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EN 16437

Determine Bactericidal activity of Chemical Disinfectant & Antiseptics

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BS EN 16437 : 2014 + A1 2020 – Evaluation of Bactericidal activity of Chemical Disinfectants

EN 16437 specifies (phase 2 step 2 carrier test) a Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in veterinary area on porous surfaces without mechanical action.

Regular cleaning of critical surfaces in veterinary settings is a must to avoid outbreak of pathogens. Thereby, efficacy of the products used as veterinary disinfectants/antiseptics must be checked against the representative microorganisms they are intended for use against. EN 16437 standard provides regulatory procedures for registration of veterinary disinfectants in Europe.

EN 16437 Test Conditions & Requirement

  • Mandatory test microorganisms  – Enterococcus hirae (ATCC 10541), Proteus vulgaris, Pseudomonas aeruginosa (ATCC 15442), Staphylococcus aureus (ATCC 6538P)
  • Test temperature  – 10°C 
  • Contact time  – Between 1 minute and 60 minutes (in this range, contact times of  5,  15,  30, 60 and 360 min can be selected)
  • Interfering substance  – 3g/l bovine albumin solution 
  • Passing criteria  – Product must demonstrate >4 log reduction value
Test method of EN 16437

EN 16437 Test Method

  • 10ul of bacterial suspension with interfering substance is inoculated on the surface of a poplar wood placed in a sterile petri dish and allowed to dry at  37°C.
  • Dried test surface is then exposed to product sample for a contact time of 1 minute.
  • After the contact time, the test surface is transferred to a new sterile petri dish and incubated for a specific time period. 
  • In the next step, test surface is further transferred to a vessel containing a neutralizing solution. After vortexing for 15 seconds, the mixture is placed in a water bath at 20°C for 25 minutes. Vessel is placed in an ultrasonic bath to remove bacteria from the test surfaces.
  • Neutralizing solution is acquired, plated, and incubated to enumerate remaining bacteria.

At MIS, we provide antimicrobial testing solutions to industries and clients around the world. We design protocols and execute antimicrobial efficacy tests for a wide range of disinfectant and antiseptic formulations. 

In our lab, EN 16437 and EN 1656 are frequently requested and performed tests under veterinary disinfectant testing,

With deep industry expertise and cutting-edge competencies, we provide clients with reliable and responsive services. For more detailed information on our EN 16437 testing services, contact our experts here.


EN 16437 specifies quantitative surface test to determine whether a given disinfectant and antiseptic product has bactericidal activity on a porous surface without mechanical action in the veterinary area. For a product to pass EN 16437 test, it  must demonstrate a minimum of 4 log reduction value.

EN 16437 test is applicable for chemical disinfectants and antiseptics designed for disinfection of porous surfaces in veterinary areas.

EN 16437 test takes 2-3 weeks to complete.

At Microbe Investigations, we test for EN 16437 using the following microbial strains: Enterococcus hirae (ATCC 10541), Proteus vulgaris, Pseudomonas aeruginosa (ATCC 15442), Staphylococcus aureus (ATCC 6538P).

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