Disinfectant Efficacy Testing
What is disinfectant efficacy testing (DET)?
Disinfectant Efficacy Testing (DET) is the process of testing the effectiveness of disinfectants in efficiently killing or reducing microorganisms present on any surface. Disinfectant testing ensures that these products meet the specified regulatory requirements and perform as expected in real-life usage scenarios. By determining the effectiveness of the product against a broad spectrum of microorganisms, efficacy testing helps verify the effectiveness of these products in preventing infections in varied environments, especially in high-risk areas like healthcare and public settings.
Disinfectant efficacy testing ensures that the disinfectant products used are effective in eliminating harmful microorganisms from contaminated surfaces. Effective surface disinfection must be carried out in high-risk areas, such as hospitals and public places, to avoid any potential spread of pathogens. Furthermore, testing assures health facilities and public health administrators that the products they trust for disinfection work as intended.
Evaluation of product efficacy
Several international standards provide strict guidelines for assessing the bactericidal, fungicidal/yeasticidal and virucidal efficacy of chemical disinfectants. These regulatory standards include the European Standards (EN ), the International Organization for Standardization (ISO), the American Society for Testing and Materials (ASTM) and the Association of Official Agricultural Chemists (AOAC ) standards. Disinfectant manufacturers are recommended to consider the requirements of each organization and their geographical locations before choosing the test standard, as these requirements may vary by region. The evaluation criteria depend on the intended use of the product and the test conditions specified in standard guidelines.
Disinfectant Products Tested
Disinfectant and antiseptic formulations used across various sectors such as human medicine, food handling and processing, industrial, domestic, veterinary settings and institutional settings are tested at MIS.
We receive various product samples for testing such as –
- Hygienic hand rub
- Handwash
- Instrument disinfectants
- Surface disinfectants
- Textile disinfectants
Disinfectant and Sanitizer Test Methods Performed
Microbe Investigations Switzerland offers a comprehensive range of efficacy testing for surface disinfectants and antiseptics, including antibacterial, antiviral and fungicidal/yeasticidal tests. These standards include European standards (EN), ISO standards, ASTM standards and AOAC standards.
Antibacterial Test Methods for Disinfectants
EN Standards:
- EN 1040: 2005 – Quantitative (phase 1 suspension) test to evaluate the basic bactericidal activity of chemical disinfectants and antiseptics
- EN 14347: 2005 – Quantitative (phase 1 suspension) test for basic sporicidal activity of chemical disinfectants and antiseptics
- EN 13727: 2012 + A2:2015 – Quantitative (phase 2 step 1 suspension) test for bactericidal activity of chemical disinfectants intended for medical area
- EN 14348: 2005 – Quantitative (phase 2 step 1 suspension) test for mycobactericidal or tuberculocidal efficacy testing of chemical disinfectants and antiseptics intended for medical area
- EN 17126: 2018 – Quantitative (phase 2 step 1 suspension) test for sporicidal efficacy testing of chemical disinfectants and antiseptics intended for medical area
- EN 1656: 2019 – Quantitative (phase 2 step 1 suspension) test for bactericidal efficacy testing of chemical disinfectants and antiseptics intended for veterinary area
- EN 14204: 2012 – Quantitative (phase 2 step 1 suspension) test for mycobactericidal efficacy testing of chemical disinfectants and antiseptics intended for veterinary area
- EN 13623: 2020 – Quantitative (phase 2 step 1 suspension) test for bactericidal activity of disinfectants against Legionella pneumophila in aqueous systems
- EN 13704: 2018 – Quantitative (phase 2 step 1 suspension) test for sporicidal efficacy testing of chemical disinfectants used in food, industrial, domestic and institutional areas
- EN 1499: 2013 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of hygienic hand wash products used in the medical area
- EN 1500: 2013 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of hand rub products used in the medical area
- EN 12791: 2016 + A1: 2017 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of chemical disinfectants and antiseptics intended for surgical hand disinfection
- EN 14561: 2006 – Quantitative (phase 2 step 2 suspension) test for bactericidal efficacy testing of chemical disinfectants and antiseptics intended for instrument disinfection in medical areas
- EN 14563: 2008 – Quantitative (phase 2 step 2 suspension) test for mycobactericidal or tuberculocidal efficacy testing of chemical disinfectants intended for instrument disinfection in the medical area
- EN 16615: 2015 – Quantitative (phase 2 step 2 suspension) test for assessing bactericidal and yeasticidal activity of disinfectant wipes used in medical areas
- EN 16616: 2015 – Quantitative (phase 2 step 2 suspension) test for chemical-thermal textile disinfection used in media areas
- EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
- EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in medical areas
- EN 14349: 2012 – Quantitative (phase 2 step 2 suspension) test for Evaluation of the bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area
- EN 16437: 2014 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area
- EN 13697: 2015 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas
ASTM Standards:
- ASTM E2799: 20212 – Determination of disinfectant efficacy against Pseudomonas aeruginosa biofilm using the MBEC Assay
- ASTM E2871: 2021 – Determination of disinfectant efficacy against biofilm grown in the CDC biofilm reactor using the single-tube method
- ASTM E2274: 2016 – Determination of microbicidal efficacy of laundry sanitizers and disinfectants
- ASTM E2406: 2016 – Evaluation of bactericidal efficacy of laundry sanitizers and disinfectants for use in high-efficiency washing operations
- ASTM E2362: 2022 – Evaluation of antimicrobial efficacy of pre-saturated or impregnated towelettes for hard surface disinfection
- ASTM E1054: 2022 – Test method for evaluation of inactivators of antimicrobial agents
- ASTM E1153: 2022 – Evaluating the efficacy of sanitizers intended for inanimate, hard, non-porous, non-food contact surfaces
- ASTM E1174: 2021 – Test method for healthcare personnel handwash formulations
- ASTM E2276: 2019 – Test method for bactericidal activity of hygienic handwash and hand rub agents
AOAC Standards:
- AOAC 955.15 – AOAC Use Dilution Method for disinfectants against Staphylococcus aureus
- AOAC 964.02 – AOAC Use Dilution Method for disinfectants against Pseudomonas aeruginosa
- AOAC 955.16 – Chlorine equivalency method for sanitizers and disinfectant products
- AOAC 960.06 – Determination of germicidal and detergent sanitizing action of disinfectants
- AOAC 965.12 – Evaluation of the tuberculocidal activity of disinfectants
- AOAC 965.13 – Test method for swimming pool water disinfectants
- AOAC 966.04 – Test method for evaluating sporicidal activity of disinfectants
- AOAC 991.47 – Evaluating the bactericidal activity of disinfectants against Salmonella choleraesuis
EPA Standards:
- EPA MLB SOP MB 40 – Evaluating the efficacy of antimicrobial surface coatings
- EPA #01 01A – Determining residual sanitizing efficacy of dried chemical residues on hard non-porous surface
- EPA MLB SOP MB 41 – Determining antimicrobial activity of hard, non-porous copper-containing surface products
PAS Standards:
- PAS 2424 – Evaluating residual bactericidal and/or yeasticidal) efficacy of liquid chemical disinfectants
Antiviral Test Methods for Disinfectants Efficacy Testing
EN Standards:
- EN 17914: 2022 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of chemical disinfectants and antiseptics in food, industrial, domestic and institutional areas
- EN 17915: 2022 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of chemical disinfectants on hard non-porous surfaces in food, industrial, domestic and institutional areas
- EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
- EN 14476: 2013 + A2: 2019 – Quantitative (phase 2 step 1 suspension) test for the virucidal activity of disinfectant or antiseptics used in medical areas
- EN 14675: 2015 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of disinfectant or antiseptics used in veterinary areas
- EN 13610: 2002 – Quantitative (phase 2 step 1 suspension) test for virucidal activity of disinfectant against bacteriophages used in food, industrial and domestic areas
- EN 16777: 2018 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of disinfectant used in the medical area
- EN 17111: 2018 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of medical instrument disinfectants
- EN 17122: 2019 – Quantitative (phase 2 step 2 suspension) test for virucidal activity of chemical disinfectants or antiseptics used in veterinary areas
ASTM Standards:
- ASTM E1053: 2020 – Assessing virucidal activity of chemicals used for disinfection of inanimate, non-porous environmental surfaces
- ASTM E1838: 2017 – Evaluating virus eliminating efficacy of hygienic handwash and hand rub agent
Fungicidal/Yeasticidal Test Methods For Disinfectants
- EN 1275: 2005 – Quantitative (phase 1 suspension) test for evaluating basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 13624: 2013 – Quantitative (phase 2 step 1 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 1657: 2016 – Quantitative (phase 2 step 1 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for veterinary area
- EN 1650: 2019 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants and antiseptics intended for medical areas
- EN 16438: 2014 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity of chemical disinfectants and antiseptics intended for veterinary area
- EN 14562: 2006 – Quantitative (phase 2 step 2 suspension) test for fungicidal or yeasticidal activity for instruments disinfectants used in the medical area
- EN 13697: 2015 + A1: 2019 – Quantitative (phase 2 step 2 suspension) test for bactericidal and/or fungicidal activity of chemical disinfectants and antiseptics intended for food, industrial, domestic and institutional areas
- EN 17272: 2022 – Quantitative (phase 2 step 2 suspension) test for microbicidal activity of automated airborne room disinfection processes
- EN 17387: 2021 – Quantitative (phase 2 step 2 suspension) test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in medical areas
PAS Standards
- PAS 2424 – Test method for evaluating residual bactericidal and/or yeasticidal efficacy of liquid chemical disinfectants.
To learn more about disinfectant testing, please check out our blog, “The Ultimate Guide to disinfectant testing.”
Variables to consider for a disinfectant qualification
When conducting a disinfectant qualification, several key variables must be considered to ensure accurate and effective testing. These include:
- Following the regulatory guidelines to ensure standardized testing
- Testing both environmental isolates and reference strains to evaluate real-world and benchmark efficacy.
- Testing various types of biocides to assess broad-spectrum activity, including sporicidal efficacy.
- Conducting the disinfectant tests at relevant temperatures to reflect real-world conditions.
- Evaluating the disinfectant performance on different materials (e.g., stainless steel, glass).
- Testing disinfectants at varying contact times to assess microbial kill rates.
Using organic material to simulate real-life contamination and its impact on efficacy.
Test microorganisms used for antimicrobial testing at MIS
Bacterial strains
- Staphylococcus aureus (ATCC 6538P)
- Klebsiella pneumoniae (ATCC 4352)
- Escherichia coli (ATCC 8739)
- Legionella spp. (ATCC 700511)
- Salmonella enterica (ATCC 10708)
- Pseudomonas aeruginosa (ATCC 15442)
- Enterococcus hirae (ATCC 10541)
- MRSA (ATCC 3391)
Viral strains
- Human adenoviruses (ATCC VR-5)
- Vaccinia virus (ATCC VR-1549)
- Betacoronavirus 1 (ATCC VR-1558)
- Human coronavirus (ATCC VR-740)
- Influenza A virus (H3N2) (ATCC VR-1679)
- Influenza A virus (H1N1) (ATCC VR-1469)
- Feline calicivirus (FCV) F-9 (US Patent strain)
- Poliovirus Type 1 (Sabin) (LSc-2ab)
- Murine norovirus (MNV) (S99)
Fungal and yeast strains
- Aspergillus niger (ATCC 16888)
- Penicillium funiculosum (ATCC 11797)
- Chaetomium globosum (ATCC 6205)
- Aureobasidium pullulans (ATCC 15233)
- Candida auris (MYA5002)
- Candida albicans (MTCC-3017)
Why trust MIS with your Disinfectant Product Testing?
At MIS, we utilize advanced technologies to evaluate the effectiveness of disinfectants and antiseptics. Our team of expert microbiologists meticulously oversees testing processes to ensure precision and accuracy. We specialize in designing and executing both qualitative and quantitative tests tailored to meet the specific needs of our clients. Our commitment at MIS is to fully understand your testing requirements and assess your products against all essential quality criteria within a set timeframe. For comprehensive disinfectant efficacy testing of your products, contact us today.
FAQs on Disinfectant Testing Laboratory
The efficacy of a disinfectant is tested in a disinfectant testing laboratory using standardized methods typically involving exposing contaminated surfaces to a known concentration of disinfectant for a specific contact time and measuring the reduction in the number of viable microorganisms.
The length of exposure or contact time, is critical because insufficient contact time may not effectively kill or inactivate all microorganisms on the surface, reducing the overall efficacy of the disinfectant.
The label on the product or the manufacturer’s instructions will indicate what pathogens the disinfectants will be effective against and the required contact time for effective disinfection.
Disinfectants are used to kill or inactivate microorganisms on inanimate surfaces, while antiseptics are applied to living tissue to inhibit microbial growth. In disinfectant testing, the focus is on evaluating the efficacy of products on inanimate objects, ensuring they meet safety and regulatory standards for environmental hygiene.
Disinfectants should be tested periodically, often annually or whenever there are changes in formulation, application procedures or regulations.
Yes, disinfectant testing can be customized to meet specific application needs, including testing against particular microorganisms or under specific environmental conditions.
Disinfectant testing laboratories commonly test surface disinfectants, instrument disinfectants, textile disinfectants, hygienic handwashes and hand rubs.
Key parameters evaluated during disinfectant testing include microbial reduction and testing conditions like contact time, concentration, temperature and the presence of organic matter.
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✓ Completed test order form (TOS)
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✓ FAST TRACK Program with less Turnaround Time