Affordable
Global Footprint
Speed
LIMS system
Disinfectant Testing Laboratory
Disinfectant Efficacy Testing
Disinfectant efficacy testing plays a crucial role in ensuring that disinfectant products and formulations effectively eliminate harmful microorganisms. The evaluation criteria of product efficacy depend upon the intended use of the product and the test conditions stipulated in standard guidelines.
Various international standards are available to test the efficacy of disinfectants, such as EN (European standards), ISO (International Organization for Standardization), ASTM (American Society for Testing and Materials), and AOAC (Association of Official Agricultural Chemists). These standards are vital in evaluating the bactericidal, virucidal, and fungicidal properties of products and ensure that they meet regulatory requirements. It is recommended to choose test standards based on the geographical location and the regulations set forth by the relevant authorities.
Disinfectant and Sanitizer Test Methods performed at MIS
Microbe Investigations offers a comprehensive range of antibacterial, antifungal, and antiviral efficacy tests for surface disinfectants and antiseptic formulations. Our team of experts is dedicated to providing accurate and reliable results to ensure the safety and effectiveness of these products.
Antibacterial Test Methods For Disinfectants
- EN 1040: 2005 – Quantitative (phase 1 suspension) test to evaluate the basic bactericidal activity of chemical disinfectants and antiseptics
- EN 14347: 2005 – Quantitative (phase 1 suspension) test for basic sporicidal activity of chemical disinfectants and antiseptics
- EN 13727: 2012 + A2:2015 – Quantitative (phase 2 step 1 suspension) test for bactericidal activity of chemical disinfectants intended for medical area
- EN 14348: 2005 – Quantitative (phase 2 step 1 suspension) test for Mycobactericidal or tuberculocidal efficacy testing of chemical disinfectants and antiseptics intended for medical area
- EN 17126: 2018 – Quantitative (phase 2 step 1 suspension) test for Sporicidal efficacy testing of chemical disinfectants and antiseptics intended for medical area
- EN 1656: 2019 – Quantitative (phase 2 step 1 suspension) test for Bactericidal efficacy testing of chemical disinfectants and antiseptics intended for veterinary area
- EN 14204: 2012 – Quantitative (phase 2 step 1 suspension) test for Mycobactericidal efficacy testing of chemical disinfectants and antiseptics intended for veterinary area
- EN 13623: 2020 – Quantitative (phase 2 step 1 suspension) test for bactericidal activity of disinfectants against Legionella pneumophila in aqueous systems
- EN 13704: 2018 – Quantitative (phase 2 step 1 suspension) test for Sporicidal efficacy testing of chemical disinfectants used in food, industrial, domestic, and institutional areas
- EN 1499: 2013 – Quantitative (phase 2 step 2 suspension) test for Bactericidal efficacy testing of hygienic hand wash products used in the medical area
- EN 1500: 2013 – Quantitative (phase 2 step 2 suspension) test for Bactericidal efficacy testing of hand rub products used in the medical area
- EN 12791: 2016 + A1: 2017 – Quantitative (phase 2 step 2 suspension) test for Bactericidal efficacy testing of chemical disinfectants and antiseptics intended for surgical hand disinfection
- EN 14561: 2006 – Quantitative (phase 2 step 2 suspension) test for Bactericidal efficacy testing of chemical disinfectants and antiseptics intended for instrument disinfection in medical areas
- EN 14563: 2008 – Quantitative (phase 2 step 2 suspension) test for Mycobactericidal or tuberculocidal efficacy testing of chemical disinfectants intended for instrument disinfection in the medical area
- EN 16615: 2015 – Quantitative (phase 2 step 2 suspension) test for Assessing bactericidal and yeasticidal activity of disinfectant wipes used in medical areas
- EN 16616: 2015 – Quantitative (phase 2 step 2 suspension) test for chemical-thermal textile disinfection used in media areas
- EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
- EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in medical areas
- EN 14349: 2012 – Quantitative (phase 2 step 2 suspension) test for Evaluation of the bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area
- EN 16437: 2014 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area
- EN 13697: 2015 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic, and institutional areas
- ASTM E2799: 20212 – Determination of disinfectant efficacy against Pseudomonas aeruginosa biofilm using the MBEC Assay
- ASTM E2871: 2021 – Determination of disinfectant efficacy against biofilm grown in the CDC biofilm reactor using the single-tube method
- ASTM E2274: 2016 – Determination of microbicidal efficacy of laundry sanitizers and disinfectants
- EN 14204:2012 – Quantitative (phase 2 step 1 suspension) test to evaluate the mycobactericidal activity of disinfectant intended for Veterinary area
- ASTM E2406: 2016 – Evaluation of bactericidal efficacy of laundry sanitizers and disinfectants for use in high-efficiency washing operations
- ASTM E2362: 2022 – Evaluation of antimicrobial efficacy of pre-saturated or impregnated towelettes for hard surface disinfection
- ASTM E1054: 2022 – Test method for evaluation of inactivators of antimicrobial agents
- ASTM E1153: 2022 – Evaluating the efficacy of sanitizers intended for inanimate, hard, non-porous, non-food contact surfaces
- ASTM E1174:2 021 – Test method for healthcare personnel handwash formulations
- ASTM E2276: 2019 – Test method for bactericidal activity of hygienic handwash and hand rub Agents
- ASTM E2315: 2023 – Determination of antimicrobial activity using a time-kill procedure
- AOAC 955.14 – AOAC Use Dilution Method for disinfectants against Salmonella enterica
- AOAC 955.15 – AOAC Use Dilution Method for disinfectants against Staphylococcus aureus
- AOAC 964.02 – AOAC Use Dilution Method for disinfectants against Pseudomonas aeruginosa
- AOAC 955.16 – Chlorine equivalency method for sanitizers and disinfectant products
- AOAC 960.06 – Determination of germicidal and detergent sanitizing action of disinfectants
- AOAC 965.12 – Evaluation of Tuberculocidal activity of disinfectants
- AOAC 965.13 – Test method for swimming pool water disinfectants
- AOAC 966.04 – Test method for evaluating sporicidal activity of disinfectants
- AOAC 991.47 – Evaluating of bactericidal activity of disinfectants against Salmonella choleraesuis
- EN 17658: 2022 – Test method for chemical textile disinfectants intended for domestic area
- EN 17422: 2022 – Evaluating the efficacy of teat disinfectants intended for veterinary area
- EN 1276: 2010 – Quantitative suspension test to evaluate the bactericidal activity of chemical disinfectants and antiseptics
- PAS 2424 – Evaluating residual bactericidal and/or yeasticidal) efficacy of liquid chemical disinfectants
- EPA MLB SOP MB 40 – Evaluating the efficacy of antimicrobial surface coatings
- EPA #01 01A – Determining residual sanitizing efficacy of dried chemical residues on hard non – porous surface
- EPA MLB SOP MB 41 – Determining antimicrobial activity of hard, non-porous copper-containing surface products
Antiviral Test Methods For Disinfectants
- EN 14476: 2013 + A2: 2019 – Quantitative (phase 2 step 1 suspension) test for f virucidal activity of disinfectant or antiseptics used in medical areas
- EN 14675: 2015 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of disinfectant or antiseptics used in veterinary areas
- EN 13610: 2002 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of disinfectant against bacteriophages used in food, industrial, and domestic areas
- EN 16777: 2018 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of disinfectant used in the medical area
- EN 17111: 2018 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of medical instrument disinfectants
- EN 17122: 2019 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of chemical disinfectants or antiseptics used in veterinary areas
- ASTM E1053: 2020 – Assessing virucidal activity of chemicals used for disinfection of inanimate, non-porous environmental surfaces
- ASTM E1838: 2017 – Evaluating virus eliminating efficacy of hygienic handwash and handrub Agent
- EN 17914: 2022 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of chemical disinfectants and antiseptics in food, industrial, domestic, and institutional areas
- EN 17915: 2022 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of chemical disinfectants on hard non-porous surfaces in food, industrial, domestic, and institutional areas
- EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
Fungicidal/Yeasticidal Test Methods For Disinfectants
- EN 1275: 2005 – Quantitative (phase 1 suspension) test for evaluating basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 13624: 2013 – Quantitative (phase 2 step 1 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 1657: 2016 – Quantitative (phase 2 step 1 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for veterinary area
- BS EN 1650: 2019 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 16438: 2014 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for veterinary area
- EN 14562: 2006 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity for instruments disinfectants used in the medical area
- EN 13697: 2015 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for bactericidal and/or fungicidal activity of chemical disinfectants and antiseptics intended for food, industrial, domestic, and institutional areas
- EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
- EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in medical areas
- PAS 2424 – Test method for evaluating residual bactericidal and/or yeasticidal efficacy of liquid chemical disinfectants
To learn more about disinfectant testing, visit this page – Ultimate guide to disinfectant testing
Why Disinfectant Testing is Important?
Ensuring proper surface disinfection is a key factor in preventing cross-infections resulting from contact with contaminated surfaces. Disinfectants and antiseptics are extensively used to maintain hygiene and prevent the spread of pathogens. Disinfectants are effective in eliminating pathogenic microbes such as bacteria, viruses, and fungi that exist on inanimate objects. Similarly, antiseptics are used to kill or prevent the growth of pathogens on living tissues such as skin.
To ensure the safety and effectiveness of disinfectants and antiseptic products, it is critical to conduct a thorough evaluation to determine their efficacy against dangerous microorganisms. This type of testing can help ensure that these products meet the necessary standards and are suitable for use in real-world conditions.
Disinfectant Products Tested at MIS
At MIS, we assess the efficacy of chemical disinfectant and antiseptic formulations utilized across diverse sectors, including human medicine, veterinary settings, food handling and processing, industrial, domestic, and institutional settings.
We receive various product samples for testing such as –
- Hygienic handrub
- Handwash
- Instrument disinfectants
- Surface disinfectants
- Textile disinfectants
Test microorganisms used for antimicrobial testing at MIS
Bacterial strains
- Staphylococcus aureus (ATCC 6538P)
- Klebsiella pneumoniae (ATCC 4352)
- Escherichia coli (ATCC 8739)
- Legionella spp. (ATCC 700511)
- Salmonella enterica (ATCC 10708)
- Pseudomonas aeruginosa (ATCC 15442)
- Enterococcus hirae (ATCC 10541)
- MRSA (ATCC 3391)
Viral strains
- Human adenoviruses (ATCC VR-5)
- Vaccinia virus (ATCC VR-1549)
- Betacoronavirus 1 (ATCC VR-1558)
- Human coronavirus (ATCC VR-740)
- Influenza A virus (H3N2) (ATCC VR-1679)
- Influenza A virus (H1N1) (ATCC VR-1469)
- Feline calicivirus (FCV) F-9 (US Patent strain)
- Poliovirus Type 1 (Sabin) (LSc-2ab)
- Murine norovirus (MNV) (S99)
Fungal and yeast strains
- Aspergillus niger (ATCC 16888)
- Penicillium funiculosum (ATCC 11797)
- Chaetomium globosum (ATCC 6205)
- Aureobasidium pullulans (ATCC 15233)
- Candida auris (MYA5002)
- Candida albicans (MTCC-3017)
Why trust MIS with your Disinfectant Product Testing?
At MIS, we employ state-of-the-art technologies to assess the effectiveness of disinfectants and antiseptics. Our highly skilled microbiologists closely monitor testing analysis to guarantee precise and accurate results.
We adeptly design and execute both qualitative and quantitative tests to assess the efficacy of disinfectants, thereby, catering to the unique testing needs of our clients.
At MIS, we are committed to thoroughly understanding our customer’s testing requirements and evaluating their products against all crucial quality criteria within a defined time frame.
For disinfectant efficacy testing of your products, reach us here now.
Get your product tested now
✓ Completed test order form (TOS)
✓ Customization and Assistance to determine the right test for your project
✓ FAST TRACK Program with less Turnaround Time
