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EPA #01-01A

Protocol for Residual Self-Sanitizing Activity of Dried Chemical Residues on Hard, Non-Porous Surfaces

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EPA #01-01A: Standard Method for the Evaluation of Residual Self-Sanitising Activity of Dried Chemical Residues on Hard, Non-Porous Surfaces

EPA #01-01A Test determines the residual sanitizing efficacy of antimicrobial products applied onto hard, non-porous contact surfaces against test bacteria. The method is used to assess the residual sanitizing efficacy upon mechanical abrasion of the coatings on the hard, non-porous surfaces against microbes such as Staphylococcus aureus (ATCC 6538), Klebsiella pneumoniae (ATCC 4352), Enterobacter aerogenes (ATCC 13048), Escherichia coli 0157:H7 and Salmonella cholerasuis. However, the method can also be adapted to test against other bacterial strains as well.

EPA #01-01A Test Method is divided into two parts:

Part 1: Preparation and Abrasion Treatment

  1. Glass, metal, and plastic carriers are coated with the test product for a minimum of 3 hrs and act as the test specimens. Uncoated glass, metal, and plastic carriers act as controls for each material respectively.
  2. The carriers are inoculated with the test microorganism culture that is 48-56 hr old.
  3. All carriers then undergo an alternating wet and dry abrasion treatment over a 24-hour duration.
  4. Each treatment lasts for 15 minutes and is followed by a 15-minute rest period, after which the carriers are re-inoculated with the test microorganism, followed by a 30-minute drying period.
  5. A total of 12 wear cycles and 5 re-inoculations are performed for each test microorganism.

Part 2: Product efficacy

  1. Following the completion of the abrasion and reinoculation treatment, the carriers are challenged with a 10μL inoculum of the test microorganism and soil load for a contact time of 5 minutes.
  2. After the contact time, the antimicrobial action of the test specimen is neutralized using a neutralizing media, and the surviving test organism is recovered.
  3. The number of viable microorganisms recovered from the controls (uncoated and unabraded) and test specimens (coated and aberrated) is determined quantitatively.
  4. The number of viable microorganisms on treated and abraded carriers is compared to the untreated control carriers and the residual sanitising efficacy is determined in the form of log reduction.

A test product on the carriers must reduce the total number of microorganisms by at least 99% on the carrier surface

Test Process of EPA - 01 - 1A

Importance of the EPA #01-01A Test Method:

EPA #01-01A testing provides supporting efficacy data for long-term residual claims for the sanitizing activity of the antimicrobial surface coating of hard, non-porous surface products. This method applies to products used on inanimate, non-food contact hard surfaces. These products may be sprayed or applied otherwise. The abrasion treatment represents normal wear and tear when exposed to biocidal materials.

Here at MIS, we develop and optimize test protocols for EPA #01-01A which comply with international standards and appropriate safety protocols are followed while testing surface coatings against human pathogenic microorganisms.

MIS also offers a test for evaluating the efficacy of antimicrobial surface coatings (EPA MLB SOP MB-40) and for hard non-porous Copper-containing surface products (EPA MLB SOP MB-41).

To get a quote on the EPA #01-01A Test Method or to know more about the test in detail with regards to your product please reach out to our experts here.


It is a test that evaluates the residual sanitizing efficacy of antimicrobial products applied to non-porous, contact hard surfaces.

Products used on inanimate, non-food contact hard surfaces can be tested for residual sanitising activity using this method.

At present, Staphylococcus aureus, Klebsiella pneumoniae, Enterobacter aerogenes, Escherichia coli, and Salmonella cholerasuis can be tested. The test can be adapted for other microorganisms.

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