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JIS L 1922
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Determination of Antibacterial Activity of Antibacterial
Finished Products (Textiles)
Quick understanding of the test
JIS L 1922 – Determination of Antiviral Activity of Textile Products
Mandatory test strains
- Influenza A Viruses (H1N1 and H3N2)
- Feline Calicivirus
- Control (untreated) and test (treated) fabric samples are placed in sterilized vial containers.
- For control samples, SCDLP broth is added immediately after inoculation.
- To verify cell sensitivity and cytotoxicity, three control and three treated samples are agitated with 20 mL SCDLP, and 5 mL washout solution is transferred to test tubes.
- 50 μL virus suspension is added to the washout solution and are incubated at 25°C for 30 minutes.
- After 2 hours, 20 mL SCDLP is added to all vials and vortexed to extract residual virus particles.
- The washout suspension is diluted serially, and viral infectivity is measured using Plaque or TCID50 assay.
Benefits
- Assists in meeting industry and regulatory standards for viral claims
- It provides accurate antiviral evaluation.
Turnaround Time
Results
Passing criteria
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Abstract
JIS L 1922 is used to assess the antiviral efficacy of textiles and other porous materials. Textile products to be tested with JIS L 1922 include woven and knitted fabrics, fibres, yarns, and braids. As per antiviral analysis reports, viruses can survive on fabric materials and remain infectious for several days. Clothing contaminated with viruses acts as a reservoir of infection and poses a significant risk for transmission.
Antiviral textiles are textiles that either inhibit the growth of infectious virus particles or completely eliminate them. The JIS L 1922 test method helps check the efficacy of such antiviral textile materials against a set of test viruses.
JIS L 1922 is an identical test method to ISO 18184 with some modifications.
Organisms typically used in this method
- SARS CoV-2,
- Beta coronavirus (OC43),
- Human coronavirus (229E),
- Influenza A Viruses (H1N1 and H3N2),
- Poliovirus, Adenovirus,
- Murine Norovirus,
- Vaccinia virus,
- Feline Calicivirus.
- 6 untreated and 6 treated fabric samples are used for the control test.
- 3 control samples are used to determine the infectivity titer immediately after virus inoculation.
- 3 control and 3 treated samples are used to determine the infectious titre after a specified contact period.
- 3 control and 3 treated specimens are used for the verification of cytotoxic analysis.
JIS L 1922 Test Method
- Control (untreated) and test (treated) fabric samples are placed in the vial containers.
- All vial containers are further kept in the autoclave for sterilization for 15 minutes at a temperature of 121 °C and pressure of 15 psi.
- Pipette viral suspension to the vials containing specimens.
- Immediately after the virus inoculation, 20 ml of Soya Casein Digest Lecithin Polysorbate Broth (SCDLP) is added to 3 control samples.
- To verify the cell sensitivity and cytotoxic activity, 3 control and 3 treated samples are put in sterilized vial containers. Then, 20 ml of SCDLP is added into these vials and agitated for 5 seconds and 5 times. 5 ml of washout solution from each vial is now transferred into new test tubes. 50 μL of virus suspension of known titer is added to these test tubes and incubated for 30 minutes at 25°C. After the incubation, if cell damage is observed then washout solution needs to be modified or increased carefully.
- After the contacting period of 2 hrs, 20 ml of SCDLP is added to 3 control and 3 test samples. Vials are agitated using a vortex mixer to extract the remaining virus particles from the sample.
- Wash-out viral suspension is further used to make serial dilutions and the infectivity titre of virus is determined either using Plaque assay or TCID50 assay.
At Microbe Investigations (MIS), we focus on rapid and reliable antimicrobial testing of your products.
We understand the customer’s testing requirements and design the right protocols for the antimicrobial evaluation of product samples. Our research experts keep a track of daily test activities and interpret and compare the test results to established specifications and control limits.
For antiviral textile finishes, we perform JISL 1922 and ISO 18184 frequently in our testing facilities.
Get in touch with our experts today for more information on our antimicrobial testing services.
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DR. Martinoz Scholtz
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