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EN 17915

Evaluation of Virucidal Activity of Chemical Disinfectants on Hard Non-Porous Surfaces

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BS EN 17915 : 2022 – Test method for the evaluation of virucidal activity of chemical disinfectants on hard non-porous surfaces in food, industrial, domestic, and institutional areas (phase 2 step 2)

EN 17915 standard specifies the test method to determine virucidal activity of disinfectants on non – porous surfaces without mechanical action. EN 17915 test is applicable to disinfectant products used in food, industrial, domestic, and institutional areas, except for the situations where disinfection is medically indicated and excluding products used on living tissues.

EN 17915 is a carrier test method (phase 2, step 2) that aims at determining the efficacy of disinfectants under more realistic conditions.

EN 17915 Test Conditions & Requirement

Minimum test conditions and parameters specified by EN 17915 standard are as follows –

Minimum conditions

Surfaces disinfection in industries, food processing, distribution and retailing


Surfaces disinfection in institutional

and domestic areas

Mandatory test organisms

Virucidal activity for biopharmaceutical industry 


Murine Parvovirus


Virucidal activity for industries other than biopharmaceutical and food industry


 Adenovirus (ATCC VR-5)


 Murine Norovirus (FLI S99)


Virucidal activity for food industry


    Murine Norovirus (ATCC VR-5)


 Virucidal activity against enveloped viruses 


 Vaccinia virus (ATCC       VR-1549)


Virucidal activity (chemo-thermal)


Murine Parvovirus for temperatures >40°C

Virucidal activity for institutional

and domestic use


Adenovirus (ATCC VR-5)


Murine Norovirus (FLI S99)


Virucidal activity against enveloped viruses


Vaccinia virus (ATCC VR-1549)

Test temperature

between 18 °C and 25 °C but for chemothermal activity 

between 18°C and 25 °C

Additional temperature 

between 4 °C and ≤ 40 °C

between 4 °C and and ≤ 40 °C

Contact time

As per manufacturer’s recommendations, but not longer than 60 min

As per manufacturer’s recommendations, but not longer than 60 min

Interfering substance


Clean conditions


0,3 g/l bovine serum albumin


0,3 g/l bovine serum albumin

Dirty conditions

3,0 g/l bovine serum albumin

3,0 g/l bovine serum albumin

Test Process of EN 17915

EN 17915 Test Method

  • Test suspension of virus with interfering substance is inoculated onto a test surface and dried out.
  • Disinfectant sample is evenly spread on dried microbial film and allowed to interact for specific contact times. In parallel, a control test is run wherein the virus infected test surface is treated with hard water instead of disinfectant.
  • At the end of contact time, the test surface is immediately transferred to the cell maintenance medium to neutralize further activity of the disinfectant sample.
  • Infectious titre of virus recovered in cell maintenance medium is determined and compared with infectious titre from control test.

Importance of EN 17915 Test

Many viruses exhibit the ability to survive on hard surfaces for an extended period and remain infectious. The process of disinfection plays a crucial role in elimination of harmful viruses and ensures a safe and hygienic environment. Not all available disinfectants are capable of effectively killing viruses. It is essential to use disinfectants formulated with virucidal agents for effective virus disinfection.

As per European norms, disinfectants claiming virucidal activity must be tested for their efficacy under standardized methods. The European Standard EN 17915 has been devised to check the virucidal efficacy of non-porous surface disinfectants before their intended applications.

MIS performs routine virucidal testing on disinfectants and antiseptics utilized for diverse application areas including food, industrial, domestic, institutional, medical, and veterinary settings. 

Our cutting-edge facility offers antiviral efficacy research globally, catering to products with antiviral potential. 

Explore,  EN 16777 test for virucidal activity of chemical disinfectants at MIS lab.

To get a quote on  EN 17915 test or to learn more about our testing capabilities, please contact our experts today.


EN 17915 describes phase 2, step 2 test method to assess virucidal efficacy of non-porous surface disinfectants utilized in food, industrial, domestic, and institutional areas. The EN 17915 test method stimulates more real conditions by using a test surface (career) to determine the virucidal potential of disinfectant samples.

EN 17915 test is applicable to products used for disinfection of non-porous surfaces. These products are commonly used across various sectors such as food, industry, households, and institutions.

EN 17915 test takes 4-5 weeks to complete.

At Microbe Investigations, we test for EN 17915 using the following microbial strains: Adenovirus (ATCC VR-5), Murine Norovirus (FLI S99), Vaccinia virus (ATCC VR-1549), and Murine Parvovirus. Additional virus strains can be tested on customer’s request.

Contact us for more information

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