efficacy

International standards such as EN (European Standards), ISO (International Organization for Standardization), ASTM (American Society for Testing and Materials), and AOAC (Association of Official Agricultural Chemists) provide guidelines for assessing the bactericidal, fungicidal/yeasticidal, and virucidal efficacy of chemical disinfectants and antiseptics. Manufacturers are advised to choose the test standards based on regulations set by relevant regulatory authorities, as these requirements may vary by region. Evaluation criteria depend on the intended use of the product and the test conditions specified in standard guidelines.

Disinfectant Products Tested

Disinfectant and antiseptic formulations used across various sectors such as human medicine, food handling and processing, industrial, domestic, veterinary settings, and institutional settings are tested at MIS.

We receive various product samples for testing such as –

  • Hygienic handrub
  • Handwash
  • Instrument disinfectants
  • Surface disinfectants
  • Textile disinfectants

Disinfectant and Sanitizer Test Methods performed

Microbe Investigations offers a comprehensive range of efficacy testing for surface disinfectants and antiseptics, including antibacterial, antiviral, and fungicidal/yeasticidal tests. These standards include European standards (EN), ISO standards, ASTM standards, and AOAC standards.

Antibacterial Test Methods For Disinfectants

EN Standards:

  • EN 1040: 2005 – Quantitative (phase 1 suspension) test to evaluate the basic bactericidal activity of chemical disinfectants and antiseptics
  • EN 14347: 2005 – Quantitative (phase 1 suspension) test for basic sporicidal activity of chemical disinfectants and antiseptics
  • EN 13727: 2012 + A2:2015 – Quantitative (phase 2 step 1 suspension) test for bactericidal activity of chemical disinfectants intended for medical area
  • EN 14348: 2005 – Quantitative (phase 2 step 1 suspension) test for mycobactericidal or tuberculocidal efficacy testing of chemical disinfectants and antiseptics intended for medical area
  • EN 17126: 2018 – Quantitative (phase 2 step 1 suspension) test for sporicidal efficacy testing of chemical disinfectants and antiseptics intended for medical area
  • EN 1656: 2019 – Quantitative (phase 2 step 1 suspension) test for bactericidal efficacy testing of chemical disinfectants and antiseptics intended for veterinary area
  • EN 14204: 2012 – Quantitative (phase 2 step 1 suspension) test for mycobactericidal efficacy testing of chemical disinfectants and antiseptics intended for veterinary area
  • EN 13623: 2020 – Quantitative (phase 2 step 1 suspension) test for bactericidal activity of disinfectants against Legionella pneumophila in aqueous systems
  • EN 13704: 2018 – Quantitative (phase 2 step 1 suspension) test for sporicidal efficacy testing of chemical disinfectants used in food, industrial, domestic, and institutional areas
  • EN 1499: 2013 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of hygienic hand wash products used in the medical area
  • EN 1500: 2013 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of hand rub products used in the medical area
  • EN 12791: 2016 + A1: 2017 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of chemical disinfectants and antiseptics intended for surgical hand disinfection
  • EN 14561: 2006 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of chemical disinfectants and antiseptics intended for instrument disinfection in medical areas
  • EN 14563: 2008 – Quantitative (phase 2 step 2 suspension) test for mycobactericidal or tuberculocidal efficacy testing of chemical disinfectants intended for instrument disinfection in the medical area
  • EN 16615: 2015 – Quantitative (phase 2 step 2 suspension) test for assessing bactericidal and yeasticidal activity of disinfectant wipes used in medical areas
  • EN 16616: 2015 – Quantitative (phase 2 step 2 suspension) test for chemical-thermal textile disinfection used in media areas
  • EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
  • EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in medical areas
  • EN 14349: 2012 – Quantitative (phase 2 step 2 suspension) test for Evaluation of the bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area
  • EN 16437: 2014 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area
  • EN 13697: 2015 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic, and institutional areas

ASTM Standards:

  • ASTM E2799: 20212 – Determination of disinfectant efficacy against Pseudomonas aeruginosa biofilm using the MBEC Assay
  • ASTM E2871: 2021 – Determination of disinfectant efficacy against biofilm grown in the CDC biofilm reactor using the single-tube method
  • ASTM E2274: 2016 – Determination of microbicidal efficacy of laundry sanitizers and disinfectants
  • ASTM E2406: 2016 – Evaluation of bactericidal efficacy of laundry sanitizers and disinfectants for use in high-efficiency washing operations
  • ASTM E2362: 2022 – Evaluation of antimicrobial efficacy of pre-saturated or impregnated towelettes for hard surface disinfection
  • ASTM E1054: 2022 – Test method for evaluation of inactivators of antimicrobial agents
  • ASTM E1153: 2022 – Evaluating the efficacy of sanitizers intended for inanimate, hard, non-porous, non-food contact surfaces
  • ASTM E1174: 2021 – Test method for healthcare personnel handwash formulations
  • ASTM E2276: 2019 – Test method for bactericidal activity of hygienic handwash and hand rub agents

AOAC Standards:

  • AOAC 955.14 – AOAC Use Dilution Method for disinfectants against Salmonella enterica
  • AOAC 955.15 – AOAC Use Dilution Method for disinfectants against Staphylococcus aureus
  • AOAC 964.02 – AOAC Use Dilution Method for disinfectants against Pseudomonas aeruginosa
  • AOAC 955.16 – Chlorine equivalency method for sanitizers and disinfectant products
  • AOAC 960.06 – Determination of germicidal and detergent sanitizing action of disinfectants
  • AOAC 965.12 – Evaluation of the tuberculocidal activity of disinfectants
  • AOAC 965.13 – Test method for swimming pool water disinfectants
  • AOAC 966.04 – Test method for evaluating sporicidal activity of disinfectants
  • AOAC 991.47 – Evaluating the bactericidal activity of disinfectants against Salmonella choleraesuis

EPA Standards:

  • EPA MLB SOP MB 40 – Evaluating the efficacy of antimicrobial surface coatings
  • EPA #01 01A – Determining residual sanitizing efficacy of dried chemical residues on hard non-porous surface
  • EPA MLB SOP MB 41 – Determining antimicrobial activity of hard, non-porous copper-containing surface products

PAS Standards:

  • PAS 2424 – Evaluating residual bactericidal and/or yeasticidal) efficacy of liquid chemical disinfectants

Antiviral Test Methods For Disinfectants

EN Standards:

  • EN 17914: 2022 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of chemical disinfectants and antiseptics in food, industrial, domestic, and institutional areas
  • EN 17915: 2022 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of chemical disinfectants on hard non-porous surfaces in food, industrial, domestic, and institutional areas
  • EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
  • EN 14476: 2013 + A2: 2019 – Quantitative (phase 2 step 1 suspension) test for the virucidal activity of disinfectant or antiseptics used in medical areas
  • EN 14675: 2015 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of disinfectant or antiseptics used in veterinary areas
  • EN 13610: 2002 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of disinfectant against bacteriophages used in food, industrial, and domestic areas
  • EN 16777: 2018 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of disinfectant used in the medical area
  • EN 17111: 2018 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of medical instrument disinfectants
  • EN 17122: 2019 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of chemical disinfectants or antiseptics used in veterinary areas

ASTM Standards:

  • ASTM E1053: 2020 – Assessing virucidal activity of chemicals used for disinfection of inanimate, non-porous environmental surfaces
  • ASTM E1838: 2017 – Evaluating virus eliminating efficacy of hygienic handwash and handrub Agent

Fungicidal/Yeasticidal Test Methods For Disinfectants

  • EN 1275: 2005 – Quantitative (phase 1 suspension) test for evaluating basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
  • EN 13624: 2013 – Quantitative (phase 2 step 1 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
  • EN 1657: 2016 – Quantitative (phase 2 step 1 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for veterinary area
  • EN 1650: 2019 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
  • EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants and antiseptics intended for medical areas
  • EN 16438: 2014 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for veterinary area
  • EN 14562: 2006 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity for instruments disinfectants used in the medical area
  • EN 13697: 2015 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for bactericidal and/or fungicidal activity of chemical disinfectants and antiseptics intended for food, industrial, domestic, and institutional areas
  • EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
  • EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in medical areas

PAS Standards

  • PAS 2424 – Test method for evaluating residual bactericidal and/or yeasticidal efficacy of liquid chemical disinfectants

To learn more about disinfectant testing, visit this page – The Ultimate Guide to disinfectant testing

Test microorganisms used for antimicrobial testing at MIS

Bacterial strains

  • Staphylococcus aureus (ATCC 6538P)
  • Klebsiella pneumoniae (ATCC 4352)
  • Escherichia coli (ATCC 8739)
  • Legionella spp. (ATCC 700511)
  • Salmonella enterica (ATCC 10708)
  • Pseudomonas aeruginosa (ATCC 15442)
  • Enterococcus hirae (ATCC 10541)
  • MRSA (ATCC 3391)

Viral strains

  • Human adenoviruses (ATCC VR-5)
  • Vaccinia virus (ATCC VR-1549)
  • Betacoronavirus 1 (ATCC VR-1558)
  • Human coronavirus (ATCC VR-740)
  • Influenza A virus (H3N2) (ATCC VR-1679)
  • Influenza A virus (H1N1) (ATCC VR-1469)
  • Feline calicivirus (FCV) F-9 (US Patent strain)
  • Poliovirus Type 1 (Sabin) (LSc-2ab)
  • Murine norovirus (MNV) (S99)

Fungal and yeast strains

  • Aspergillus niger (ATCC 16888)
  • Penicillium funiculosum (ATCC 11797)
  • Chaetomium globosum (ATCC 6205)
  • Aureobasidium pullulans (ATCC 15233)
  • Candida auris (MYA5002)
  • Candida albicans (MTCC-3017)

Why Disinfectant Testing is Important?

Ensuring proper surface disinfection is a key factor in preventing cross-infections resulting from contact with contaminated surfaces. Disinfectants and antiseptics are extensively used to maintain hygiene and prevent the spread of pathogens. Disinfectants are effective in eliminating pathogenic microbes such as bacteria, viruses, and fungi that exist on inanimate objects. Similarly, antiseptics are used to kill or prevent the growth of pathogens on living tissues such as skin.

To ensure the safety and effectiveness of disinfectants and antiseptic products, it is critical to conduct a thorough evaluation to determine their efficacy against dangerous microorganisms. This type of testing can help ensure that these products meet the necessary standards and are suitable for use in real-world conditions.

Why trust MIS with your Disinfectant Product Testing?

At MIS, we utilize advanced technologies to evaluate the effectiveness of disinfectants and antiseptics. Our team of expert microbiologists meticulously oversees testing processes to ensure precision and accuracy. We specialize in designing and executing both qualitative and quantitative tests, tailored to meet the specific needs of our clients. Our commitment at MIS is to fully understand your testing requirements and assess your products against all essential quality criteria within a set timeframe. For comprehensive disinfectant efficacy testing of your products, contact us today.

FAQs

The efficacy of a disinfectant is tested using standardized laboratory methods. These methods typically involve exposing contaminated surfaces to a known concentration of disinfectant for a specific contact time and measuring the reduction in the number of viable microorganisms.

The length of exposure, or contact time, is critical because insufficient contact time may not effectively kill or inactivate all microorganisms on the surface, reducing the overall efficacy of the disinfectant.

The label on the product or the manufacturer’s instructions will indicate what pathogens the disinfectants will be effective against and the required contact time for effective disinfection.

Disinfectants are used to kill or inactivate microorganisms on inanimate surfaces, while antiseptics are applied to living tissue to inhibit microbial growth and reduce the possibility of infection​.

Disinfectants should be tested periodically, often annually, or whenever there are changes in formulation, application procedures, or regulations​.

Yes, disinfectant testing can be customized to meet specific application needs, including testing against particular microorganisms or under specific environmental conditions.

Commonly tested disinfectants include surface disinfectants, instrument disinfectants, textile disinfectants, hygienic handwashes, and hand rubs​.

Key parameters evaluated during disinfectant testing include microbial reduction and testing conditions like contact time, concentration, temperature, and the presence of organic matter.

Get your product tested now

✓  Completed test order form (TOS)
✓  Customization and Assistance to determine the right test for your project
✓  FAST TRACK Program with less Turnaround Time

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