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BS EN 14675:2015
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BS EN 14675:2015 - Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area
Application
- Bovine enteroviruses (ECBO).
- The disinfectant sample is added to a solution of viral suspension and interfering substance and allowed to interact for a specified contact time. The reaction is stopped using a neutralizing solution, and viral infectivity is measured using the TCID50 assay.
- Confirms product efficacy in virus control in veterinary settings.
- Offers a consistent approach for evaluating virucidal activity.
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Abstract
EN 14675 is a phase 2, step 1 suspension test which determines the virucidal activity of chemical disinfectants and antiseptics intended for use in veterinary areas. According to EN 14675, the test must be conducted at a concentration of 80 percent or less, as adding organisms and interfering substances also results in some dilution.
EN 14675 test is applicable for products used in the following veterinary areas/situations –
- breeding, husbandry, production,
- transport and disposal of all animals except when in the food chain following death and entry to the processing industry.
EN 14675 Test Conditions & Requirements
- Test microorganisms used – Bovine enteroviruses (ECBO)
- Test temperature – 10 °C
- Contact time – 30 minutes
- Interfering substance (low-level soiling) – 3.0 g/l bovine albumin
- Interfering substance (high-level soiling) – 3.0 g/l bovine albumin plus 10g/l yeast extract
- Log reduction – For a product to pass virucidal efficacy criteria, it must achieve 4 log virus inactivation
Control test
Control tests are critical to ensure the accuracy and validity of the EN 14675 evaluation. These include:
- Cytotoxicity Control: Determines that test results are not affected by the cytotoxic effects of the disinfectant on the cell cultures.
- Interference Control: It ensures that the disinfectant’s action is virucidal and is not affected by the presence of interfering substances.
- Neutralization Control: It ensures that the disinfectant’s virucidal activity is effectively neutralized during the neutralization step.
- Reference control – A reference reagent (e.g., formalin) is used instead of the test product against test virus to ensure virucidal efficacy of the product sample.
EN 14675 Test Method
- Test product is added to viral suspension in the presence of an interfering substance.
- Mixture is allowed to interact for a specific duration of time.
- After the end of contact period, an aliquot of test mixture is transferred to a neutralizing solution (ice-cold diluent) to suppress virucidal activity.
- Neutralizing wash out media is serially diluted and viral infectivity is measured by using TCID50 assay.
- After an incubation period of 7 days, cells are examined for CPE (cytopathic effect).
Determination of Results
The results of the BS EN 14675 test are expressed as the logarithmic reduction of viral infectivity. A reduction of at least 4 logs is considered sufficient for the product to pass the test.
Importance of EN 14675 Test
Choosing a right and effective disinfectant is essential to maintain proper hygiene and germ free environment in veterinary facilities. In such environments, efficacy of disinfectants is frequently impacted by heavy soiled surfaces coupled with other hygiene factors.
EN 14675 stimulates practical conditions to determine virucidal effectiveness of disinfectant products before real use. Compliance with this standard supports regulatory alignment in Europe, offering manufacturers a structured approach to validating product claims.
Conclusion
Ensure your veterinary disinfectants meet the highest virucidal efficacy standards with EN 14675 testing. Our state-of-the-art antimicrobial lab provides precise and reliable testing to help you achieve regulatory compliance and validate your product claims. Partner with us to ensure your disinfectants are proven effective in real-world veterinary applications.
To get a quote on EN 14675 or any related test method, talk to our experts today.
Frequently Asked Questions
DR. Martinoz Scholtz
EN 14675 is a quantitative suspension test which evaluates the virucidal activity of chemical disinfectants and antiseptics to be used in veterinary areas for disinfection.
EN 14675 test is applicable for chemical disinfectants and antiseptics intended for use in veterinary areas.
EN 14675 test takes 4-5 weeks to complete.
At Microbe Investigations, we test for EN 14675 test using the following microbial strains: Bovine enteroviruses (ECBO).
The EN 14675 test standard requires a test temperature of 10°C ± 1°C and a contact time of 30 min ± 10 s with Bovine enterovirus Type 1 (ECBO) as the test organism.
EN 14675 is for veterinary disinfectants, while EN 14476 is intended to validate the efficacy of medical-area disinfectants.
Temperature impacts the efficacy and reaction rate of the disinfectant.
Phase 1 tests assess the basic antimicrobial properties of a disinfectant in a suspension.Whereas, Phase 2 tests evaluate its efficacy under real-world conditions, including the presence of interfering substances ( Phase 2 Step 1) and on contaminated surfaces ( Phase 2 Step 2).
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