Measures the Resistance of Pathogenic Bacteria to Antibacterial agents
Zone of Inhibition test (also referred to as Kirby-Bauer test) is used to determine the susceptibility or resistance of pathogenic bacteria to antibacterial agents.
In comparison to other laboratory test methods, the zone of Inhibition test is an inexpensive and fast way to determine antibacterial activity.
Major advantage is this test can be used for a variety of antibacterial product samples such as treated textiles, polymers, disinfectants, and antibiotics.
Based on the observations, it is concluded that growth media can also hinder the growth of microorganisms instead of antimicrobial agents.
Minimum Inhibitory Concentration (MIC) – It is the lowest concentration of antimicrobial agent that prevents the visible growth of microorganisms after incubation. The Minimum Inhibitory Concentration test determines the antimicrobial activity of a test agent against a specific bacteria.
Zone of Inhibition (ZOI) – It is a clear circular area around antimicrobial discs in which bacteria are unable to grow.
Both these terms are inversely proportional to each other. It depends on the susceptibility of the test organism to the antimicrobial agent. Higher the susceptibility, larger the zone of inhibition and lower the MIC. Whereas, a higher MIC value and a small zone of inhibition indicate that bacteria are resistant to antimicrobial agents.
When it comes to evaluating the antimicrobial activity of treated products, quantitative tests are found quite useful as they produce more accurate and clear results.
We offer a complete spectrum of antimicrobial testing services for all major industries (Textiles, Plastics, Coatings, and Surface disinfectants) developing antimicrobial products. In our well-equipped laboratories, we determine the antimicrobial effectiveness of products using international standards such as ISO, JIS, AATCC, and ASTM.
To get a quote and a free expert consultation, contact here. With a customer-centric approach, our clear focus is on ensuring on-time delivery with accuracy.
Zone of Inhibition test is a qualitative test method used to measure the antibacterial efficacy of products formulated to inhibit the growth of harmful bacteria.
Zone of Inhibition test is applicable to check the antibacterial efficacy of treated textiles, polymers, disinfectants, and antibiotics.
The Zone of Inhibition test takes 3-4 days to complete.
At Microbe Investigations, we test for Zone of Inhibition using the following bacteria: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica ( ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.