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ISO 21702 Test

Measurement of Antiviral Activity on Plastics and
other Non-Porous Surfaces

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This International Standard (ISO 21702) is a proper method for measuring antiviral activity on plastics and other non-porous surfaces of antiviral-treated products against viruses.

ISO 21702 is a unified test protocol introduced in 2019, and it is used to evaluate the antiviral activity of plastics and other non-porous surfaces against specified viruses. The test protocol was written for Influenza A (H3N2, and H1N1), and Feline calicivirus, but can be used to evaluate antiviral efficacy against other viruses after successful validation.

Products that can be tested with ISO 21702

The ISO 21702 can be used to evaluate antiviral activity on plastics and other non-porous surfaces such as coating materials, metal substrates, ceramics, natural and artificial leather, rubber, etc.

ISO 21702 Test Method

Control Specimen: 12 sterilized (either steam-sterilized in Autoclave or dry sterilized in a hot oven or wiped with 70% Ethanol) control specimens with a size of (50 ±2) mm X (50 ±2) mm, are required for ISO 21702 testing. The following tests are performed on the Control Specimen.

• 6 control specimens are used for suppressive efficiency testing (no cytotoxic effect on the host cell, no reduction of the cell sensitivity to the virus, and inactivation of the antiviral activity in the neutralizing broth)

• 3 control specimens to determine the infectious titre of the remaining virus immediately after inoculation, and

• 3 control specimens to determine the infectious titre of the remaining virus after inoculation is in contact with the control specimen for a specific contact time (Standard contact time is 24 hours, but other contact time up to 24 hours is also applicable based on customer requirement)

Treated Specimen: 9 sterilized (either steam-sterilized in Autoclave or dry sterilized in a hot oven or wiped with 70% Ethanol) treated specimens with a size of (50 ±2) mm X (50 ±2) mm is required for ISO 21702 testing. The following tests are performed on the Treated Specimen.

• 6 treated specimens are used for suppressive efficiency testing (no cytotoxic effect on the host cell, no reduction of the cell sensitivity to the virus, and inactivation of the antiviral activity in the neutralizing broth), and

• 3 treated specimens to determine the infectious titre of the remaining virus after inoculum is in contact with the treated specimen for the same contact time as a control specimen

Infectious titre of recovered viruses: 400 uL of the virus is inoculated on both the control and the treated specimen. The virus from the control specimen is recovered immediately and after contact, time using a wash-out solution. Place the 40 mm X 40 mm cover slip on the inoculum and press gently such that the inoculum spreads to the edges but does not leak outside the edges. After the specified contact time, the virus from both the control and the treated specimen is recovered using a wash-out solution. The wash-out solution is serially diluted up to 10 dilutions, and the infectious titre of the recovered virus is determined either by Plaque assay or by TCID50 assay. Other assays can also be used based on the virus strain.

iso 21702 testing method

Antiviral Activity and Performance Standard:

The antiviral activity is determined by the following equation.

R = Log10 (Ut) – Log10 (At)

Where,

R is the antiviral activity value

Log10 (Ut) is the logarithm average of 3 infectivity titre value after specific contact time with the control specimen

Log10 (At) is the logarithm average of 3 infectivity titre value after specific contact time with the test specimen.

 The antiviral activity value is used to evaluate the performance of the plastics and other non-porous substrate. The values of antiviral activity representing the good performance shall be agreed upon by all the interested parties.

FAQs

The ISO 21702 can be used to evaluate antiviral activity on plastics and other non-porous surfaces such as coating materials, metal substrates, ceramics, natural and artificial leather, rubber, etc.

The turnaround time for ISO 21702 test is 4 weeks. We also offer “Fast Track” program, where the turnaround time is 2-3 weeks depending on the virus strain.

Contact us for more information

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