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Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method

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AATCC 147 – Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method

AATCC 147, also known as Parallel Streak Method is a qualitative test method to determine the bacteriostatic activity of diffusible antimicrobial finishes on textile materials. However, this test method is not applicable for textile materials that can’t be diffused through bacterial growth medium or are impregnated with any antibacterial-neutralizing substances.

AATCC 147 test method is easy to perform and provides reliable and reproducible results. Additionally, it is helpful for expediting the screening of a large amount of samples in comparison to other commonly used quantitative standard methods like AATCC 100 and JIS L 1902.

AATCC 147 Test Method

AATCC 147 Test Method

  • Molten agar media is poured into the sterile petri plates and allowed to solidify.
  • A sterile loop is used to obtain the inoculum from a 24-hour-old broth and is transferred to the surface of the agar medium. 
  • Through the edge of the inoculated area, 5 streaks are made on the agar medium.
  • Test samples (treated)  of size 25 × 50 mm are placed across the 5 streaks ensuring proper contact between the antimicrobial agents and test bacteria. Meanwhile, a parallel experiment is performed with control (untreated) samples. 
  • All petri plates are incubated at 37 ± 2°C for 18-24 h.
  • After incubation, petri plates are examined for interrupted bacterial growth along the streaks and clear zone of inhibition that occur due to the presence of the antibacterial agents. 

Microbe Investigation has an exclusive facility for antimicrobial testing, which caters to the requirement of clients worldwide. We help our clients to identify the efficacy of their antimicrobial products under specified test conditions such as temperature, test organisms, incubation period, etc.

For the antibacterial testing of textile materials, we offer both qualitative (AATCC-147) and quantitative test methods (AATCC 100, JIS L 1902 & ISO 20473). 

Are you looking for fast and accurate antimicrobial testing of your products? Consult our experts today and get a quote.


AATCC 147, also known as Parallel Streak Method is a qualitative test method specifying the bacteriostatic activity of diffusible antimicrobial agents on textile materials. 

AATCC 147 test method is applicable to all textile products (such as cloth, wadding, thread, bedclothes, and home furnishings) and other porous materials.

AATCC 147 test takes 3-4 days to complete.

At Microbe Investigations, we understand the need for accuracy and speed. We test for AATCC 147 standard using the following bacteria: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica ( ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.

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