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EN 13727

Evaluation of Bactericidal Activity of Chemical Disinfectants used in the Medical area

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BS EN 13727:2012+A2:2015 Evaluation of Bactericidal activity of Chemical Disinfectants used in the Medical area (phase 2, step 1)

EN 13727 is a quantitative suspension test that determines the bactericidal activity of chemical disinfectants used in the medical area. The common areas where disinfection is medically indicated include patient care settings, nursing homes, dental facilities, clinics of schools, etc.

EN 13727 test ensures that the given disinfectant formulation should be effective against a range of pathogenic bacteria to meet its bactericidal efficacy claims under practical applications.

This test is applicable to a myriad of commercial products that are used in medical care facilities for disinfection –

  • Hygienic handrub, 
  • hygienic handwash, 
  • surgical handrub, 
  • surgical handwash, 
  • instrument disinfectants 
  • and surface disinfectants.

EN 13727 Test Conditions & Requirements

Test Conditions 

Hygienic handrub and handwash

Surgical handrub and handwash

Instrument disinfection

Surface disinfection

Mandatory  test organisms

Pseudomonas aeruginosa (ATCC 15442)

Staphylococcus aureus (ATCC 6538P)

Enterococcus hirae (ATCC 10541)

E. coli (K 12)

Pseudomonas aeruginosa (ATCC 15442)

Staphylococcus aureus (ATCC 6538P)

Enterococcus hirae (ATCC 10541)

E. coli (K 12)

Pseudomonas aeruginosa (ATCC 15442)

Staphylococcus aureus (ATCC 6538P)

Enterococcus hirae (ATCC 10541)

when temperature is 40 °C or higher: only E. faecium

Pseudomonas aeruginosa (ATCC 15442)

Staphylococcus aureus (ATCC 6538P)

Enterococcus hirae (ATCC 10541)

Additional 

Any relevant test organism

Test  temperature

According to the manufacturer’s recommendation, but at/between 

20 °C and 20 °C

20 °C and 20 °C

20 °C and 70 °C

4°C and 30 °C

Contact time 

According to the manufacturer’s recommendation 

but between

But no longer than

30 s and 60 s

1 min and 5 min

60 min

5 min or 60 min

Interfering substance  clean conditions 

0,3 g/l bovine albumin solution (hygienic handrub)

0,3 g/l bovine albumin solution (surgical handrub)

0,3 g/l bovine albumin solution 

3,0 g/l bovine albumin solution plus 3,0 ml/l erythrocytes

Interfering substance – dirty conditions 

3,0 g/l bovine albumin solution plus 3,0 ml/l erythrocytes (hygienic handwash) 

3,0 g/l bovine albumin solution plus 3,0 ml/l erythrocytes (surgical handwash)

3,0 g/l bovine albumin solution plus 3,0 ml/l erythrocytes

3,0 g/l bovine albumin solution plus 3,0 ml/l erythrocytes

Log reduction

≥ 5 for handrub

≥ 3 for hygienic hadnwash

≥ 5 

≥ 5 

≥ 5 

EN 13727 Test Method

  • Test sample is exposed to bacterial suspension in the presence of an interfering substance. 
  • Mixture is maintained at specific contact period time and under other mandatory conditions
  • After the end of contact period, 1 ml of mixture is neutralized.
  • Neutralizing extract is incubated for 2 days and viable colonies are enumerated. 
  • Reduction in viable colonies is compared with original culture size.

MIS has introduced a comprehensive range of microbiology efficacy testing for chemical disinfectants and antiseptics claiming bactericidal properties. In addition to EN 13727,  we are also capable of doing disinfectant efficacy testing using  EN 13697.

In antiviral efficacy testing of chemical disinfectants and antiseptics, EN 14476 is a very frequently performed test method in our laboratory.

Looking for microbiology testing services, contact us here and get an expert consultation.

FAQs

EN 13727 standard evaluates bactericidal activity of chemical disinfectant and antiseptic products used in medical area.

Products that can be tested with EN 13727 test include hygienic handrub, hygienic handwash, surgical handrub, surgical handwash, instrument, and surface disinfectant formulations.

 EN 13727 test takes 1-2 weeks to complete.

At Microbe Investigations, we test for EN 13727 using the following bacterial and fungal strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017).

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