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ASTM D2574
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Quick understanding about test
ASTM D2574: 2016 - Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms
- Pseudomonas aeruginosa (ATCC 10145)
- Klebsiella aerogenes (ATCC 13048)
- Bacillus subtilis (ATCC 27328)
- The test involves preparing paint samples with and without biocides, inoculating them with specified microbial strains, and incubating them for up to 7 days. Samples are monitored for microbial growth and changes in physical properties.
- Ensures microbial resistance and product quality.
- Prevents product recalls and damage from microbial contamination.
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Abstract
ASTM D2574 test requirements
- Test conditions – The test is to be conducted at a constant temperature of 30ยฐC ยฑ 2ยฐC. All environmental parameters like humidity, exposure to light, and inoculation with microbes, are strictly controlled to simulate the storage conditions.
- Sample requirements – Biocide-treated and untreated (control) samples are required.
- Control Test – A control is run alongside treated samples to assess if the preservative in the test sample is providing required protection. All test paints must be properly labeled, including the concentration of biocide to ensure accurate analysis and reporting.
- Test organisms – Pseudomonas aeruginosa (ATCC 10145) and Enterobacter aerogenes (ATCC 13048) are used as standard test organisms. Additional strains, such as Bacillus subtilis (ATCC 27328), can be included upon client request.
ASTM D2574 test methodology
Samples are tested against the challenge organisms for 2 cycles.
Cycle 1:
- Each test sample is inoculated with 0.1 mL of test organisms.
- The inoculated samples are incubated and bacterial recovery is assessed at Day 1, 2, 5 and 7 by streaking onto agar plates.
- The agar plates are then incubated for one week.
- After 7 days, plates are evaluated for bacterial recovery.
Cycle 2:
- On Day 7, the test samples are re-inoculated with 1.0 mL of each test organism.
- Incubation followed by bacterial recovery is repeated the same as for cycle 1.
Efficacy rating scale
Rating | Description |
0 | No bacterial recovery. |
1 | Trace of contamination (1 to 9 colonies) |
2 | Light contamination (10 to 99 colonies). |
3 | Moderate contamination (>100 distinct colonies). |
4 | Heavy contamination (continuous and indistinguishable smears of colony growth) |
Importance of ASTM D2574 antimicrobial paints test
Paints stored in containers are at constant risk of microbial spoilage. Contamination not only affects product appearance and performance but can also lead to foul odors, reduced shelf life, and customer complaints.
ASTM D2574 plays a key role in ensuring the quality and stability of emulsion paints. This standard helps manufacturers confirm that their preservative systems are strong enough to protect the paint during storage and distribution. By verifying microbial resistance, ASTM D2574 not only safeguards product integrity but also reduces the risk of customer complaints, financial losses, and damage to brand reputation. Since it is an internationally recognized ASTM standard, it also provides credibility, supports regulatory compliance, and gives customers confidence in the durability and reliability of the paint.
Strengths of ASTM D2574 test standard
- In-depth assessment – The standard provides a comprehensive evaluation detailing how well a paint formulation can resist microbial contamination.
- Standardization – Itโs a globally accepted standard that ensures uniformity of testing methods in various laboratories and manufacturers.
- Objective analysis – The rating scale allows for an objective comparison between various paint samples and formulations.
- Flexibility – It allows the testing of multiple microbial strains and various concentrations of biocides.
Conclusion
At Microbe Investigations Switzerland, we deliver precise ASTM D2574 testing to verify your paints withstand microbial attack and maintain quality.
Besides ASTM D2574, We also provide ASTM D4783 testing to evaluate the resistance of adhesive preparations against bacteria, yeast, and fungi.
Partner with us to safeguard product quality and compliance โ contact our experts today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM D2574 is an antimicrobial efficacy test that determines the resistance of emulsion paints containing biocides from microbial deterioration.
The test is applicable for emulsion paints.
The test takes 2-3 weeks to complete.
At Microbe Investigations Switzerland we perform the test using Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Aspergillus niger (ATCC-16888), Candida albicans (MTCC 3017).
Industries involved in the manufacturing and distribution of paints, coatings, and emulsions widely use this standard.
This standard is specifically designed for emulsion paints and does not typically cover adhesives. However, similar testing protocols like ASTM D4783 can be applied to assess the microbial resistance of adhesive products.
No, this test method is intended for water-based (emulsion) paints and not solvent-based products.
The limitations include its scope of applicability as it applies only to emulsion paints.
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