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ASTM E2180 Test

Determines the Antimicrobial Activity In Polymeric or Hydrophobic Materials

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ASTM E2180 – Standard Test Method For Determining The Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials

ASTM E2180 is a quantitative test method for the evaluation of the antimicrobial efficacy of agents incorporated onto flat (two-dimensional) hydrophobic or polymeric surfaces. This test method is also applicable to assess the “shelf-life” or long-term durability of antimicrobial products.

Advantages of ASTM E2180 Test Method

Hydrophobic surfaces have either very low or no affinity for water. When water or aqueous solutions are spread over such surfaces, they tend to bead up into a sphere and roll away.

For antimicrobial testing of hydrophobic products, it becomes difficult to layer bacterial inoculum on treated surfaces. This subsequently affects the accuracy of results after antimicrobial evaluation. To overcome this challenge, ASTM E2180 test method is developed to check the antimicrobial activity of hydrophobic surfaces. This test standard utilizes an agar slurry inoculum vehicle which facilitates even contact between microorganisms and antimicrobial-treated hydrophobic surfaces.

ASTM E2180 Test Method

ASTM E2180 Test Procedure

  • Agar slurry inoculated by test microorganisms is spread onto the test (treated) and control (untreated) sample of hydrophobic material.
  • After a contact period of 24 hrs, microorganisms from both control and test samples are recovered separately using a neutralizing solution.
  • Serial dilutions are made and plated on agar medium. These plates are further incubated for 48 hrs under specific conditions optimal for the growth of test organisms.
  • Bacteria colonies are counted and antimicrobial activity is calculated by comparing the reduction of bacterial colonies in treated samples with bacteria colonies present on untreated samples.

Microbial Investigations (MIS) offers a wide range of antimicrobial testing services for hydrophobic materials according to international standards. ASTM E2180, ISO 22196, JIS Z 2801, and ISO 21702 are the most frequently requested test methods by our clients for their antimicrobial hydrophobic formulations.

To get a quote and rapid microbiology testing services, contact our experts now. Here we are highly focused on delivering high-quality results in a timely fashion.


ASTM E2180 test is a quantitative method for determining the antimicrobial effectiveness of the agent(s) incorporated in polymeric or hydrophobic surfaces. This test method can also be used to determine “shelf life” or long-term durability of antimicrobial-treated materials by testing both washed and non-wasted samples over a period of time.

ASTM E2180 test is applicable for polymeric materials such as plastic materials, vinyl pool liners, shower curtains, and various medical devices.

ASTM E2180 test takes 3-4 days to complete.

At Microbe Investigations, we test for the ASTM E2180 using the following bacterial and fungal strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017).

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