Introduction

The Japanese Industrial Standards (JIS) L 1902 method has been developed to test the antibacterial activity and efficacy of fabrics treated with antimicrobial agents.

Test Components

Designed to test the antimicrobial activity of various fabrics, the JIS L 1902 method includes both qualitative and quantitative components.  

The qualitative component of the JIS L 1902 testing method is a qualitative determination of growth on a textile. The quantitative method necessitates an extra incubation period for the determination of the bacterial population by plating.

Methods of evaluation

JIS L 1902 is similar to standard antimicrobial textile test methods that examine textile materials following a brief incubation period. Ideally, this method is used for high throughput, accelerated turnaround, and substantial volume testing.

Absorption Method

In this method, the test bacteria is inoculated directly onto the sample. The standard contact time for the JIS L 1902 test method is 18 to 24 hours. The amount of bacteria present directly after inoculation and after the stipulated contact time is measured and used to estimate the antibacterial activity.

Transfer Method

This evaluation method consists of placing the test bacteria on an agar plate and subsequently transferring them onto the samples. The sample textile materials are kept in contact with the bacteria for five minutes and incubated for 18 to 24 hours. The amount of bacteria present directly after inoculation and after the stipulated contact time is measured and used to estimate the antibacterial activity.

Printing Method

In this evaluation method, the test bacteria are placed on a filter and printed onto the samples.

Halo Method

The textile sample is kept in contact with the surface of these inoculated agar plates. The plates are incubated for 24 to 48 hours and then assessed for “halos”. This is a qualitative method that estimates antibacterial activity through the appearance of halos [clear area of growth] around the sample.

The incubation stipulations of the JIS L 1902 test makes it one of the most rigorous methods for evaluating antimicrobial activity. This presents a robust replicate analysis and durability of the treated test sample.

The test protocol

The microbes to be tested are prepared by culturing them in a liquid culture medium. The test organisms are standardised through dilution in a nutritive broth. This broth offers optimal conditions for the microbes to grow. Optimal growth conditions are essential for the test to be conclusive. 

The control and test fabric samples are inoculated with the microbes in triplicate. These triplicates are incubated in closed containers at body temperature for 18 hours. The initial number of microbes are estimated at “time zero” through elution, dilution, and plating of the control textiles directly after inoculation. 

Post incubation, the final microbial concentrations are measured. The decrease in microorganisms relative to initial concentrations and the control fabric is estimated. The controls used are examined to guarantee that the elution method neutralises the antimicrobial agent in the test samples. 

jis l 1902 test method

Microbe Investigations

Founded in 2012, we are a spin-off company of the Swiss Federal Institute of Technology in Zürich. We offer specialised microbiological investigation services for the industry and aim at supporting the product development process of our partners in various sectors.

As a JIS L 1902 testing lab, our team works to estimate the antimicrobial activity of various textile products. Samples range from cloth, wadding, thread and material for clothing, home furnishings, and different goods. They can be tested independent of the type of antimicrobial agent used or the mode of application.

The JIS L 1902 testing in our lab takes two weeks. The booking process is uncomplicated and fast. We will do the JIS L 1902 testing at our lab and submit the report on your preferred date. The test can be performed for the following organisms Staphylococcus aureus (ATCC 6538P), Klebsiella pneumoniae (ATCC 4352), Escherichia coli (ATCC 8739), Candida albicans, Salmonella entercia, and Pseudomonas aeruginosa. Other organisms can be tested on request after verification.
For more information and to book a test, please reach out to us. We are happy to help!