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ISO 18415

Detection of specified & non-specified microorganisms in Cosmetic product

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ISO 18415 : 2017 – Detection of specified and non – specified Microorganisms in Cosmetic Products

ISO 18415 standard describes a test method to identify the presence of any specified microorganisms and anaerobic mesophilic non-specified microorganisms in cosmetic products.

ISO 18415 outlines guidelines for the microbiological risk analysis specifically for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Candida albicans.

ISO 18415 test involves the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination. This is further followed by isolation of microorganisms on non-selective agar media.

Test process of ISO 18415

ISO 18415 Test Method

  • To perform the test, cosmetic product samples are diluted in enrichment broth to prepare initial suspension.
      1. Water-miscible products – 1 ml of product sample is diluted in 9 ml of enrichment broth.
      2. Water-immiscible products –  Product sample is mixed with a dispersing agent (e.g. polysorbate 80) and then added to the enrichment  broth.
      3. Filterable products – Product sample is transferred onto the membrane in a filtration apparatus. After filtration, the membrane is washed using water or diluent. This membrane is transferred into enrichment broth to make initial suspension.
  • This initial suspension is incubated at 32.5 °C for at least 20 h.
  • After incubation, an aliquot of the incubated enrichment broth is streaked onto the surface of non-selective agar medium in petri dishes.
  • Petri dishes are inverted and  incubated at 32.5 °C for 48 h to 72 h. 
  • Identification of microorganisms is done using an identification kit. This kit provides instructions for inoculation, incubation, and reading. Final results of the identification methods are compared with the database for final analysis.

Importance of ISO 18415 Test

Microbially contaminated cosmetic products can cause skin allergies, irritations, and other other significant health concerns to the end users. Therefore, manufacturers/ companies need to ensure their products meet the necessary microbiological safety requirements.

ISO 18415 standard stipulates the test requirements and procedures to identify potential microbial contaminants in cosmetic products. This method plays a vital role to validate the safety of cosmetic products for the well being of customers.

Need assistance with ISO 18415 testing of your cosmetic products! MIS experts are here to help you.

We offer a comprehensive range of customizable solutions for detection of microorganisms in cosmetic and personal care products. Our cosmetic testing services encompasses the screening of products at various stages of development including ingredients, semi-manufactured and final products.

Additionally, we also test antimicrobial efficacy of preservatives used in cosmetic products. In our state-of-the-art testing facilities, we are dedicated to deliver test results of the highest quality and accuracy.

Furthermore, customers can also send their product samples for ISO 18416 testing.

To get a quote on the ISO 18415 test or any other queries related to our cosmetic testing services, reach out to our experts here.


ISO 18415 is a standard test method for identification of both specified and non – specified microbial contaminants in cosmetic products. The objective of the test is to ensure that cosmetic products are safe for consumer use.

ISO 18415 test method is applicable to both water-miscible and water-immiscible cosmetic products.

ISO 18415 test takes 1 week to complete.

At Microbe Investigations, we test for ISO 18415 using the following microbial strains:
Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P) and Candida Albicans (MTCC 3017).

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