BS EN 17122: 2019

Quantitative non-porous surface test for evaluating the virucidal activity of chemical disinfectants and antiseptics used in the veterinary area.

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Standard to Assess Virucidal Activity of Chemicals Intended for Disinfection

Quick understanding of the test

BS EN 17122: 2019 - Quantitative non-porous surface test for evaluating the virucidal activity of chemical disinfectants and antiseptics used in the veterinary area.

EN 17122 assesses the virucidal activity of chemical disinfectants and antiseptics intended for use in veterinary areas.

Application

Applicable to disinfectant products used in veterinary settings, such as breeding, husbandry, production, transport, and veterinary care facilities.
  • Test virus suspension is inoculated onto the carrier and dried.
  • The test surface is treated with the disinfectant sample for a specified contact time.
  • After the contact time, the test surface is transferred to a cell maintenance medium for neutralization. 
  • The virus titre recovered from the test surface is determined, and the reduction in virus titre is compared with control.

Turnaround Time

The test takes approximately 5-6 weeks to complete.
The efficacy of the disinfectant is determined by measuring the reduction in viral infectivity after treatment.

Passing criteria

A disinfectant must demonstrate at least a 3-log reduction in viral infectivity to meet the standard requirements.

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Abstract

EN 17122 lays out a surface test method for assessing the virucidal activity of a given chemical disinfectant or antiseptic intended to be used in veterinary areas.

EN 17122: 2019 is a phase-2 step-2 test for any antiviral disinfectant testing intended to be used in the following area and/or situation in a veterinary setting –

  • breeding, husbandry, production,
  • veterinary care facilities,
  • transport, 
  • and disposal of all animals except when in the food chain.  

The test parameters have been formulated to closely mimic the practical application condition for the disinfectant. 

EN 17122 Test Conditions & Requirements

The parameters specified by EN 17122 standard are as follows:

Test conditions

 

Test organism (viral strains)

Porcine Parvovirus (Full virucidal activity)

Feline coronavirus (Activity against enveloped viruses)

Contact Time (Minutes)

Minimum: 1  to Maximum: 120 

Other Contact times indicated by standards (Minutes)

5, 15, 30, and 60

Test Temperature (mandatory)

10 ยฐC

Additional Temperature Indicated by Standards

4, 20, and 40 ยฐC 

Interfering substance 

Low level 0.3 g/l Bovine Albumin 

High Level 3.0 g/l Bovine Albumin

Passing criteria (Log reduction)

The product must show at least 3 log reduction in infectivity

EN 17122 Test Method

  • A virus suspension with the interfering substances is inoculated onto a test surface and dried.
  • The surface with the dried virus film is then covered with the disinfectant sample (test sample) or hard water (control sample) and kept at a specific temperature for a defined period (contact time).
  • After the contact time, the test surfaces are transferred to a cell maintenance medium to neutralize the action of the disinfectant and recover the residual virus.
  • The titer of the virus recovered from the test surface is then determined by serially diluting the recovered virus and plating it on a permissive host cell line for a defined period of time.  

Importance of EN 17122 Test

EN 17122 guideline helps in evaluating the efficacy of disinfectant/antiseptic products in preventing the transmission and spread of any viral disease in the veterinary area. The disinfectant sample passing the EN 17122 test will help to maintain a safe and healthy animal environment. EN 17122 also serves as the standard testing guideline for the registration and selling of the disinfectant sample in the European zone and some additional countries.

Conclusion

At MIS, all the antiviral, antibacterial, and antifungal testing services follow international standards and guidelines. With the help of our qualified staff and certified advanced laboratory set-up, we ensure to provide precise, reliable, and expeditious services to our clients. 

Contact us here to learn more about testing your disinfectant sample using EN 17122 guidelines, turnaround time, and any other related queries. 

Frequently Asked Questions

DR. Martinoz Scholtz

MIS Labs
1. What is EN 17122 test?

EN 17122 is a phase-2 step-2 test that specifies a surface test method for evaluating the virucidal activity of chemical disinfectants and antiseptics used in the veterinary area.

2. What are the Products that can be tested with the EN 17122 test?

EN 17122 test applies to disinfectant/antiseptic products intended for disinfecting without mechanical action on non-porous surfaces in the veterinary area.

3. What is the Turnaround Time for EN 17122 test?

EN 17122 test takes 5-6 weeks to complete.

4. Which viral strains can be used to test for EN 17122?

At Microbe Investigations, we test for EN 17122 using the following microbial strains:  Porcine Parvovirus for a full virucidal activity test and Feline coronavirus to test activity against enveloped viruses.

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