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ASTM e2197

Quantitative Disk Carrier Test

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ASTM E2197 : 2017 – Quantitative Disk Carrier Test for Determination of Bactericidal, Virucidal, Fungicidal, Mycobactericidal, and Sporicidal Activities of Chemical Germicides

ASTM E2197 is designed to evaluate the performance of germicidal formulations in killing or inactivating bacteria, viruses, fungic, bacterial spores, and mycobacteria.

ASTM E2197 standard uses stainless steel disks as carriers, mimicking real conditions for non – porous environmental surfaces and medical devices. The performance of the test product is interpreted by comparing the number of viable test microorganisms with respect to control carriers.

The test protocol does not incorporate any wiping or rubbing actions, hence, it is not utilized for assessing the effectiveness of germicide-soaked wipes.

ASTM E2197 Test Conditions & Requirements

  • Mandatory test microorganisms Staphylococcus aureus (ATCC 6538P), Pseudomonas aeruginosa (ATCC 15442), Aspergillus niger (ATCC-16888), Candida Albicans (MTCC 3017), Mycobacterium terrae, Bacillus subtilis, Clostridium sporogenes and Clostridium officinale.

  • Contact time and temperature As per manufacturer’s recommendations

  • Interfering substance Tryptone + Bovine Serum Albumin + Mucine

ASTM E2197 Test Method

  • Test microorganisms are inoculated to the center of stainless steel disks (1 cm in diameter).
  • Inoculated carriers are allowed to dry under vacuum at room temperature.
  • In the next step, dried inoculated carriers are individually placed into the bottom of sterile vessels.
  • Test substance is added directly to the center of the dried inoculated carrier and allowed to interact for specific contact times.
  • After contact time, neutralizer/elution media is added to each vessel and vortexed to separate any viable test microorganisms from carriers.
  • The resulting eluent is passed through a membrane filter to ensure the screening of even minimal numbers of viable microbes.
  • Membrane filter is further rinsed with saline to reduce the chances of carrying any inhibitory residues to the recovery medium that can interfere with the test.
  • The filtrate is plated and incubated to determine the number of viable microorganisms.
  • After incubation, the number of colony forming units (CFU) is averaged and converted to log 10 and microbicidal activity of the test sample is compared to the control sample.

Importance of ASTM E2197 Test

Germicidal products are commonly used for surface disinfection in hospital settings, households, food preparation areas and other similar environments. Such products effectively destroy or eliminate bacteria, viruses, fungi, and bacterial spores that can lead to infections. Therefore, in order to ensure a high level of cleanliness and hygiene in infection prone areas, it is important to use germicidal products which have passed the efficacy test for their intended use.

ASTM E2197 test method provides, efficacy criteria, and result data to understand product efficacy and facilitates enhancement in product performance prior to its market launch.

MIS offers a comprehensive range of germicidal efficacy tests for a variety of liquid formulations. We also provide tailored/ customized services to our clients. Our team of experts is highly proficient in creating protocols that adhere to the international standards like ASTM, AOAC, EN, and ISO.

We ensure microbiological interpretation of our test results are accurate, reliable, and delivered within a defined time frame to our clients. Also check, ASTM E1053 and ASTM E2362 are other frequently performed ASTM standards in our labs.

Looking for ASTM E2197 testing services, contact our microbiology team experts today.


ASTM E2197 is a germicidal efficacy test method. This method is used (referred as carriers) to evaluate the effectiveness of tested products against bacteria, viruses, fungi, and mycobacteria.

ASTM E2197 test method is applicable for germicidal products like disinfectant and antiseptics utilized for surface disinfection in critical areas.

ASTM E2197 test takes 3 – 4 weeks to complete.

At Microbe Investigations, we test for ASTM E2197 using the following microbial strains: Staphylococcus aureus (ATCC 6538P), Pseudomonas aeruginosa (ATCC 15442), Aspergillus niger (ATCC-16888), Candida Albicans (MTCC 3017), Mycobacterium terrae, Bacillus subtilis, Clostridium sporogenes, and Clostridium officinale

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