ISO 22717 : 2015

Detection of Pseudomonas aeruginosa in cosmetic products Quick understanding of the test

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Quick understanding of the test

ISO 22717 : 2015 - Detection of Pseudomonas aeruginosa in cosmetic products Quick understanding of the test

ISO 22717 provides the guidelines for the detection of Pseudomonas aeruginosa contamination in cosmetic and personal care products.

Application

Applicable to a wide range of cosmetic and personal care products, both water-miscible and water-immiscible formulations.

Mandatory test strains

  • Initial suspension is prepared by dispersing the product sample into enrichment broth,  followed by incubation.
  • The incubated enrichment broth is streaked onto Cetrimide agar medium and re-incubated.
  • After incubation, plates are examined for the morphology of Pseudomonas aeruginosa colony, followed by identification tests.

Turnaround Time

The turnaround time is approximately one week.

The presence of Pseudomonas aeruginosa colonies indicates positive results, while no detectable contamination indicates negative results.

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Abstract

ISO 22717 specifies the test method to determine Pseudomonas aeruginosa contamination in cosmetic products.

ISO 22717 test has two steps. First step entails the detection of Pseudomonas aeruginosa in an enrichment broth. This subsequently leads to the 2nd step i.e. isolation of microbes on a selective agar medium.

ISO 22717 Test Method

  • Initial suspension is prepared by dispersing 1g or 1ml of product sample into enrichment broth.
  • Water-miscible products – 1 ml of product sample is dispersed in 9 ml of enrichment broth. 
  • Water-immiscible products – 1g of  product sample is premixed with a dispersing agent (e.g. polysorbate 80) and then dispersed in 9 ml of enrichment broth.
  • Filterable products – Filtration is performed for product samples through the membrane followed by initial suspension preparation.
  • This initial suspension is incubated at 32.5 ยฐC for 20h and up to a maximum period of  72h.
  • An aliquot of the incubated enrichment broth is streaked onto Cetrimide agar medium.
  • Streaked plates are incubated at 32.5 ยฐC for 24h and up to a maximum period of 48h. 
  • After incubation, petri plates are examined for the morphology of Pseudomonas aeruginosa colony. Additionally, biochemical tests are performed to confirm the presence of Pseudomonas aeruginosa.

Importance of ISO 22717 Test

Using cosmetic products contaminated with Pseudomonas aeruginosa can pose serious health risks to the  consumers. So it is vital to ensure the safety of cosmetics and personal care products prior to market release.

ISO 22717 standard specifies the testing methods to detect specific microorganisms, such as Pseudomonas aeruginosa in cosmetic products. This testing helps cosmetic companies to  understand the microbiological quality of  their product and enable them to make necessary improvements to safeguard consumer well-being.

Conclusion

Looking for ISO 22717 testing of your cosmetic products! MIS experts are here to help you.

With the help of our state-of-the-art facilities and cutting-edge technology, our team experts perform testing for a wide range of cosmetic products. Our team of industry specialists execute and closely monitor each step of the testing process to ensure the delivery of reliable and accurate results.

Furthermore, customers can also send their cosmetic product samples for ISO 22718 test.To get a quote on ISO 22717 testing, talk to our experts here.

Frequently Asked Questions

DR. Martinoz Scholtz

MIS Labs
1. What is ISO 22717 test?

ISO 22717 describes methods for microbiological risk analysis of cosmetic products concerning the presence of a specific microorganism, Pseudomonas aeruginosa.

2. What are the Products that can be tested with ISO 22717 test?

ISO 22717 testing is applicable to cosmetics and personal care products.

3. What is the Turnaround Time for ISO 22717 test?

ISO 22717 test method takes 1 week to complete.

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