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AOAC 966.04
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Quick understanding of the test
AOAC 966.04 - Evaluation of Sporicidal Activity of Disinfectants
Application
Mandatory test strains
- Bacillus subtilis
- Clostridium sporogenes
- Test carriers are inoculated with spore suspensions and exposed to the test product for specific contact times.
- Treated carriers are transferred to a recovery medium, followed by incubation to qualitatively assess growth.
- Offers a standardized approach to evaluate the sporicidal activity, ensuring consistent and reliable results.
- Critical for compliance set by regulatory bodies like EPA (Environmental Protection Agency).
Turnaround Time
Passing criteria
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Abstract
AOAC 966.04 provides a test method to evaluate the efficacy of water-soluble powders and non-volatile liquid products for their sporicidal action.
AOAC 966.04 serves as the standard testing guideline for the registration of disinfectants that claim to have sporicidal efficacy as per regulatory requirements set by the US Environmental Protection Agency (EPA) and the US Food and Drug Administration (FDA).
AOAC 966.04 Test Method
- Peni cylinders and silk sutures are used as test surfaces (carriers) to perform the test.
- Each carrier is individually inoculated with spore suspension of Bacillus subtilis and Clostridium sporogenes. For AOAC sporicidal tests, inoculum size should be adjusted between 1.0ร105 to 1.0ร106. spores/ carrier.
- In the next step, carriers contaminated with test microorganisms are exposed to test products for specific contact times.
- At the end of contact time, treated carriers are immediately transferred to a recovery medium enriched with neutralizing compounds.
- Recovery medium is incubated for a period of 21 days. This incubation period allows for a comprehensive qualitative assessment of any potential growth that may still be present after disinfectant treatment.
Importance of AOAC 966.04 Test
Clostridium difficile and Bacillus subtilis are considered one of the major contributors for spreading infections and disease outbreaks within hospital settings. Both bacteria can form endospores, allowing them to endure harsh conditions and persist for extended periods. As a part of robust hygiene practices, disinfectants possessing sporicidal action should be employed to effectively eliminate these endospore-forming microbes.
Disinfectants labeled with sporicidal claims are registered and approved by regulatory bodies like the U.S. Environmental Protection Agency (EPA) and the U.S. Food and Drug Administration (FDA). AOAC 966.04 method is designed for compliance reporting of sporicidal disinfectants under EPA and FDA regulatory authorities.
Conclusion
At MIS, we offer an extensive portfolio to test sporicidal efficacy of a diverse range of disinfectant products.
Our team comprises highly skilled microbiologists who possess in-depth knowledge and experience in AOAC testing analysis.
Under our AOAC testing portfolio, we also perform AOAC 965.12 test for tuberculocidal activity of disinfectants.
To get a quote on AOAC 966.04 testing, talk to our experts today.
Frequently Asked Questions
DR. Martinoz Scholtz
AOAC 966.04 is a sporicidal activity test designed for evaluating efficacy of water-soluble powders and non-volatile liquid products. AOAC 966.04 is recommended by regulatory authorities FDA and EPA as part of the registration process of sporicidal disinfectants products.
AOAC 966.04 test is applicable to water-soluble powders and non-volatile liquid products intended for disinfection of hard surfaces.
AOAC 966.04 test takes 4-5 weeks to complete.
We test for AOAC 966.04 using the following bacterial strains: Bacillus subtilis and Clostridium sporogenes.
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