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Minimum Bactericidal Concentration

Determines Minimum Concentration of Antimicrobial agent that has Bactericidal (killing bacteria) Properties

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Minimum Bactericidal Concentration (MBC) Test

The Minimum Bactericidal Concentration (MBC) test determines the minimum concentration of antibacterial agents which kill a particular bacteria in a given time period. In antibacterial testing, MBC test can be done after the determination of Minimum Inhibitory Concentration (MIC).

Minimum Bactericidal Concentration test is used to evaluate the efficacy of new drugs and several other antimicrobial agents on a range of pathogenic bacteria.

MBC test is a relatively inexpensive antimicrobial susceptibility test that can be used to check the potency of multiple antimicrobial agents.

Advantages of Minimum Bactericidal Concentration Test

  • It is used to evaluate multiple antimicrobial agents for potency.
  • Minimum Bactericidal Concentration test can also be utilized to address the challenges of antimicrobial formulations wherein the active ingredient is being “bound up” by other ingredients. Hence, MBC values are very poor for the formulations in which active ingredients are chemically combined with other ingredients, hence can’t have bactericidal effects.
  • MBC test yields accurate and reliable results.

Minimum Bactericidal Concentration (MBC) Test Method

  • Overnight grown culture of test microorganisms is diluted in growth-supporting broth (Mueller Hinton Broth) between 1 x 10^5 and 1 x 10^6 cfu/ml.
  • Serial dilutions of antimicrobial test compound are made.
  • All the dilutions of test compound are inoculated with an equal volume of test microorganisms.
  • These dilutions are further transferred into 96-well Microtiter Plates. In parallel, a growth control (bacterial suspension without any antimicrobial agent) and sterile control (media without bacteria where no bacterial growth is observed after incubation) are also used in the test procedure. 
  • Microtiter plate is further incubated at a specific temperature and duration.
  • After incubation bacterial growth is observed,  turbidity indicates the growth of the microorganism, and lowest concentration of dilution with no turbidity or visible growth is referred as MIC (Minimum Inhibitory Concentration).
  • 2-3 dilutions showing MIC are plated on a suitable growth medium and incubated to determine the viable cells.
  • The lowest concentration that shows 99.9% reduction of the tested bacteria is MBC.

At Microbe Investigations, we offer a comprehensive array of antimicrobial (antibacterial & antiviral) testing services. Also, we have been a long-term expert in the field of entomology testing services.

With a team of highly experienced microbiologists and advanced testing facilities, Microbe Investigations is always betrothed in assisting our clients with the most reliable and accurate testing services.

We firmly believe in timely delivery and cost-effective testing services to balance efficiency, quality, and surpasses customer expectations. For MBC testing of your products or any other related test queries, reach out our experts here.

FAQs

Minimum Bactericidal Concentration (MBC) test is used to determine minimum concentration of antimicrobial agent that has bactericidal (killing bacteria) properties.

Minimum Bactericidal Concentration test is applicable for drugs, antiseptics, disinfectants, and other liquid formulations.

Minimum Bactericidal Concentration test takes 3-4 days.

At Microbe Investigations, we test for MBC using the following bacteria: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica ( ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.

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