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Aerobic Plate Count

Measurement of Mesophilic Aerobic Bacteria in a given sample

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Aerobic Plate Count Test

The Aerobic Plate Count (APC) provides an estimation of mesophilic aerobic bacteria in a given sample. Mesophilic bacteria refers to a group of bacteria that can grow and thrive at moderate temperature between 20°C and 45°C.

It is also known as standard plate count, aerobic mesophilic count, total plate count, or aerobic colony count. Aerobic plate count method’s limitation is that it does not distinguish between different bacterial colonies on a given sample.

Importance of Aerobic Plate Count Test

  • Aerobic Plate Count test is primarily used for microbial analysis of food samples to avoid public health concerns caused by food contamination. The results of the test indicate the major hygiene issues, quality of raw material, storage conditions, and shelf life of the product or anticipated changes that occur due to enzyme degradation.
  • Aside from this, the Aerobic Plate Count Test method can also be used in the field of agriculture and to monitor the quality of drinking water.

Aerobic Plate Count Test Method

  • Food samples are first homogenized using a stomacher machine.
  • This homogenized solution is diluted 10 times (10-1 to 10-10) using the serial dilution technique.
  • 1 ml of each dilution is transferred to different petri dishes containing agar growth medium.
  • Plates are incubated at 35°C for 48 hours.
  • After incubation plates are examined for the presence of aerobic bacteria.

At Microbe Investigations, we use highest analytical performance standards to perform aerobic plate count tests. In our testing facilities, we do also offer a range of other antibacterial and antiviral test methods that validate the antimicrobial efficacy of many products. Our microbiology experts ensure all critical steps of microbiology assays are performed under optimum sterilization conditions.

We are committed to providing the highest level of quality and accuracy through our testing services. For any query regarding Aerobic Plate Count test, contact our expert to get a free consultation.


Aerobic Plate Count test is performed to detect the number of mesophilic aerobic bacteria in a given sample.

Aerobic Plate Count test is used for the microbiology analysis of read-to-use, frozen, chilled, and precooked food items. It is also applicable to indicate the bacterial populations in beverages and water samples.

Aerobic Plate Count test takes 3-4 days to complete.

At Microbe Investigations, we test for the Aerobic Plate Count using the following bacterial and fungal strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017).

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