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ISO 22196 Test

Measure Antibacterial activity on Plastics Surfaces

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ISO 22196 : 2007 – Measurement of Antibacterial activity on Plastics Surfaces

Bacteria can survive on hard surfaces such as plastics and other non-porous materials for several days and continue to be pathogenic. Thereby, the aim of the development of antibacterial solid materials is to mitigate the disease-borne microbes for ensuring the safety of public health.

The ISO 22196  is designed to evaluate the efficacy of antibacterial treated plastic and other non-porous materials in preventing microbial contamination. ISO 22196 is a harmonized version of JIS Z 2801 that is almost similar to JIS Z 2801.

Sample Preparation

  • Test samples –  Requires at least 3 specimens from the treated test material.
  • Control sample – Requires 6 specimens of the untreated material. The three untreated samples determine the viable count immediately after the inoculation. The remaining three samples are used to know the viable count after inoculation for 24 hrs.

Note – The size of both treated and untreated sample materials should not be more than 10 mm in thickness. All the test specimens are kept separately in sterile petri plates to avoid cross-contamination. 

Test Process of ISO 22196

ISO 22196 Test Procedure

  • Test bacterial inoculum is placed on the testing samples (treated and untreated) and is covered with a piece of film (size of 40 mm × 40 mm) to prevent the spread of the inoculum from the edges.
  • These petri plates are incubated for 24 hrs under specific temperature (35°C) and humidity conditions.
  • For untreated samples (control) – 10 ml of SCDLP broth (used as a neutralizer) is added to petri plates containing sample material immediately after incubation. Further, 10-fold serial dilution is performed with the recovered neutralizing solution to enumerate the viable bacteria. TSA (Tryptic Soya Agar) is used as a bacterial growth medium and plates are incubated at 35 °C for 40 to 48 hours.
  • For treated samples (test) –  10 ml of SCDLP broth (used as a neutralizer) is added to petri plates having samples after the incubation of 24 hours. Further, 10-fold serial dilution is performed and is plated on  Plate Count Agar (PCA) to enumerate the viable bacteria . Plates are incubated at 35 °C for 40 h to 48 hours.
  • After incubation, CFU (Colony Forming Unit) is counted for plates (sample and control) containing 30 – 300 colonies.
  • Bactericidal activity of the testing material is determined by evaluation of reduction in  viable bacterial count of treated samples as compared to the control samples.

We are a leading, global test service provider in the field of microbiology, specializing in testing for textiles, plastics, coatings, and surface disinfectants. With a team of highly experienced microbiology professionals and well-equipped laboratory facilities, we aim to provide maximum efficacy and accuracy at an affordable price.

For more information on the ISO 22196 test you can consult our experts. Our team of highly qualified professionals is always there to understand your needs and deliver the best outcomes.


ISO 22196 is a quantitative test used to determine the antimicrobial activity of antibacterial treated plastic materials to kill bacteria (bactericidal) or inhibit their growth (bacteriostatic).

ISO 22196 testing (antimicrobial surface test) is applicable for plastics materials and other non-porous materials such as steel and glass.

ISO 22196 test method takes 3 to 4 days.

At Microbe Investigations, we test for the ISO 22196 using the following bacterial strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica ( ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on customer’s request.

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