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ASTM E2799-12 : Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay
ASTM E2799 is a standardized method to check efficacy of disinfectants against Pseudomonas aeruginosa Biofilm using MBEC assay.
Bacterial biofilms are clusters of bacteria that are either attached to each other or to a surface to form a complex structure. These biofilms exhibit different gene expression and regulation in comparison to individual microorganisms floating freely in water (planktonic cells) even if they have the same genetic makeup.
Biofilm growth reactors are designed to produce biofilms with particular characteristics. The objective of biofilm research and efficacy testing is to select the growth reactor that produces the most suitable biofilm for the specific study.
ASTM E2799 Test Method
- The MBEC assay device (tester device) consists of a plastic lid with 96 pegs and a receiver plate with 96 individual wells that can hold a maximum 200-µL working volume.
- Biofilm is established on the pegs under batch conditions when there is no flow of nutrients into or out of an individual well with gentle mixing.
- The established biofilm is transferred to a new receiver plate for disinfectant efficacy testing.
- At the end of 24 h of growth, the pegs containing the biofilm are rinsed to eliminate planktonic cells.
- In the next step, the peg lid is placed in a receiver plate and the wells in the receiver plate are filled with appropriate sterility, growth, and neutralizer controls and disinfectant samples.
- After a specified contact time, samples from each well are diluted and plated to enumerate viable cells.
- Disinfectant efficacy is expressed as a log reduction of viable cells when biofilm density is recorded as log colony forming units per surface area.
Importance of ASTM E2799 Test
Biofilm-producing strains of Pseudomonas aeruginosa have become a concerning cause for persistent and difficult-to-treat infections in hospitalized patients. Biofilm helps bacteria to become resilient to survive under extreme conditions, hence,making their inactivation difficult by disinfectants.
The implementation of disinfectants with the ability to combat Pseudomonas aeruginosa biofilms provides a more targeted approach to disinfection, especially in situations where bacterial biofilms are of concern. To evaluate the efficacy of such disinfectants, ASTM International has developed a standard test method known as ASTM E2799, which employs the MBEC Assay to assess Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm.
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FAQs
ASTM E2799 specifies the use of biofilm assay (MBEC) to grow and evaluate the susceptibility of Pseudomonas aeruginosa biofilm to disinfectant samples.
ASTM E2799 testing is applicable to disinfectants claiming efficacy against Pseudomonas aeruginosa Biofilm.
ASTM E2799 test takes 4-5 weeks to complete.
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