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ASTM D4783
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Quick understanding about test
ASTM D4783 - Assessing Resistance of Adhesives Preparations to Microbial attack in container
- Bacteria: Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus subtilis, Proteus vulgaris
- Fungi: Aspergillus niger, Aspergillus flavus, Penicillium pinophilum
- Yeast: Candida tropicalis, Candida pseudotropicalis, Kluyveromyces fragilis
- Adhesive samples are inoculated with microbial strains and incubated at controlled temperatures. Microbial growth is monitored on streak plates at specific intervals. If sterility is maintained after four challenges, the sample is considered resistant. Know more
- Evaluates adhesive durability in microbe-exposed environments.
- Ensures uniform quality assurance across all product batches.
- Positive Growth: Light, Medium, or Heavy microbial growth indicates failure.
- Negative Growth: The absence of microbial growth confirms resistance.
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Abstract
ASTM D 4783 Test Conditions & Requirement
ASTM D 4783 Test Method
- The adhesive specimen is challenged by inoculating a bacteria, yeast, or fungi culture, which may be a single species or a mixed culture of several species.
- The inoculated adhesive specimen is stored at a controlled temperature for definite intervals or days when cultures (streak plates) are made at preset intervals.
- At any point in the series of challenges, the test is discontinued if the inoculated specimen shows microbial growth on the streak plates made during the week following the challenge (indicating that it has not returned to sterility). The sample is reported as not resistant to attack in the container by the species or combination of species used as the inoculum.
- If the culture shows no growth, the test is repeated with up to four challenges. If the specimen tests out as sterile following the fourth challenge, it is reported to be resistant to attack in the container by the specific strains or combination of species of bacteria, fungi, or yeast used in inoculum.
- The time necessary to kill is determined by noting the earliest streak plate to read sterile.
Importance of ASTM D 4783 Test
Liquid adhesives are widely used across industries, and their performance depends on stability during storage and use. If left unprotected, they can easily spoil due to microbial growth, leading to foul odor, discoloration, and loss of bonding strength. The ASTM D4783 test method evaluates how well adhesives resist bacteria, yeast, and fungi. By simulating microbial challenges and measuring whether the adhesive returns to sterility, it helps manufacturers evaluate and optimize biocide systems, choose appropriate preservatives, satisfy regulatory or quality-control requirements, and ensure that products delivered to end users maintain their intended performance and shelf life.
ASTM D 4783 Testing Services Available at MIS
Discover the full spectrum of our comprehensive antimicrobial testing portfolio at Microbe Investigations Switzerland (MIS). Our services strictly adhere to international standards, ensuring precise and reliable results. With our team of experts and a certified advanced laboratory setup, we are committed to providing fast turnaround times for all your testing needs, including biocide adhesive samples following ASTM D 4783 guidelines.
For more detailed information on our testing services, turnaround times, or any other inquiries, contact us today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM D4783 test is performed to determine the resistance of liquid adhesive preparations to microbial attack in the container. This method involves exposing adhesive specimens to specific cultures of bacteria, yeast, or fungi to assess their ability to withstand contamination and degradation over time.
This test is applied to liquid adhesive preparations.
ASTM D4783 test takes 2-6 weeks to complete.
At Microbe Investigations, we test for ASTM D4783 using the following microbial strains: Pseudomonas fluorescens (ATCC 9721), Pseudomonas aeruginosa (ATCC 10145), Bacillus subtilis (ATCC 6984), Proteus vulgaris (ATCC 9920), Candida tropicalis (ATCC 750), Candida pseudotropicalis (ATCC 4135), Kluyveromyces fragilis (ATCC 8554), Aspergillus niger (ATCC 9642), Aspergillus flavus (ATCC 9643), and Penicillium pinophilum (ATCC 9644)
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