AOAC 991.47

Evaluation of disinfectant sample for its bactericidal activity against Salmonella choleraesuis

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Quick understanding of the test

AOAC 991.47 - Evaluation of disinfectant sample for its bactericidal activity against Salmonella choleraesuis

AOAC 991.47 evaluates the bactericidal efficacy of disinfectants against Salmonella choleraesuis on hard surfaces.

Application

Applicable to water-soluble powders and non-volatile liquid products intended for disinfecting hard surfaces.

Mandatory test strains

  • The carriers are inoculated with the test organism and subsequently exposed to the test product for specified contact times.
  •  After treatment, carriers are transferred to a recovery medium containing neutralizing compounds.
  • The recovery medium is incubated to qualitatively assess any remaining microbial growth.

Benefits

Turnaround Time

It typically takes 4-5 weeks.
Results are expressed qualitatively as the presence or absence of microbial growth in the recovery medium after incubation.

Passing criteria

No microbial growth is observed in the recovery medium of all treated carriers.

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Abstract

AOAC 991.47 test lays down a standard guideline to test the efficacy of a given disinfectant sample against Salmonella choleraesuis on a hard surface carrier.

AOAC 991.47 is an important guideline that helps to verify the performance of the disinfectant sample against Salmonella choleraesuis, a highly pathogenic species of Salmonella that causes septicemic diseases. AOAC 991.47 thus serves as the standard testing guideline for registration of the disinfectants that claim to have bactericidal efficacy against Salmonella choleraesuis as per regulatory requirements set by the US Environmental Protection Agency (EPA) and US Food and Drug Administration (FDA).

AOAC 991.47 Test Method

  •   70 carriers are used for a single test sample. Each carrier is inoculated with the test organism.
  •   In the next step, 60 carriers contaminated with test microorganisms are exposed to the test product for specific contact times and the remaining 10 are kept as untreated controls.
  •   At the end of contact time, treated carriers are immediately transferred to a recovery medium enriched with neutralizing compounds.
  •   Recovery medium is then incubated for a comprehensive qualitative assessment of any potential growth that may still be present after disinfectant treatment.

Conclusion

At MIS, we offer an extensive portfolio to test the bactericidal efficacy of a diverse range of disinfectant products. 

Our team comprises highly skilled microbiologists who possess in-depth knowledge and experience in AOAC testing analysis.

Under our AOAC testing portfolio, we also perform an AOAC 965.12 test for the tuberculocidal activity of disinfectants.

To get a quote on AOAC 991.47 testing, talk to our experts today.

Frequently Asked Questions

DR. Martinoz Scholtz

MIS Labs
1. What is AOAC 991.47 test?

AOAC 991.47 is a carrier-based test that helps to validate the bactericidal performance of a disinfectant sample against Salmonella choleraesuis.

2. What are the Products that can be tested with AOAC 991.47 test?

AOAC 991.47 test is applicable to water-soluble powders and non-volatile liquid products intended for the disinfection of hard surfaces against Salmonella choleraesuis.

3. What is the Turnaround Time for AOAC 991.47 test?

AOAC 991.47 test takes 4-5 weeks to complete.

4. Which microbial strain is used to test AOAC 991.47 in the lab?

We test for AOAC 991.47 using Salmonella choleraesuis.

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