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ASTM D3273

Antifungal Resistance Test for Coatings

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ASTM D3273 : 2021 – Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber

ASTM D3273 is a qualitative method to check the performance of coatings that are intended to prevent growth of mold/fungus on the interior environments.

ASTM D3273 test is performed in an environmental chamber to test the effect of “real environmental conditions” on product performance. Test employs moisture and temperature conditions that normally support mold growth.

ASTM D3273 Test Conditions & Requirements 

Environmental chamber – Capable to maintain a relative humidity of 95 to 98% at 32.5 +/- 1°C.

Mandatory test microorganisms – Aureobasidium pullulans (ATCC 9348), Aspergillus niger (ATCC 6275) and Penicillium (ATCC 9849)

Incubation period/Contact time 4 weeks

ASTM D3273 Test Method

  1. Greenhouse soil is placed in the tray within the chamber and  water is added to the tank chamber to desired depth. The environmental growth chamber is equilibrated including that of soil and water contents for a 24 hour period at approx 32°C.
  2. Mold suspensions from Aureobasidium, Aspergillus niger, and Penicillium are evenly spread over the surface of the greenhouse soil in the tray in the cabinet. Mold is allowed to sporulate for a period of two weeks before testing.
  3. Treated and untreated product samples are exposed to the chamber by vertical hanging for 4 weeks.
  4. Products samples are examined for mold growth once every week for a period of 28 days.
  5. Mold growth is rated on a scale of 0-10, which indicates –
  • 0 – Full mold growth
  • 10 – No growth (no visual defacement)

Importance of ASTM D3273 Test

  • Defacement of interior environments is often related to uncontrolled fungal or mold growth. Mold resistant or antifungal coatings are designed to restore shelf life and aesthetic value of interior surfaces. These antifungal coatings are formulated using biocide agents that combat or inhibit the proliferation of mold and fungus on surfaces.
  • ASTM (American Society for Testing and Materials) specifies standard methods for paints and coating industries to determine efficacy of materials for their intended applications.
  • ASTM D3273 antifungal test provides all test parameters and passing criteria to evaluate performance of treated coatings for use on interior surfaces.

At MIS, we are committed to develop customized and analytical solutions to support quality development of antifungal coating materials. With extensive experience in antifungal testing, our experts design and execute ASTM D3273 testing protocols that are well suited for product chemistry.

At customers’s request, we also perform ASTM G21Antifungal Resistance Test for polymeric materials.

Would you like to receive a quote for ASTM D3273 or have other questions and needs, talk to our experts now.


ASTM D3273 test is a qualitative antifungal test to assess the resistance of interior coatings against mold and fungus growth. This 28 days test is conducted in an environmental chamber stimulating real environmental conditions to evaluate the antifungal performance of sample materials.

ASTM D3273 test is applicable for antifungal coatings designed for use in interior environments.

ASTM D3273 test takes 6-7 weeks to complete.

At Microbe Investigations, we test for ASTM D3273 using the following fungal strains : Aspergillus niger (ATCC-168880), Aureobasidium, and Penicillium. Additional strains can be added as per the customer’s testing requirements.

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