Evaluation of Bactericidal and Yeasticidal Activity of Disinfectant Wipes used in the Medical Area
EN 16615 test, also known as 4 field test, is a quantitative test method for determining the bactericidal and yeasticidal activity of hospital-grade disinfectant wipes. EN 16615 method is applicable for wipes intended for disinfection of non-porous surfaces used in health care settings, in the clinics of kindergartens or schools, dental clinic setups, and in domestic areas.
In hospital environments, inanimate surfaces are one of the major sources of pathogenic microorganisms along with muti-drug-resistant strains. Thereby, disinfection practices play an essential role in preventing pathogen surface colonization and cross-contamination in healthcare settings. However, researchers have concluded that despite the use of disinfectants, there is a possibility of cross-infection among patients. Because not all disinfectant products hold strong antimicrobial potential against potent pathogens.
Prior to EN 16615, standard methods used to check the efficacy of disinfectant products were not able to clarify the mechanical removal of test organisms in real conditions. This recently developed EN 16615 standard thoroughly assesses the efficacy of disinfectant wipes including bactericidal and yeasticidal activity, and mechanical removal of microbial contamination.
In order to make a working culture for EN 16615 test method, subcultures are prepared from the original culture. For bactericidal activity, 2nd and 3rd subculture can be used for the experiment. Whereas, in the case of yeast, 2nd subculture (48 hrs old) is used for the test procedure.
At MIS, we help our customers in identifying the antimicrobial effectiveness of their products. While prioritizing customers’ convenience, we are constantly focused on accuracy and on-time delivery of services.
With a specialized team of experts, Microbe Investigations (MIS) offers a full range of microbiology testing services for disinfectants, textiles, coating, and plastics. Our labs are equipped with world-class equipment and facilities which help to mitigate the chances of errors in all the steps of the antimicrobial evaluation process.
We use EN 16615 and EN 1276 standard protocols for the antibacterial analysis of surface disinfectants.
To get a quote for EN 16615 or any related queries contact us here.
EN 16615 test or 4 field test is the standard protocol that specifies the test procedure for determining the bactericidal and yeasticidal activity of disinfectant wipes intended to be used in hospitals.
EN 16615 tests can be applied for surface disinfectant products including disinfectant wipes, surgical hand rubs, and instrument disinfectants used in health care settings.
EN 16615 test takes 3-4 days to complete.
At Microbe Investigations, we test for the EN 16615 using the following bacterial and fungal strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017).