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BS EN 14349: 2012
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Quick understanding of the test
BS EN 14349: 2012 - Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area on non-porous surfaces without mechanical action (phase 2, step 2)
EN 14349 is a Phase 2, Step 2 carrier test that evaluates the bactericidal efficacy of surface disinfectants and antiseptics used in veterinary settings on non-porous surfaces.
Application
- Enterococcus hirae
- Proteus vulgaris
- Pseudomonas aeruginosa
- Staphylococcus aureus
- Bacterial suspension and interfering substance are inoculated on the metal disc surface (carrier) and dried.
- The disinfectant sample is applied to the carrier and allowed to interact for a specified contact time.
- After contact time, the carrier is transferred to a neutralizing solution.
- The neutralizing solution is plated and further incubated to determine the remaining viable bacterial cells.
- Ensures that the disinfectants used in veterinary settings are effective at reducing bacterial loads.
- Provides a clear and consistent method for testing bactericidal activity, ensuring reproducibility and comparability of results.
Turnaround Time
Passing criteria
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Abstract
The European standard EN 14349 is designed to evaluate the bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area on non-porous surfaces without mechanical action. This phase 2, step 2 test simulates practical conditions without mechanical action to determine a productโs effectiveness in real-world veterinary use scenarios.
Scope of Application
EN 14349 applies to chemical disinfectants and antiseptics used in veterinary settings, such as
- Animal breeding,
- Transport,
- Husbandry,
- Production,
- and disposal of all animals (excluding when in food chain following death and entry to the processing industry)
EN 14349 Test Conditions & Requirements
EN 14349 Test Method
- Bacterial inoculation and interfering substance are spread onto the metal disc surface.
- On the dried inoculated surface, disinfectant sample is added and allowed to interact for a specified contact period time. In parallel, a test is performed for control where water is used instead of disinfectant sample.
- At the end of the contact period, metal disc is transferred to the neutralizing solution to stop the bactericidal activity.
- A portion of neutralizing solution is plated and incubated for 2 days to determine remaining viable bacterial cells.
- The number of bacterial colonies found on test samples are compared with control.
Passing criteria
A product must demonstrate at least โฅ 4 log reduction to pass the test.
Importance of EN 14349 Test
Non-porous surfaces in veterinary settings are the most common reservoir of pathogens, despite being easy to disinfect. Therefore, products used for surface disinfectants should be capable of killing or inactivating many or the most common pathogenic microorganisms.
As a Phase 2, Step 2 test, EN 14349 simulates practical, real-world usage conditions including variables like organic soiling, temperature, and contact time. This method provides a critical benchmark for manufacturers and regulators to determine whether a disinfectant is suitable for controlling microbial contamination in environments where animals are housed, transported, or treated.
Conclusion
At MIS, we design and perform antimicrobial efficacy tests for a wide range of disinfectant and antiseptic formulations. We have a team of microbiology professionals and technical experts who are continuously betrothed in understanding & analyzing testing requirements and creating test protocols for disinfectant testing.
Also, other available and most frequently performed tests in MIS labs are EN 1040 and EN 1276 for antibacterial disinfectant efficacy testing.
To get a quote on EN 14349 or any information related to our testing services, contact our experts now
Frequently Asked Questions
DR. Martinoz Scholtz
EN 14349 test is used to determine the bactericidal activity of surface disinfectants and antiseptics intended for use in veterinary areas.
EN 14349 is applicable to chemical disinfectants and antiseptic products used on non-porous surfaces in veterinary environments.
EN 14349 test takes 1-2 weeks to complete.
At Microbe Investigations Switzerland, we test for EN 14349 using the following bacterial and fungal strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Aspergillus niger (ATCC-16888), Candida albicans (MTCC 3017).
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