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EN 17914

Evaluation of Virucidal Activity of Chemical Disinfectants and Antiseptics

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BS EN 17914: 2022 Quantitative suspension test method for evaluating the virucidal activity of chemical disinfectants and antiseptics in food, industrial, domestic, and institutional areas

BS EN 17914: 2022 is a phase-2 step-1 quantitative suspension test method that lays out a guideline for assessing the virucidal activity of a given chemical disinfectant or antiseptic intended to be used in the –

  • Food
  • Industrial
  • Domestic
  • Institutional areas.

The test parameters have been formulated to closely mimic the practical application condition for the disinfectant. The standard is applicable to a test product that forms a homogeneous, physically stable preparation when diluted with hard water – or in the case of ready-to-use products, i.e., products that are not diluted when applied – with water.

EN 17914 Test Conditions & Requirements:

Test Conditions 

Hygienic handrub  

and handwash

Instrument  

disinfection

Surface disinfection 

Textile 

disinfection

Mandatory  

test organisms

Non – enveloped viruses

Poliovirus 

Adenovirus 

Murine Norovirus 

Poliovirus 

Adenovirus 

Murine Norovirus 

 

when temperature is  40 °C or higher, only  Parvovirus

Poliovirus 

Adenovirus 

Murine Norovirus 

Parvovirus

 

Limited spectrum  

virucidal activity 

 

Adenovirus 

Murine Norovirus 

 

-NA –

 

Limited spectrum  

virucidal activity 

 

 Adenovirus 

 Murine norovirus

 

-NA –

Enveloped viruses

 

Vaccinia virus

 

-NA –

 

 

 

vaccinia virus

-NA –

 

Additional 

Any relevant test organism

 

Test  temperature

According to the manufacturer’s recommendation, but at/between 

20 °C

20 °C and 70 °C 

4 °C and 30 °C

30°C and 70 °C

Contact time 

According to the manufacturer’s recommendation 

 

between 

30 s and 120 s

But no longer than

    60 min

  5 min or 60 min

    20 min

Interfering substance – clean conditions 

0,3 g/l bovine albumin

solution (hygienic

handrub)

0,3 g/l bovine albumin  solution 

0,3 g/l bovine albumin  solution 

3,0 g/l bovine  

albumin solution plus  3,0 ml/l erythrocytes

     

Interfering substance – dirty conditions 

3,0 g/l bovine albumin

solution plus 3,0 ml/l

erythrocytes (hygienic

handwash)

 

3,0 g/l bovine albumin

solution plus 3,0 ml/l

erythrocytes

3,0 g/l bovine albumin

solution plus 3,0 ml/l

erythrocytes

3,0 g/l bovine

albumin solution plus

3,0 ml/l erythrocytes

 

Test Process of EN 17914 Method

EN 17914 Test Method

  • The given test sample (disinfectant) or hard water is first mixed with an interfering substance, followed by challenging the mixture with the test virus.

  • The contact time for which the disinfectant sample is challenged and the temperature at which the mixture is incubated is as per the manufacturer’s recommendations or the standard guidelines.

  • At the end of the contact time, an aliquot (small portion) of the test mixture is taken, and the antiviral action of the disinfectant sample is terminated using a neutralizing medium.

  • The neutralizing medium with the virus is serially diluted and transferred onto a permissive monolayer of cells in a 96-well plate.

  • The 96 well plates are incubated in a CO2 incubator for a specific period of time depending on the test virus.

  • After the incubation period, 96 well plates are examined for CPE (cytopathic effect), and the number of the infective virus is calculated using the TCID50 method.

  • Antiviral efficacy of the disinfectant sample is measured by comparing the virus titer with the control (without test product) sample.

Importance of EN 17914 Test

EN 17914 guideline helps in evaluating the efficacy of disinfectant/antiseptic products in preventing the transmission and spread of any viral disease in the –

  • Food
  • Industrial
  • Domestic
  • Institutional areas.

The disinfectant sample passing the EN 17914 test will help to maintain a safe and healthy environment. EN 17914 also serves as the standard testing guideline for registering and selling the disinfectant sample in the European zone and some other countries.

Why choose MIS Lab to conduct EN 17914 testing?

MIS performs routine virucidal tests for disinfectants implied to be used in different areas of applications.

Our state-of-the-art testing facility is one of only a few facilities worldwide that provides efficacy research and data for products with antiviral/ virucidal potential.

Additionally, EN 14476, EN 17111, ASTM E 1053, and EN 16777 tests are also available for disinfectant virucidal claims in the MIS lab.

To get a quote on the EN 17914 test or if you need any additional information regarding our testing services, please contact our experts today.

FAQs

EN 17914 is a phase-2 step-1 test that specifies a suspension test method for evaluating the virucidal activity of chemical disinfectants and antiseptics used in the Food, Industrial, Domestic, and Institutional areas.

EN 17914 test applies to disinfectant/antiseptic products intended for disinfecting without mechanical action on non-porous surfaces in the Food, Industrial, Domestic, and institutional areas.

EN 17914 test takes 5-6 weeks to complete.

At Microbe Investigations, we test for EN 17914 using the following microbial strains: Adenovirus, Poliovirus, and Murine norovirus for non-enveloped viruses and Vaccinia Virus for enveloped viruses.

Contact us for more information

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