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EPA MLB SOP MB-41
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Quick understanding of the test
EPA MLB SOP MB-41 - Standard Method for the Evaluation of Antimicrobial Activity of Hard, Non-porous Copper-containing Surface Products
Application
Mandatory test strains
- Staphylococcus aureus (ATCC #6538)
- Pseudomonas aeruginosa (ATCC #15442)
- Viruses
- Copper carriers and stainless steel control carriers are coated with antimicrobial chemicals.
- The carriers are exposed to the chemical treatments and abrasion for defined periods.
- After abrasion, the carriers are challenged with test organisms for a specified contact time.
- After contact time, the test specimen is neutralized using a neutralizing solution, and the surviving test organisms are recovered.
- Provides a realistic evaluation of product performance under typical usage conditions.
- Evaluates residual activity and long term effectiveness of the surface treatments.
Turnaround Time
Passing criteria
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Abstract
EPA MLB SOP MB-41 Test determines the efficacy of hard, non-porous Copper-containing surface products with antimicrobial activity against test bacteria. The method is used to assess the antimicrobial activity upon mechanical abrasion of surface coatings containing Copper, on hard, non-porous surfaces against prescribed microbes such as Staphylococcus aureus, Pseudomonas aeruginosa, and Viruses. However, the test can be adapted to other bacterial strains as well.
EPA MLB SOP MB-41 Test Method is divided into 2 parts
Part 1: Abrasion and chemical treatment
- Copper carriers and stainless steel control carriers of size 2.5 sq. cm. are brush-coated with antimicrobial chemicals and act as test specimens, with uncoated carriers acting as controls.
- The carriers are exposed to at least 3 different chemical treatments for any antimicrobial sample, each followed by an abrasion cycle.
- The carriers are exposed to the chemical treatments and abrasion 5 times a day, for 5 days a week, for 6 weeks continuously.
Part 2: Product efficacy
- After the completion of the treatment and abrasion, the carriers are challenged with a 20ฮผL mixture of the test organism and soil load for a contact time of 1-2 hours.
- After the contact time, the antimicrobial action of the test specimen is neutralised using a neutralising media, and the surviving test organism is recovered.
- The number of viable microorganisms recovered from the controls (uncoated and unabraded) and test specimens (coated and aberrated) is determined quantitatively.
- The number of viable microorganisms on treated and/or abraded carriers is compared to the untreated control carriers and the antimicrobial efficacy is determined by log reduction.
A minimum of 3 log reduction in microorganisms within 1-2 hrs indicates a residual claim for the antimicrobial surface coating.
Importance of the test
EPA MLB SOP MB-41 testing provides supporting efficacy data for the registration of hard, non-porous Copper-containing surface products used to supplement standard disinfectants to augment their antimicrobial activity. The chemical treatments and abrasions performed in the test are intended to represent normal wear & tear and multiple exposures to biocidal materials.
Conclsion
Here at MIS, we develop and optimize test protocols for EPA MLB SOP MB-41 which are complied with international standards and appropriate safety protocols are followed while testing surface coatings against human pathogenic microorganisms.
MIS also offers a test for evaluating the efficacy of antimicrobial surface coatings (EPA MLB SOP MB-40)
Frequently Asked Questions
DR. Martinoz Scholtz
It is a test that evaluates the efficacy of hard, non-porous Copper-containing surface products with antimicrobial activity against test bacteria. These products are used to supplement standard disinfectants, against test bacteria and viruses.
Hard, non-porous copper-containing surface products meant for indoor use and for continuous reduction of bacteria and viruses can be tested using this method. It is also applicable to solid Copper products, impregnated Copper products and Copper coated products.
The turnaround time is 4-5 weeks.
At present, Staphylococcus aureus, Pseudomonas aeruginosa and viruses can be tested. The test can be adapted for other microorganisms.
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