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ASTM E2276-03
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Quick understanding of the test
ASTM E2276-03 - Assessing the effectiveness of hygienic handwash and handrub products in eliminating bacteria using adult fingerpads
Application
- Serratia marcescens
- Escherichia coli
- Acinetobacter baumannii
- Staphylococcus aureus
- Staphylococcus epidermidis
- Adult participants with undamaged skin and no prior antimicrobial product use one week prior to testing are selected.
- Bacterial suspension is applied to finger pads and allowed to dry.
- Contaminated fingertips are exposed to the test product and control.
- Surviving microorganisms are eluted, and viable counts are determined.
- Validates the effectiveness of hand hygiene products using realistic conditions.
- Ensures products can effectively reduce harmful bacteria, crucial for preventing disease spread.
Turnaround Time
Passing criteria
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Abstract
ASTM E2276 outlines a test method for determining the efficacy of hygienic handwash and hand rub products in eliminating transient bacteria from hands.
The main goal of ASTM E2276-03 is to ensure that hand hygiene products meet certain efficacy criteria before they are marketed and used in healthcare settings, food service, and everyday life. Providing a standardized testing method, helps manufacturers improve their products and give consumers confidence in the hand hygiene products they use.
Test Conditions and Requirements
- Mandatory test microorganisms – Serratia marcescens, Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus, and Staphylococcus epidermidis
- Contact time – 10 seconds, no longer than 30 seconds
Test method
- This test method is carried out on a group of adult subjects who have given informed consent and whose hand skin is free of any visible damage. The subjects are required to avoid the use of any products containing antimicrobial agents for one week before the test.
- A specific amount of test suspension is applied on a demarcated area on each finger pad and then allowed to dry.
- The contaminated fingertips are then exposed to the control ( hard water) and test ( product sample) for the specified contact time.
- In the next step, surviving microorganisms from the fingerpads are eluted and the viable counts are determined.
- Reductions in the numbers of viable bacteria from the control and product samples are calculated and analyzed for antibacterial efficacy.
Importance of ASTM E2276 test
Adhering to ASTM E2276-03 is critical for several reasons:
- Public health – Ensuring the effectiveness of hand hygiene products directly impacts public health by reducing the spread of infectious diseases.
- Regulatory compliance – Manufacturers must comply with standards like ASTM E2276-03 to market their products as effective antimicrobial agents.
- Consumer confidence – Products tested according to recognized standards instill confidence among consumers and healthcare professionals about their efficacy.
Conclusion
ASTM E2276-03 provides a crucial framework for evaluating the effectiveness of hand hygiene products. Manufacturers, consumers, and healthcare professionals need to understand and value the importance of certified hand hygiene products in promoting health and preventing disease.
MIS is a global microbial testing laboratory specializing in the evaluation of disinfectant products, including handwash and hand rub agents. With state-of-the-art facilities and a team of experienced microbiologists, MIS ensures that products meet or exceed the rigorous requirements of standards like ASTM E2276-03.
Contact MIS today to schedule your product testing for ASTM E2276-03.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM E2276 outlines a test method for determining how effectively hygienic handwash and handrub products kill bacteria on the fingers of adult participants. It helps to ensure hand hygiene products should meet certain efficacy criteria before they are marketed.
ASTM E 2276 test is applicable for handwash and hand rub products claiming antibacterial properties.
ASTM E 2276 usually takes 2-3 weeks to complete.
At Microbe Investigations Switzerland, we test for ASTM E 2276 using the following microbial strains: Serratia marcescens, Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus, and Staphylococcus epidermidis. Additional stains can be added at customerโs request.
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