BS EN 14675:2015

Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area

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Quick understanding about test

BS EN 14675:2015 - Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area

EN 14675 (phase 2, step 1 suspension test) evaluates the virucidal activity of chemical disinfectants and antiseptics intended for use in veterinary areas.
This test is applicable to disinfectants used in veterinary environments, including breeding, husbandry, production, and more.
Turnaround Time
The EN 14675 test requires approximately 4-5 weeks to complete.
The viral infectivity is measured using the TCID50 assay, and the results are determined by observing cytopathic effects (CPE) after a 7-day incubation.
For a disinfectant to pass, it must achieve at least 4-log reduction in virus infectivity.

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Abstract

EN 14675 is a phase 2, step 1 suspension test which determines the virucidal activity of chemical disinfectants and antiseptics intended for use in veterinary areas. According to EN 14675, the test must be conducted at a concentration of 80 percent or less, as adding organisms and interfering substances also results in some dilution.

EN 14675 test is applicable for products used in the following veterinary areas/situations –

  • breeding, husbandry, production, 
  • transport and disposal of all animals except when in the food chain following death and entry to the processing industry.

EN 14675 Test Conditions & Requirements

  • Test microorganisms used – Bovine enteroviruses (ECBO)
  • Test temperature  – 10 °C
  • Contact time –  30 minutes
  • Interfering substance (low-level soiling)  – 3.0 g/l bovine albumin
  • Interfering substance (high-level soiling) – 3.0 g/l bovine albumin plus 10g/l yeast extract
  • Log reduction –  For a product to pass virucidal  efficacy criteria, it must achieve 4 log virus inactivation

Importance of EN 14675 Test

Choosing the right and effective disinfectant is essential to maintain proper hygiene and germ-free environment in veterinary facilities.  In such environments, the efficacy of disinfectants is frequently impacted by heavily soiled surfaces coupled with other hygiene factors. 

EN 14675 standard stimulates practical conditions to determine virucidal effectiveness of disinfectant products before real use.

Conclusion

At MIS, EN 14675 testing is conducted by experienced virologists and research experts. To provide comprehensive assessment, our experts design and implement testing methods for antiviral evaluation which are most widely accepted and acknowledged by regulatory bodies.

With a highly equipped world-class laboratory, we are providing reliable and accurate results. 

MIS also offers EN 14476 test method for evaluation of the virucidal activity of disinfectants used in areas of human medicine.Need EN 14675 testing for your products or other related queries?  You can connect with our experts here.

Frequently Asked Questions

DR. Martinoz Scholtz

MIS Labs
1. What is EN 14675 test?

EN 14675 is a quantitative suspension test which evaluates the virucidal activity of chemical disinfectants and antiseptics to be used in veterinary areas for disinfection.

2. What are the Products that can be tested with EN 14675 test?

EN 14675 test is applicable for chemical disinfectants and antiseptics intended for use in veterinary areas.

3. What is the Turnaround Time for EN 14675 test?

EN 14675 test takes 4-5 weeks to complete.

4. On how many viruses can your EN 14675 testing lab perform tests on?

At Microbe Investigations, we test for EN 14675 test using the following microbial strains: Bovine enteroviruses (ECBO).

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