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Evaluate the Efficacy of Antimicrobial Surface Coatings

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EPA MLB SOP MB-40: Standard Method to evaluate the efficacy of antimicrobial surface coatings

EPA MLB SOP MB-40 Test determines the efficacy of antimicrobial surfaces coated against test bacteria and viruses. The method is used to assess the residual antimicrobial efficacy upon mechanical abrasion of coatings on hard, non-porous surfaces against prescribed microbes such as Staphylococcus aureus, Pseudomonas aeruginosa, and Viruses. However, the method can also be adapted to test against other bacterial strains

EPA MLB SOP MB-40 Test method is divided into two parts:

Part 1: Chemical treatment and physical abrasion

  • Stainless steel carriers of 1” × 1” brush-coated with antimicrobial chemicals act as test specimens. While uncoated carriers act as controls.
  • A total of 4 types of treatment are performed for any given antimicrobial sample.
  • 3 types of treatment involves the carrier to be abraded using 5 cycles of alternating wet and dry abrasions.
  • The fourth treatment involves 10 cycles of dry abrasions.

Part 2: Product efficacy

  • After the completion of the treatment and abrasion, the carriers are challenged with a 20ul mixture of test organism and soil load for a contact time of 1-2 hrs.
  • After the contact time, the antimicrobial action of the test specimen is neutralized using a neutralizing media, and the surviving test organism is recovered.
  • The number of viable microorganisms recovered from the controls (uncoated and unabraded) and test specimens (coated and aberrated) is determined quantitatively.
  • The number of viable microorganisms on treated and/or abraded carriers is compared to the untreated control carriers and the antimicrobial efficacy is determined in the form of log reduction.

A minimum of 3 log reduction in microorganisms within 1-2 hrs indicates a 1-week residual claim for the antimicrobial surface coating.

Test Process of EPA MLB SOP MB - 40

Importance of the EPA MLB SOP MB-40 test Method:

EPA MLB SOP MB-40 testing can provide supporting efficacy data for the registration of surface coatings used to supplement standard disinfectants to augment their antimicrobial activity. The chemical treatments and abrasions performed in the test are intended to represent normal wear & tear and repeated exposure to chemical biocides.

Here at MIS, we develop and optimize test protocols for EPA MLB SOP MB-40, which comply with international standards and follow appropriate safety protocols while testing surface coatings against human pathogenic microorganisms.

MIS also offers a test for evaluating the antimicrobial activity of hard, non-porous Copper-containing surface products (EPA MLB SOP MB-41)

To get a quote on the EPA MLB SOP MB-40 Test Method or to know more about the test in detail with regards to your product please reach out to our experts here.


It is a test that evaluates the efficacy of antimicrobial-coated surfaces, that are used to supplement standard disinfectants, against test bacteria and viruses.

All antimicrobial surface coatings used on hard, non-porous surfaces can be tested.

At present, Staphylococcus aureus, Pseudomonas aeruginosa, and viruses can be tested. The test can be adapted for other microorganisms.

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