ISO 21702 Test
Measurement of Antiviral Activity on Plastics and
other Non-Porous Surfaces
This International Standard (ISO 21702) is a proper method for measuring antiviral activity on plastics and other non-porous surfaces of antiviral-treated products against viruses.
ISO 21702 is a unified test protocol that measures antiviral activity on non-porous and plastic surfaces, designed to provide protection against viruses.
Many epidemiological data and studies have confirmed that viruses can remain viable on a variety of porous and non-porous surfaces for several hours to a few days. In the case of latest COVID 19 outbreak, exposure to virus-infected surfaces is identified as a crucial risk factor for a high transmission rate of infection.
Hence, antiviral coating surfaces are innovated to prevent the spread of viral infection through inanimate surfaces. Standard protocols like ISO 21702 are used to evaluate the antiviral activity of non-porous surfaces incorporated with antiviral additives.
Whereas, ISO 22196 is a standard protocol used for the measurement of antibacterial activity on plastics and other non-porous surfaces
Products that can be tested with ISO 21702
The ISO 21702 test can be used to evaluate antiviral activity on plastics and other non-porous surfaces such as
- Metal substrates
- Natural and artificial leather, rubber, etc.
Test sample – 9 sterilized test (treated) specimens with a size of (50 ±2) mm X (50 ±2) mm, are required for ISO 21702 testing.
- 3 treated samples are used to determine the infectious titre of the virus after a contact period of 24 hrs.
- 6 treated samples are used as a control to know the suppressive efficiency of the test sample on the host. It means no cytotoxic effect on the host cell, no reduction of the cell sensitivity to the virus, and an inactivation of the antiviral activity in the neutralizing broth.
Control sample – 12 sterilized control (untreated) specimens with a size of (50 ±2) mm X (50 ±2) mm, are required for ISO 21702 testing.
- 3 untreated samples are needed to know the infectious titer of the virus immediately after inoculation and 3 untreated samples are used to measure the infectious titer of the virus after a contact period of 24 hrs.
- 6 untreated samples are used as a control test to confirm the suppressive efficiency of the test specimen on the host. It means no cytotoxic effect on the host cell, no reduction of the cell sensitivity to the virus, and an inactivation of the antiviral activity in the neutralizing broth.
ISO 21702 Test Method
- Control and treated samples are placed separately in sterile plates.
- 400 uL of test inoculum is spread over the surface of the samples.
- 40 mm X 40 mm cover slip is placed on the inoculum and pressed gently such that the inoculum spreads to the edges but does not leak outside the edges.
- Immediately after inoculation, 10 ml of either the SCDLP broth or other neutralizer is added to the petri dishes comprising 3 untreated test specimens.
- After a contact period of 24 hours, 10 ml of either the SCDLP broth or other neutralizer is added to treated and untreated samples to recover the remaining virus. Further, this neutralizing solution is serially diluted up to 10 dilutions, and the infectious titre of the recovered virus is determined either by Plaque assay or by TCID50 assay. Other assays can also be used based on the virus strain.
Antiviral Activity and Performance Standard
The antiviral activity is determined by the following equation.
R = Log10 (Ut) – Log10 (At)
R is the antiviral activity value
Log10 (Ut) is the logarithm average of 3 infectivity titre value after specific contact time with the control specimen
Log10 (At) is the logarithm average of 3 infectivity titre values after specific contact time with the test specimen.
The antiviral activity value is used to evaluate the performance of the plastics and other non-porous substrates. The values of antiviral activity representing good performance shall be agreed upon by all the interested parties.
At Microbe Investigation, we carry out ISO 21702 testing for a variety of plastic and non-porous surfaces including coating materials, metal substrates, ceramics, natural and artificial leather, rubber, etc.
Our experienced microbiologists and technical experts are at the forefront of quality assurance testing. While performing the microbiological assays, our experts implicate research, analytical, and interpretation skills to ensure that product’s antimicrobial efficacy and safety are accurately assessed under specified conditions.
For more information on our antimicrobial test capabilities and services, contact our experts today.
The ISO 21702 is a test method for measuring antiviral activity on plastics and other non-porous surfaces of antiviral-treated products against specified viruses.
The ISO 21702 can be used to evaluate antiviral activity on plastics and other non-porous surfaces such as coating materials, metal substrates, ceramics, natural and artificial leather, rubber, etc.
The turnaround time for ISO 21702 test is 4 weeks. We also offer “Fast Track” program, where the turnaround time is 2-3 weeks depending on the virus strain.
At Microbe Investigations, we understand the need for accuracy and speed. We test for the ISO 21702 using the following viruses: SARS CoV-2 (Omicron, Delta, Wuhan variant), Betacoronavirus 1 (OC43) (ATCC VR-1558), Human coronavirus 229E (ATCC VR-740), Influenza A (H1N1) (ATCC VR-1469), Influenza A (H3N2) (ATCC VR-1679), Human Poliovirus type 1 (LSc-2ab), Human Adenovirus type 5 (ATCC VR-5), Murine Norovirus (S99), Vaccinia virus (ATCC VR-1549), and Feline calicivirus (strain F-9).