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AOAC 961.02 (2009)
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Quick understanding of the test
AOAC 961.02 (2009) - Efficacy test for germicidal spray products as disinfectants
Application
Mandatory test strains
- Staphylococcus aureus
- Pseudomonas aeruginosa
- Salmonella enterica
- Test carriers are inoculated with microbial inoculum and dried.
- The disinfectant spray is applied to the dried carriers, incubated for a specific contact period.
- Carriers are transferred to neutralizing solutions.
- Viable microorganisms are quantified by plating the neutralizing extract, ensuring complete disinfection is achieved.
- Provides a reproducible and standardized procedure to measure the germicidal activity of spray disinfectants.
- Ensures compliance with regulatory bodies like EPA for product efficacy.
Turnaround Time
Passing criteria
- To pass AOAC 961.02 test (60-carrier test), at least 59 out of 60 carriers must show no growth of the test organism to pass.
- For a 10-carrier test, the product should demonstrate complete disinfection.
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Abstract
AOAC 961.02, known as the Germicidal Spray Products Test, is a standardized method for evaluating the germicidal efficacy of spray disinfectants. This test is one of the EPA-recommended methods for registering antimicrobial sprays in the United States. This carrier-based test evaluates products under simulated real-world use conditions, especially on hard, non-porous surfaces that may be contaminated with dangerous pathogens like Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica.
AOAC 961.02 Test Method
- Microscope slides of size 18 x 36 mm ( referred as test carriers) are placed in petri plates.
- Microbial inoculum is spread onto test carriers and is allowed to dry out.
- Few inoculated test carriers are enumerated to know initial microbial concentration.
- Product samples are sprayed over remaining dried inoculated slides. These slides are further incubated for specific contact period time.
- At the end of contact period, slides are transferred to individual containers containing neutralizing solutions.
- Neutralizing extract is plated and incubated to determine viable microorganisms.
- To pass AOAC 961.02 test, there should be complete disinfection on test career surfaces.
Passing Criteria
- To pass AOAC 961.02 test (60-carrier test), at least 59 out of 60 carriers must show no growth of the test organism to pass.
- For a 10-carrier test, the product should demonstrate complete disinfection.
Importance of AOAC 961.02 Test
Certain microorganisms, such as Staphylococcus and Salmonella, can survive for extended periods on hard, non-porous surfaces and are known to cause serious infections. This makes it vital to ensure that disinfectants used against these pathogens are both effective and reliable. The AOAC 961.02 test plays a key role in validating this efficacy by simulating the real world scenarios using carrier surfaces for testing , providing scientifically backed data that supports both product claims and regulatory compliance. Moreover, passing AOAC 961.02 is a requirement for EPA registration, which is necessary to legally market your spray product as a disinfectant in the United States.
Conclusion
Microbe Investigations provides efficacy testing for germicidal disinfectant sprays products by using AOAC 961.02.
We design and perform testing protocols according to international standards and provide precise and accurate results to clients. While doing microbiological analysis of products, we ensure to include specified and emerging pathogens as test microorganisms.
Also, we also perform AOAC use dilution tests to measure the antimicrobial efficacy of liquid disinfectants on inanimate surfaces.
Need further assistance on AOAC 961.02 germicidal spray test, contact with our experts now.
Frequently Asked Questions
DR. Martinoz Scholtz
AOAC 961.02 standard is designed to assess germicidal efficacy of spray disinfectants intended to be used on inanimate, non-porous surfaces.
AOAC 961.02 germicidal spray test is applicable for disinfectant products which do not require dilution. Products that can be tested with AOAC 961.02 test include trigger sprays and aerosols disinfectants.
AOAC 961.02 test takes 1-2 weeks to complete.
At Microbe Investigations Switzerland, we test for AOAC 961.02 using the following bacterial and fungal strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Aspergillus niger (ATCC-16888), Candida albicans (MTCC 3017).
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