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ASTM E2755 Test

Determining the Bacteria-Eliminating Effectiveness of Healthcare Personnel Hand Rub Formulations Using Hands of Adults

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ASTM E2755: Test Method for Antibacterial Healthcare Personnel Hand Rub Formulations

ASTM E2755 provides a standardized test method to assess the antimicrobial efficacy of alcohol-based handrubs.

Given the importance of preventing infections within healthcare environments, this test method helps to ensure that hand sanitizers meet rigorous efficacy standards.

Test Conditions & Requirements of ASTM E2755

Mandatory test microorganisms – Serratia marcescens or Staphylococcus aureus (ATCC 6538P)

ASTM E2755 Test Method

  • The test is performed on the hands of volunteers who have no visible signs of damage and haven’t used any antimicrobial product for at least one week before the test
  • After an initial hand cleaning, the hands of participants are exposed to a specific test microorganism, and the starting baseline count of microorganisms is determined. The antibacterial activity is assessed following either a single application or multiple consecutive contamination/application cycles within a single day.
  • The antibacterial efficacy is determined by comparing the number of test bacteria present on the hands after the product use to the viable bacteria found after using a reference control.

Importance of ASTM E 2755

Hand hygiene is a fundamental aspect of preventing infections in healthcare environments, and the ASTM E2755 standard plays a crucial role in ensuring its effectiveness.

Pathogens can be transmitted via the hands of healthcare workers, making effective hand hygiene practices critical for patient safety. Alcohol-based handrubs are an effective and convenient way to ensure hand hygiene, provided they are effective against a wide range of pathogens.

The ASTM E2755 test method is a crucial tool in ensuring the effectiveness of alcohol-based handrubs in healthcare settings. By adhering to this standard, manufacturers and healthcare providers can play a vital role in preventing Hospital-acquired infections (HAI) and protecting patient and healthcare worker safety.

Why choose MIS for ASTM E2755 Testing?

  • Expertise: MIS has a team of experts who have hands-on experience in microbial testing.
  • Global reach: With facilities around the world, MIS can serve clients from various regions, ensuring their products meet global standards.
  • Customized solutions: Understanding that every product is unique, MIS offers tailored testing services to address the specific needs of each client.

If you want to know more about ASTM E2755 testing regarding test duration, test quote or test result interpretation feel free to contact our experts.


The ASTM E2755 is performed to assess how well hand rub formulations used by healthcare personnel are at removing transient bacterial flora from the hands.

ASTM E 2755 can test hand rubs, hygienic hand rubs, hand sanitizers, or hand antiseptics intended to be used in healthcare settings.

It can take 2-3 weeks to complete ASTM E 2755.

At Microbe Investigations, we test for ASTM E 2755 using the following microbial strains: Serratia marcescens or Staphylococcus aureus (ATCC 6538P). Additional stains can be added on the customer’s request.

Contact us for more information

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