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ASTM D4783
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ASTM D4783 - Assessing Resistance of Adhesives Preparations to Microbial attack in container
- Bacteria: Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus subtilis, Proteus vulgaris
- Fungi: Aspergillus niger, Aspergillus flavus, Penicillium pinophilum
- Yeast: Candida tropicalis, Candida pseudotropicalis, Kluyveromyces fragilis
- Adhesive samples are inoculated with microbial strains and incubated at controlled temperatures. Microbial growth is monitored on streak plates at specific intervals. If sterility is maintained after four challenges, the sample is considered resistant. Know more
- Evaluates adhesive durability in microbe-exposed environments.
- Ensures uniform quality assurance across all product batches.
- Positive Growth: Light, Medium, or Heavy microbial growth indicates failure.
- Negative Growth: The absence of microbial growth confirms resistance.
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Abstract
ASTM D 4783 Test Conditions & Requirement
Test conditions | |
Test organisms Bacteria | Pseudomonas fluorescens (ATCC 9721) Pseudomonas aeruginosa (ATCC 10145) Bacillus subtilis (ATCC 6984) Proteus vulgaris (ATCC 9920) |
Fungus | Aspergillus niger (ATCC 9642 ) Aspergillus flavus (ATCC 9643) Penicillium pinophilum (ATCC 9644) |
Yeast | Candida tropicalis ( ATCC 750) Candida pseudotropicalis (ATCC 4135 ) Kluyveromyces fragilis (ATCC 8554) |
Challenge and Sterility checks (Hrs and Days) | 4, 24, 48, 72 hrs and at 7 days |
Incubation Temperature (°C) | Bacteria and Yeast: 30-35 ; Fungus: 20-25 |
Inoculum size | Bacteria : 1.0 x 10^8 bacteria/mL |
Yeast: 1.0 x 10^7 yeasts/mL | |
Fungus: 1 x 10^6 spores/mL | |
Passing Criteria (Growth Rate) | Light: Positive |
Medium: Positive | |
Heavy: Positive | |
Absence of growth: Negative | |
ASTM D 4783 Test Method
- The adhesive specimen is challenged by inoculating a bacteria, yeast, or fungi culture, which may be a single species or a mixed culture of several species.
- The inoculated adhesive specimen is stored at a controlled temperature for definite intervals or days when cultures (streak plates) are made at preset intervals.
- At any point in the series of challenges, the test is discontinued if the inoculated specimen shows microbial growth on the streak plates made during the week following the challenge (indicating that it has not returned to sterility). The sample is reported as not resistant to attack in the container by the species or combination of species used as the inoculum.
- If the culture shows no growth, the test is repeated with up to four challenges. If the specimen tests out as sterile following the fourth challenge, it is reported to be resistant to attack in the container by the specific strains or combination of species of bacteria, fungi, or yeast used in inoculum.
- The time necessary to kill is determined by noting the earliest streak plate to read sterile.
Importance of ASTM D 4783 Test
- The ASTM D 4783 Test demonstrates whether an adhesive preparation is sufficiently protected with biocide to resist bacteria, yeast, and fungi attacks during storage.
- ASTM D 4783 is patterned after methods used by biological laboratories serving the adhesive industry from biodeterioration of adhesives.
- This test method is advantageous when tested against wild-type microorganisms isolated from contaminated adhesives to determine the amount and type of biocide necessary to kill or inhibit the growth of the contaminants.
Frequently Asked Questions
DR. Martinoz Scholtz
ISO 22196 certification gives a confirmation of the antibacterial efficacy of plastic surfaces, and once products pass for this standard, they satisfy international hygiene requirements, which boosts customer confidence and aids in regulatory compliance.
A good result typically shows a significant reduction in bacterial growth, often measured as a logarithmic reduction. Higher the log reduction, better the efficacy.
Contact time, surface topography and environmental conditions like temperature and humidity can impact the effectiveness of the antibacterial treatment.
The test is specific to plastic and non-porous surfaces. It does not apply to porous materials or environments where multiple types of microorganisms may be present.
Yes, JIS Z 2801 is an alternative test used to evaluate antibacterial activity on different plastic materials.
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