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AATCC 174 : 2022 Test Method for Antimicrobial Activity of New Carpets

AATCC 174 has been developed by American Association of Textile Chemists and Colorists (AATCC) to determine the antimicrobial activity of new carpet materials. This standard is also applicable to analyze the effect of a cleaning process on the antimicrobial resistance of carpets.

Organisms typically used in this method: Staphylococcus aureus (ATCC 6538P)Klebsiella pneumoniae (ATCC 4352), Apergillus niger(ATCC 16888)

AATCC 174 covers the following three antimicrobial test procedures –

1) AATCC 174 Test I  – Qualitative Antibacterial Assessment, Single Streak Method

  • Molten agar medium is poured onto sterile petri plates and allowed to solidify. Staphylococcus aureus (ATCC 6538P) and Klebsiella pneumoniae (ATCC 4352) are mandatory test microorganisms in AATCC 174 Test I and AATCC 174 Test II.
  • Using a sterile inoculating loop, test microorganisms are streaked over the surface of agar medium.
  • Both treated (test ) and untreated (control) carpet samples are placed transversely across inoculated streaks.
  • After 24 hours of incubation, plates are examined for zones of inhibition.

2)  AATCC 174 Test II – Quantitative Assessment of Antibacterial Activity on Carpets

  • Bacterial inoculum is spread onto pre-wetted treated (test) and untreated (control) carpet samples. 
  • Immediately after inoculation (0 contact times), bacterial enumeration is done for control samples followed by neutralization, plating, and incubation. 
  • Other test samples are incubated for 24 hours. 
  • After incubation, neutralizing solution is added to test samples to extract remaining bacteria. And plate count method is used to enumerate the number of viable bacteria present in sample.
  • Reduction in the number of viable bacteria in test sample is compared with bacterial count recovered at 0 contact times.

3) AATCC 174 Test  III – Qualitative  Antifungal Assessment (Mildew and Rot Resistance of Carpet Materials)

  • Aspergillus niger (ATCC-16888) is test microorganism in AATCC 174 Test  III.
  • Both treated and untreated carpet samples are placed on agar surface which is inoculated with test microorganisms.
  • Plates are kept for 7 days incubation period and examined for fungal growth.

At MIS, we offer a wide array of testing analysis services, primarily to industries that are building antimicrobial textiles, disinfectants, paints and coatings.

AATCC 174 testing protocols are strategized and implemented by our highly experienced microbiology experts. We utilize cutting-edge technologies and methodologies to achieve greater accuracy in results of our analytical processes.

To get a quote on AATCC 174 test method, please contact our experts now.


AATCC 174 specifies antibacterial and antifungal test methods to assess the efficacy of carpet materials.

AATCC 174 test is applicable for carpet materials.

AATCC 174 test takes 3 – 4 weeks to complete.

At Microbe Investigations, we test for AATCC 174 using the following bacterial and fungal strains: Staphylococcus aureus (ATCC 6538P), Klebsiella pneumoniae (ATCC 4352) and Aspergillus niger (ATCC-16888). Additional strains tested at MIS inlcude Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Enterococcus hirae (ATCC 10541), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Aspergillus niger (ATCC-16888), Candida Albicans (MTCC 3017).

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