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ASTM G22: 2023
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ASTM G22 : 1996 - Standard method for determining resistance of plastic to bacteria
ASTM G22 test determines the resistance of plastic materials impregnated with antibacterial agents to bacterial growth under specific conditions.
Mandatory test strains
- Pseudomonas aeruginosa (ATCC 15442)
- Procedure A: Test samples are placed on nutrient-salts agar plates inoculated with bacteria and incubated for 21 days, after which bacterial growth inhibition is examined.
- Procedure B: Test samples are sandwiched between pre-inoculated agar layers for full contact and incubated similarly, followed by growth inhibition assessment.
- Assesses durability and suitability of plastics in microbial-prone environments.
- Identifies microbial susceptibility in materials caused by additives like plasticizers or stabilizers.
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Passing criteria
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Abstract
ASTM G22 test method is designed to determine the resistance of plastic materials impregnated with antibacterial agents. It evaluates the susceptibility of plastic materials to bacterial attack under conditions favorable for microbial growth, such as high temperature and humidity.
Resins components in plastics are inherently resistant to bacterial colonization, as they not serve carbon source for bacterial growth. It is generally the other components, such as plasticizers, lubricants, stabilizers, and colorants which increase the susceptibility for bacterial attack on plastic materials.
This method is critical for applications where plastics are exposed to demanding environmental conditions.
Importance of ASTM G22 Test
Antimicrobial plastic materials have a range of benefits, such as providing antimicrobial protection, infection prevention, and preserving the durability and life span of material.
Thereby, antimicrobial testing is a crucial step to follow during manufacturing of antimicrobial plastic products to ensure the resilience of materials against harmful microorganisms before final applications.
ASTM G22 helps to determine if antimicrobial plastic material is capable of killing or hindering the growth of microorganisms under specific conditions.
Plastic materials that can be tested using ASTM G22
- fabricated articles,
- tubes,
- rods,
- Sheets,
- coatings,
- and film materials.
ASTM G22 test method
ASTM G22 standard comprises two procedures, A & B for determining the efficacy of antibacterial-treated plastics. Pseudomonas aeruginosa (ATCC 15442) is a mandatory microorganism for performing the test.
Procedure A
Test sample is placed on the surface of nutrient-salts agar plates that have already been inoculated with test microorganisms. Plates are examined for bacterial growth inhibition after 21 days of incubation.
Procedure B
Herein, complete contact of the test sample and bacterial inoculum is achieved by placing the test sample sandwiched between the nutrient-salts agar pre-inoculated with test bacteria. Similar to procedure A, plates are examined for bacterial growth inhibition after 21 days of incubation.
Conclusion
Here at MIS, we offer precise and compliant ASTM G22 testing services to validate the antibacterial resistance of your plastics products. Test analysis is carried out in advanced laboratory setups by our microbiologists who have hands-on work experience and are well-versed with the techniques of data analysis and interpretation.
MIS also offers Antifungal Resistance Test – ASTM G21 for plastic and synthetic polymeric materials.
For a quote on ASTM G22 testing or to learn more about our capabilities, contact our experts today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM G22 standard specifies a qualitative test method for determining the antibacterial resistance of additives impregnated in plastic materials.
ASTM G22 test is applicable to plastic materials available in different product forms such as
fabricated articles, tubes, rods, sheets, coatings, and film materials.
ASTM G22 test takes 4-5 weeks to complete.
At Microbe Investigations Switzerland, we test for ASTM G22 using the Pseudomonas aeruginosa (ATCC 15442). Additional strains can be added as per customer request.
Plastic additives enhance bacterial resistance by inhibiting microbial growth and preventing surface degradation.
Yes, ASTM G22 can be used for quality control in manufacturing.
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