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ASTM G22 : 1996
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Quick understanding of the test
ASTM G22 : 1996 - Standard method for determining resistance of plastic to bacteria
Mandatory test strains
- Pseudomonas aeruginosa (ATCC 15442)
- Procedure A: Test samples are placed on nutrient-salts agar plates inoculated with bacteria and incubated for 21 days, after which bacterial growth inhibition is examined.
- Procedure B: Test samples are sandwiched between pre-inoculated agar layers for full contact and incubated similarly, followed by growth inhibition assessment.
- Assesses durability and suitability of plastics in microbial-prone environments.
- Identifies microbial susceptibility in materials caused by additives like plasticizers or stabilizers.
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Passing criteria
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Abstract
Importance of ASTM G22 Test
Antimicrobial plastic materials have a range of benefits, such as providing antimicrobial protection, infection prevention, and preserving the durability and life span of material.
Thereby, antimicrobial testing is a crucial step to follow during manufacturing of antimicrobial plastic products to ensure the resilience of materials against harmful microorganisms before final applications.
ASTM G22 helps to determine if antimicrobial plastic material is capable of killing or hindering the growth of microorganisms under specific conditions.
Plastic materials that can be tested using ASTM G22
- fabricated articles,
- tubes,
- rods,
- Sheets,
- coatings,
- and film materials.
ASTM G22 test method
ASTM G22 standard comprises two procedures, A & B for determining the efficacy of antibacterial-treated plastics. Pseudomonas aeruginosa (ATCC 15442) is a mandatory microorganism for performing the test.
Procedure A
Test sample is placed on the surface of nutrient-salts agar plates that have already been inoculated with test microorganisms. Plates are examined for bacterial growth inhibition after 21 days of incubation.
Procedure B
Herein, complete contact of the test sample and bacterial inoculum is achieved by placing the test sample sandwiched between the nutrient-salts agar pre-inoculated with test bacteria. Similar to procedure A, plates are examined for bacterial growth inhibition after 21 days of incubation.
Conclusion
Here at MIS, we develop and optimize test protocols for ASTM G22 which comply with international standards. Test analysis is carried out in advanced laboratory setups by our microbiologists who have hands-on work experience and are well-versed with the techniques of data analysis and interpretation.
MIS also offers Antifungal Resistance Test – ASTM G21 for plastic and synthetic polymeric materials.
To request a quote on ASTM G22 and more information about our testing capacities, contact our experts here.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM G22 standard specifies a qualitative test method for determining the antibacterial resistance of additives impregnated in plastic materials.
ASTM G22 test is applicable to plastic materials available in different product forms such as
fabricated articles, tubes, rods, sheets, coatings, and film materials.
ASTM G22 test takes 4-5 weeks to complete.
At Microbe Investigations, we test for ASTM G22 using the Pseudomonas aeruginosa (ATCC 15442). Additional strains can be added as per customer request.
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