ASTM E1054 Test
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ASTM E1054 : 2022 – Evaluation of Inactivators of Antimicrobial Agents
ASTM E1054 test is designed to ensure that neutralizing agents (inactivators) used or the process followed in the antimicrobial testing methods do not exert any inhibitory effects on test microorganisms during the recovery process.
The neutralization assay selected for antimicrobial effectiveness evaluations are based on their recoveries in semi-solid media, liquid media, and by membrane filtration.
ASTM E1054 Test Method
Test A – Neutralizer Effectiveness
- This test is performed in 3 replicates.
- A test product/solution containing product is added to the neutralizer at the same dilution ratio as used in antimicrobial effectiveness evaluation.
- Immediately, the test product/ neutralizer mixture is inoculated with a measured volume of the challenge microorganism suspension s resulting suspension contains 30 to 100 CFU/mL of the microorganism.
- Above mixture is allowed to interact for specific durations as used in antimicrobial efficacy tests.
- After contact time, mixture is enumerated using quantitative pour or spread plates. Plates are incubated under the same conditions as employed in the antimicrobial effectiveness evaluation.
Test B – Neutralizer Toxicity
- This test is performed in 3 replicates.
- PBS or other appropriate buffering agent is added to the neutralizer that will result in the same dilution ratio as that used in Test A.
- Immediately, PBS/ neutralizer mixture is inoculated with a measured volume of the challenge microorganism suspension, ensuring that resulting suspension contains 30 to 100 CFU/mL of the microorganism.
- Above mixture is allowed to interact for specific durations as used in antimicrobial efficacy tests.
- After contact time, mixture is enumerated using quantitative pour or spread plates. Plates are incubated under the same conditions as employed in the antimicrobial effectiveness evaluation.
Test C – Test Organism Viability
- This test is performed in 5 replicates.
- PBS or other appropriate buffering agent is inoculated with a measured volume of the challenge microorganism suspension, ensuring that resulting suspension contains 30 to 100 CFU/mL of the microorganism.
- Above mixture is allowed to interact for specific durations as used in antimicrobial efficacy tests.
- After contact time, mixture is enumerated using quantitative pour or spread plates. Plates are incubated under the same conditions as employed in the antimicrobial effectiveness evaluation.
Importance of ASTM E1054 Test
Antimicrobial agents are added in the formulations of disinfectants, sanitizers, and antiseptics products to effectively kill or inhibit the growth of harmful pathogens. Thereby, evaluation of antimicrobial performance is necessary to demonstrate their safety and effectiveness before being marketed and sold.
Neutralization of the antimicrobial agents is a critical step for accurate evaluation of antimicrobial activity. Inefficient or incomplete neutralization leads to killing or inactivation of microorganisms beyond the intended experimental exposure time. This outcome can result in an overestimation or wrong interpretation of antimicrobial activity.
ASTM E1054 provides neutralization evaluation assays to determine whether components and methods used for neutralization interfere with activity of antimicrobial agents.
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FAQs
ASTM E1054 is designed to assess that neutralizing agents/inactivators utilized in antimicrobial testing methods do not exhibit any inhibitory effects on the test microorganisms targeted for recovery.
ASTM E1054 testing is applicable for neutralizing agents used for inactivation of antimicrobial agents.
ASTM E1054 test takes 3 – 4 weeks to complete.
The microbial strains utilized in the ASTM E1054 – neutralization assay will be the same as those employed in antimicrobial efficacy tests. The selection of these strains will be based upon the antimicrobial characteristics of the test product.
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