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Determination of Algal Resistance of Plastic Films

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ASTM  G29 – Determination of Algal Resistance of Plastic Films

The ASTM G29 test method is used to evaluate the efficacy of antimicrobial-treated surfaces against algae growth.

Algae require a moist or damp environment to thrive, hence are ubiquitous near or inside water bodies. Man-made water retaining structures such as swimming pools, irrigation ditches, water storage tanks, and artificial ponds are lined up with plastic films. Over time, algae proliferate to form large microfilms that can penetrate the structure and create unsightly deposits on this film. 

To avoid the widespread of algae in artificial water structure environments, antifungal coating or films are developed that can resist the growth of algae. The aim of  ASTM G29 test is to check the degree of surface protection from algae by using antimicrobial integrated plastic films. 

Organisms typically used in this method: Blue-green algae Oscillatoria sp. (cyanobacteria)

ASTM G29 Test Method

ASTM G29 Test Method

  1. Propagation tank – In the test method, the propagation tank is used to provide the optimal conditions for the growth of cyanobacteria (test inoculum). Cyanobacteria are propagated on both treated and untreated polymeric mesh screens under fluorescent lighting. 
  2. Treated and untreated samples are suspended in jars containing homogenized cyanobacteria.
  3. Jars are further kept for incubation in both darkness and light for 12 hrs.
  4. Every 3 – 4 days jars are reinoculated with homogenized cyanobacteria.
  5. After the completion of 14 days of the test, test samples (both treated and untreated) are separated and evaluated for fungal growth.

Major Advantages of ASTM G29

ASTM G29 is a simple and cost-effective test method for the determination of algal resistance of plastic films. This test procedure typically takes 14 days and the results of the test can be easily evaluated by visual inspection.

Microbe Investigations is a microbiology testing laboratory, run by research professionals and technical experts. Our experts are highly skilled in conducting microbiological analysis on product samples meanwhile following strict safety procedures to avoid contamination. 

For more information on antifungal testing of plastic films and our other testing services, get a free consultation from our expert today.


ASTM G29 test method determines the efficacy of  plastic films integrated with anti-algae additives to resist the growth of surface-growing algae

ASTM G29 test is applicable for polymeric films used in man-made water structure environments such as swimming pools, irrigation ditches, water storage tanks, and artificial ponds.

ASTM G29 testing duration is 14 days to 30 days (incubation time of test materials after inoculation) based on the type of sample and material tested.

At Microbe Investigations, we test for the ASTM G29 using the Blue-green algae Oscillatoria sp.

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