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AOAC 965.12

Determine Tuberculocidal Activity of Disinfectant

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AOAC 965.12 – Tuberculocidal Activity of Disinfectants

AOAC 965.12 test is used for validation of tuberculocidal activity of disinfectants intended for application on non – porous inanimate surfaces.

AOAC 965.12 specifies a carrier based test method, which involves the carrier surface being contaminated with challenging microorganisms and then challenged with the test product. Contaminated test surface represents the actual conditions of product usage, hence helping to better understand product performance. 

Test process of AOAC 965.12

AOAC 965.12 Test Method

  • A culture of Mycobacterium bovis BCG, which is cultivated for 21 days is taken for the experiment.
  • 10 carrier surfaces are required for this experiment.
  • A test suspension of microorganisms with interfering substance is inoculated onto porcelain test surfaces (carriers) and allowed to dry.
  • Dried contaminated surfaces are transferred to a container containing disinfectant solution. This is kept to interact for specific times.
  • At the end of contact times, the treated test surfaces are taken out and transferred to a neutralizing solution to halt further reaction.
  • In the next step, treated test surfaces are placed into the bacterial growth medium for an incubation period of 60 days.
  • After incubation period, test surfaces are observed for bacterial growth. If there is no detectable growth on all 10 test surfaces, they are incubated for an additional 30 days.

Importance of AOAC 965.12 Test

Mycobacteria are microorganisms that can survive on inanimate surfaces for extended periods and remain infectious. This poses a significant risk of acquiring nosocomial infections in places like hospitals, laboratories, and other environments.

Due to their complex cell wall structure, mycobacteria exhibit resistance to regular disinfectants. Therefore, specialized disinfectants containing tuberculocidal agents are necessary to effectively eliminate them.

In the US, Environmental Protection Agency (EPA) regulates disinfectant products labeled
as tuberculocidal and recommends employing AOAC 965.12 test method to validate product efficacy claims.

Looking for AOAC 965.12 testing services, a team of MIS experts is here to help you! At MIS, we offer a comprehensive range of AOAC testing solutions that are tailored as per clients requirements.

We completely understand the significance of microbiology analysis in the process of development of high quality products. Therefore, our experts follow standard guidelines to ensure quality and accuracy in their testing procedures.

In addition to evaluating tuberculocidal activity of disinfectants, we perform AOAC 965.13 test for pool and spa disinfectants and field testing.

To get a quote on AOAC 965.12 test or want to know more about our testing services, contact our experts here.


AOAC 965.12 test is used to validate tuberculocidal activity of disinfectants sold in US markets. Mycobactericidal efficacy of product samples is tested on porcelain test surfaces (referred as carriers) against Mycobacterium bovis.

AOAC 965.12 testing is applicable to non – porous surface disinfectants that claim to possess tuberculocidal activity.

AOAC 965.12 test takes 4-5 months to complete.

At Microbe Investigations, we test for AOAC 965.12 using the following microbial strains – Mycobacterium bovis.

Contact us for more information

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