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AOAC 960.09

Germicidal and Detergent Sanitizing Action of Disinfectants

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AOAC 960.09 – Germicidal and Detergent Sanitizing Action of Disinfectants

AOAC 960.09 method is employed to determine the minimum concentration of chemicals that can be used for sanitizing precleaned, nonporous food contact surfaces.

AOAC 960.09 is an EPA-specified method that is particularly applicable for dilutable sanitizers that do not utilize halogens like chlorine and bromine.

Test Process of AOAC 960.09

AOAC 960.09 Test Method

  • Culture of test micrograms (gram + ve / gram -ve) are cultivated in appropriate broth and then standardized to 1 x 10^10 cfu/ml.

  • For each microorganism, 1 ml of test suspension is inoculated into an Erlenmeyer flask containing 99 ml of test product. This is allowed to interact for the specified contact time.

  • For control test, 1 ml of test suspension is inoculated into an Erlenmeyer flask containing 99 ml of a non-antiseptic buffer to determine the initial concentration of microorganisms.

  • At the end of contact time, a small portion of (product + test suspension) is added to neutralizing solution and followed by dilution and plating to determine surviving microorganisms.

  • After the incubation period, reduction in microbes found in the test sample is compared with microbes obtained from the control sample.

  • In order to pass AOAC 960.09, the test product must be able to kill more than 99.999% of both Gram-positive and Gram-negative microorganisms.

Importance of AOAC 960.09 Test

Maintaining proper hygiene in food preparation areas is of paramount importance to ensure the quality of food products and public health safety. Food contact surfaces serve as potential reservoirs of pathogenic microorganisms due to their frequent contact with raw ingredients, utensils, and workers.

Proper sanitization of surface areas helps in reducing the number of microorganisms, subsequently minimizing the risk of foodborne illnesses. AOAC 960.09 lays down efficacy criteria and test requirements to check the efficacy of the chemical used in the food industry for sanitizing precleaned food contact surfaces.

At MIS, we offer AOAC 960.09 testing services to support our clients in validating the efficacy claims of their products. Our testing facilities are equipped with cutting-edge technology which enable us to guarantee the utmost level of accuracy and precision in our test reports.

Furthermore, we also perform AOAC 961.02 efficacy test for germicidal spray products as disinfectants.

To get a quote on AOAC 960.09 test, contact our experts now.


AOAC 960.09 is a standard method used to evaluate the efficacy of (germicidal and/or bactericidal products) intended for sanitizing pre cleaned, non- porous food contact surfaces. To meet AOAC 960.09 efficacy criteria, the test product must kill >99.999% of both the gram-positive and gram-negative test microorganisms.

AOAC 960.09 test is applicable for germicidal and disinfectant products designed for the sanitization of precleaned food contact surfaces.

AOAC 960.09 test takes 3-4 weeks to complete.

For AOAC 960.09 testing, we customize our clients testing needs by accommodating specific stain requests. To know, check out our microbial library.

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