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ASTM D4300 Test Method

Antifungal Test for Adhesive Films

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ASTM D 4300:2023 Standard guideline to test the ability of adhesive films to support or resist the growth of fungi

ASTM D 4300 standard describes the method to evaluate the resistance of adhesive films against fungal growth.

ASTM D 4300 standard is based on the physiological action of microbes of interest under specified conditions. Antifungal performance of tested adhesive films is determined by comparing the results to control samples exhibiting fungus-resistant properties.

Potato dextrose agar (PDA) and Mineral salts agar (MSA) are two agar substrates used in the experiment. PDA provides a complete medium for fungal growth, while mineral salt agar (MSA) is less conducive for fungal growth as it lacks carbohydrates in its nutrient composition.

The purpose of using PDA media for adhesive film testing is to assess how well the test sample resists the growth of fungi on the media surface and to repel a full growth of fungi on the adjacent agar surface. Whereas, MSA media is employed in adhesive film testing to resist the growth of fungi on its surface, minimizing the chance of misinterpreting fungal growth from the adhesive film as growth from the agar.

Test process of ASTM D4300

ASTM D4300 Test Method

  • Spores suspension is inoculated on PDA and MSA plates. Then, three adhesive-coated fiber-glass disks are placed on both media plates. In parallel, a control test is run by placing three uncoated Whatman No. 1 filter paper disks on the media surfaces. Using a sterile capillary dropping pipet, three drops of the spore suspension are inoculated on the surface of each disk on the test plates and the control plate.
  • Plates are sealed with parafilm to prevent drying out during incubation. Plates are held at 25 ± 0.5°C.
  • Plates with PDA media are examined at 3, 7, and 14 days for zone of inhibition (ZI) or overgrowth on the adhesive-coated disks.
  • For slow-growing species, the incubation period is extended to 21 days. While plates with MSA medium are examined at 7, 14, and 21 days.

Importance of ASTM D4300 Test

Antifungal adhesive films are thin, flexible sheets or coatings that incorporate antifungal agents to prevent or inhibit the growth of fungi on various surfaces. These films provide a protective barrier against fungal colonization, prevent biodegradation, and extend the lifespan of products.

ASTM D4300 stipulates the test procedure to ensure the effectiveness of antifungal adhesive films and improve optimal performance for their intended applications.

At MIS, we specialize in conducting antifungal testing for a wide range of materials including adhesive films, plastics, paints, coating, and textiles.

Our team comprises the experts who have hands-on experience to develop a framework for efficacy testing and execute the analytical procedures. We also offer bespoke solutions to meet the distinct requirements of each client’s product.

With our comprehensive testing services, our constant focus is to deliver outcomes of the highest quality in terms of accuracy, precision, and transparency, all within a defined time frame.

Among our array of antifungal testing, ASTM D G21 is one of the most frequently performed test methods in our labs.

To get a quote on the ASTM D 4300 test, talk to our experts here.


ASTM D 4300 test is designed to assess the ability of adhesive films to resist fungal growth when applied to intended areas of use.

ASTM D 4300 test is employed to determine the efficacy of adhesive films claiming to have antifungal properties.

ASTM D 4300 test takes 3- 4 weeks to complete.

At Microbe Investigations, we test for ASTM E2406 using the following microbial strains :
Aspergillus niger (ATCC 9642), Aureobasidium pullulans (ATCC 15233), Chaetomium globosum (ATCC 6205), Gliocladium virens (ATCC 9645) and Penicillium pinophilum (ATCC 9644).

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