1. Contact times are selected so that the test material would be effective in significantly reducing microbial counts during usage.

2. This test is performed with treated and non-treated samples of the test material. In accordance with AATCC 100 test procedure, 1 ml of test organism suspension at about 1000,000 CFU/ml is inoculated to the test sample.

3. At a given time, organisms are extracted with the help of a diluted and plated neutralizer medium.

4. This procedure tests both bacteriostatic activity (inhibition of multiplication) and bactericidal activity (killing of bacteria) of the antimicrobial test agent. The hydrophobic characteristic of fabrics can affect the antimicrobial activity test results obtained by the AATCC 100 test method.