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AOAC 991.47

Testing disinfectants against salmonella choleraesuis

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AOAC 991.47 – Evaluation of disinfectant sample for its bactericidal activity against Salmonella choleraesuis

AOAC 991.47 test lays down a standard guideline to test the efficacy of a given disinfectant sample against Salmonella choleraesuis on a hard surface carrier.

AOAC 991.47 is an important guideline that helps to verify the performance of the disinfectant sample against Salmonella choleraesuis, a highly pathogenic species of Salmonella that causes septicemic diseases. AOAC 991.47 thus serves as the standard testing guideline for registration of the disinfectants that claim to have bactericidal efficacy against Salmonella choleraesuis as per regulatory requirements set by the US Environmental Protection Agency (EPA) and US Food and Drug Administration (FDA).

AOAC 991.47 Test Method

  • 70 carriers are used for a single test sample. Each carrier is inoculated with the test organism.
  • In the next step, 60 carriers contaminated with test microorganisms are exposed to the test product for specific contact times and the remaining 10 are kept as untreated controls.
  • At the end of contact time, treated carriers are immediately transferred to a recovery medium enriched with neutralizing compounds.
  • Recovery medium is then incubated for a comprehensive qualitative assessment of any potential growth that may still be present after disinfectant treatment.

At MIS, we offer an extensive portfolio to test the bactericidal efficacy of a diverse range of disinfectant products.

Our team comprises highly skilled microbiologists who possess in-depth knowledge and experience in AOAC testing analysis.
Under our AOAC testing portfolio, we also perform an AOAC 965.12 test for the tuberculocidal activity of disinfectants.

To get a quote on AOAC 991.47 testing, talk to our experts today.


AOAC 991.47 is a carrier-based test that helps to validate the bactericidal performance of a disinfectant sample against Salmonella choleraesuis.

AOAC 991.47 test is applicable to water-soluble powders and non-volatile liquid products intended for the disinfection of hard surfaces against Salmonella choleraesuis.

AOAC 991.47 test takes 4-5 weeks to complete.

We test for AOAC 991.47 using Salmonella choleraesuis.

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