Swiss Anti-Bacterial & Anti-Viral Testing Laboratory, ISO Certified | MIS

EN 1276 Test Method

Quantitative suspension test for the evaluation of bactericidal activity of disinfectants or antiseptics

Chemical disinfectants and antiseptics

Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas

1. The test method involves a sample of product added to a test suspension of bacteria in a solution of an interfering substance (Clean Condition: 0.3g/l and Dirty condition: 3g/l of Bovine Serum Albumin).

2. The mixture is maintained at the chosen test temperature for the adopted contact time.

3. At the end of this contact time, an aliquot is taken and neutralized immediately.

4. Following a 5-minute neutralization period, 1 ml is plated onto Tryptone Soya Agar to detect surviving test bacteria.

Organisms typically used in this method: Staphylococcus aureus (ATCC 6538P), Pseudomonas aeruginosa (ATCC 15442), Enterococcus hirae (ATCC 10541) and Escherichia coli (ATCC 8739)

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