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AOAC Use Dilution Test

(AOAC 955.14, 955.15, 955.17, 964.02): Disinfectants

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AOAC Use Dilution Test for Disinfectants

AOAC Use Dilution Test was first developed in 1955. It is a laboratory assay used to determine the efficacy of disinfectants on inanimate surfaces. This test method is specified by US EPA as the required method for validating disinfectant claims. 

AOAC Use Dilution Disinfectant test method uses “penicylinders” as carriers. These carriers are inoculated with test organisms and allowed to dry out. After this, inoculated carriers are suspended in disinfectant solution and finally transferred to broth growth medium to check the remaining test microorganisms.

Based on the type of the test organisms, AOAC Use Dilution Test methods can be selected and performed –

  • AOAC 955.14 – Salmonella enterica
  • AOAC 955.15 – Staphylococcus aureus 
  • AOAC 955.17 – Trichophyton mentagrophytes
  • AOAC 964.02 – Pseudomonas aeruginosa 

AOAC Use Dilution Test (AOAC 955.14, 955.15, 955.17, 964.02) is a very potent test for disinfectant products. In order to pass AOAC Use Dilution Test, disinfectant samples must possess good biocidal activity on a shorter contact period.

Test process of AOAC 955.14; AOAC 955.15 and AOAC 964.02
Test Process of AOAC 955.17

AOAC Use Dilution Test Method

  • 48 hours old culture of test microorganisms is inoculated and dried on stainless steel mini cylinders (penicylinder), referred to as carriers. 
  • 60 carriers are required for testing. 
  • For product samples labeled as one step cleaner/disinfectant, the organic soil is added to the culture before performing the test.
  • Three carriers are enumerated to determine pre-test carrier microbial concentrations before transferring to disinfectants.
  • Each dry and contaminated test carrier is individually transferred into 10 mL of disinfectant and exposed for specific contact period time. 
  • At the end of contact period, carriers are immediately transferred to neutralizer/growth medium. Three carriers are enumerated immediately to determine post-test carrier microbial concentrations. While other treated carriers are incubated for 48 hours.
  • After incubation, treated carriers are enumerated to estimate the number of viable cells.
  • Results are calculated and compared to following values – 
  1. For Salmonella enterica – At least 59 of the carriers must demonstrate complete disinfection
  2. For Pseudomonas aeruginosa – At least 54 of the carriers must demonstrate complete disinfection
  3. For Staphylococcus aureus – At least 57 of the carriers must demonstrate complete disinfection

Microbe Investigations (MIS) has world-class equipments and facilities based on the latest technology to perform AOAC Use-dilution test.

For more information on AOAC disinfectant testing method, reach out to our experts here. We would be happy to provide you with a quote and solve other related queries.

We are constantly betrothed in providing excellent customer service and support, ensuring that our testing analysis meets your needs and expectations.


Use Dilution test is used to measure the efficacy of liquid based disinfectants against Salmonella enterica, Staphylococcus aureus, Trichophyton mentagrophytes, and Pseudomonas aeruginosa particular.

AOAC Use Dilution test is applicable to liquid based disinfectants used on hard surfaces.

The AOAC Use Dilution test takes 4-5 days to complete.

We test for AOAC Use Dilution using the following microbial strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.

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