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ISO 20743 Test

Determination of Antimicrobial Activity of Textile Clothes

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ISO 20743 Test Protocol – Determination of Antimicrobial Activity of Textile Clothes

ISO 20743 is a standard test method that determines the antimicrobial efficacy of antibacterial finished textiles.

New stylish and affordable clothing brands are hitting the market every day. Aside from enhancing the style statements, the textile industry has put efforts to innovate clothing materials that ensure safety from bacterial infections as well. Such clothing materials are named “antibacterial clothes” as they have the ability to kill or reduce harmful bacteria in contact with fabric material.

What actually necessitates the need for antibacterial clothes is, many pathogenic bacteria can survive on clothes for a good period of time. Since clothing is used everywhere, hence it becomes an easy mode of spreading bacterial infections. 

ISO 20743 and JIS L 1902 are the most commonly used test methods to determine the antimicrobial activity of textile clothes. These two methods are very identical technically but are named by two different organizations. JIS stands for Japanese Industrial Standard (JIS) and ISO stands for International Organization for Standardization. At MIS, we perform both of these test methods to check the efficacy of antibacterial treated clothes.

Products to be Tested with ISO 20743

ISO 20743 test method is applicable to all textile products regardless of the type of antibacterial agents used for method of application. It includes –

  • cloth, 
  • wadding, 
  • thread,
  • bedclothes, 
  • home furnishings,
  • and miscellaneous goods.

Different Methods of Testing in ISO 20743

Three different inoculation methods are available to determine the antimicrobial activity of the treated textiles. At MIS, our experts use “absorption method” to determine the antimicrobial activity of treated samples.

  1. Absorption method – In this method, test bacterial suspension is inoculated directly onto the treated antibacterial sample. 
  2. Transfer method – Transfer method involves the inoculation of agar plate with test bacteria and then transferring it onto the treated antibacterial sample. This sample is further incubated for 18-24 hours under humid conditions.
  3. Printing method – In this evaluation method, test bacteria are placed on a filter and printed onto the treated antibacterial sample. Sample is incubated for 1-4 hrs under humid conditions.
iso 20743 test method

ISO 20743 Test Procedure 

  • 6 test specimens of treated (antibacterial)  and 6 control samples of untreated (without antibacterial agents) fabric are needed.
  • All sample materials are subjected to sterilization to avoid any contamination.
  • Test bacterial suspension of a known titre is directly inoculated on the sample material.
  • For zero minute titre determination, (control and treated) samples are subjected to immediate wash with 20 ml of neutralizing media (SCDLP broth)  after bacterial inoculation. Other remaining six specimens (3 control and 3 testing samples)  are incubated at 37 °C ± 2 °C for 18 h to 24 h with the test bacterial suspension
  • After an incubation period of 18-24 h, the reaction is stopped by adding 20 ml of neutralizing media Soya Casein Digest Lecithin Polysorbate Broth (SCDLP) into tubes. Then, the tubes are shaken to ensure proper mixing.
  • Serial dilution and plating are done to determine titre of the remnant viable bacteria   post the contact time  
  • The decrease in microorganism growth is compared to the control fabric.

We, at Microbial Investigations, are constantly engaged in providing high-performance and quality testing services to our valuable customers. Meanwhile, we are equally focused on offering a superior customer experience by ensuring cost-effectiveness and timely delivery of services.

For placing a test request and more information on ISO 20743 test or any other related microbial testing services, reach us here

FAQs

ISO 20743 is a ‘quantitative test’ used to determine the antibacterial activity of all antibacterial textile products. It is designed to test the ability of fabrics that have been treated with antimicrobial agents to prevent microbial growth and to kill microorganisms. 

ISO 20743 test method is applicable to all textile products such as cloth, wadding, thread, bedclothes, home furnishings, and miscellaneous goods.

The ISO 20743 test takes 3 to 4 days to complete.

At Microbe Investigations, we understand the need for accuracy and speed. We test for the ISO 20743 using the following viruses: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica ( ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.

Contact us for more information

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