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AATCC 100
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Determination of Antibacterial Activity of Textile Products
Quick understanding of the test
AATCC 100 - Test Method for Antibacterial Finishes on Textile Materials
- Staphylococcus aureus (ATCC 6538P)
- Klebsiella pneumoniae (ATCC 4352)
Methodology
- The test is performed on both treated (test) and untreated (control) fabric samples.
- Samples are inoculated with 1.0 ± 0.1 mL bacterial suspension to allow easy enumeration at 0 contact time.
- A neutralizing solution is added to half the samples at 0 contact time; the remaining samples were incubated at 37 ± 2°C for 18–24 hours.
- After incubation, neutralized samples are shaken, serially diluted, and plated to quantify viable bacteria.
- Antibacterial activity is assessed by comparing bacterial reduction between treated and untreated samples. Know more
Benefits
- Provides precise measurement of microbial reduction for accurate antimicrobial evaluation.
- Ensures compliance with quality and regulatory standards for antibacterial textile products.
Turnaround Time
Results
Passing Criteria
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Abstract
The AATCC 100 test method is a quantitative test method to determine the efficacy of antibacterial finishes applied to textile materials. Fabrics treated with antibacterial finishes offer protection to the users from harmful bacteria meanwhile reducing the risk of transmission of diseases.
AATCC 100 test method provides the evaluation of the bactericidal (bacterial killing) and bacteriostatic (inhibition of bacteria growth) activity of fabric material over a contact period of 24 hours against the test bacteria. In this method, microbial concentrations are standardized, and bacteria are provided with nutrients throughout the incubation period. If the test fabrics do not have strong antimicrobial potential, then this will allow bacteria to flourish and grow.
Similar to The AATCC 100 test, JIS L 1902 is a Japanese Industrial Standard test method to evaluate the antibacterial activity of textile products.
Organisms typically used in this method: Staphylococcus aureus (ATCC 6538P), Klebsiella pneumoniae (ATCC 4352), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 15442), Enterococcus hirae (ATCC 10541), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708) , Candida Albicans (MTCC 3017).
Products that can be tested with AATCC 100 test
The AATCC 100 is a quantitative test method used to determine the antibacterial effectiveness of a textile or porous material, such as paper filter media and tissues.
AATCC 100 test method
- AATCC 100 test is performed with both the antibacterial treated (test) and non-treated (control) samples of the fabric material.
- The amount of inoculum used for this test is 1.0 ± 0.1 mL. This ensures the easy enumeration of bacteria at “o” contact time for control.
- After inoculation (0 contact time), a neutralizing solution is added to half of the samples to suppress the bactericidal action and to maintain the right pH balance. And remaining samples are incubated at 37 °C ± 2 °C for 18 h to 24 h.
- After incubation, neutralizing solution is added to the samples. Components are shaken either by using hands or a vortex to ensure proper mixing.
- Serial dilutions of neutralizing solution are made and plated to enumerate the remaining bacteria.
- Antibacterial activity is determined by comparing the reduction in viable count of bacteria on the treated sample with the untreated sample.
- This procedure tests both bacteriostatic activity (inhibition of multiplication) and bactericidal activity (killing of bacteria) of the antimicrobial test agent. The hydrophobic characteristic of fabrics can affect the antimicrobial activity test results obtained by the AATCC 100 test method.
Frequently Asked Questions
DR. Martinoz Scholtz
The AATCC 100 standard test method is used to quantitatively determine the efficacy of antibacterial finishes applied to textile materials.
The AATCC 100 is a quantitative test method used to determine the antibacterial effectiveness of all textile products such as cloth, wadding, thread, bedclothes, home furnishings, and miscellaneous goods. This test method applies to all textile products such as cloth, wadding, thread, bedclothes, home furnishings, and miscellaneous goods.
The AATCC 100 test takes 3 to 4 days to complete.
At Microbe Investigations, we test for the AATCC 100 using the following bacteria:
Staphylococcus aureus (ATCC 6538P), Klebsiella pneumoniae (ATCC 4352), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 15442), Enterococcus hirae (ATCC 10541), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.
We are a global testing service provider in the sphere of bacteriology, virology, entomology, and odor testing. We use advanced testing techniques to run our testing procedures efficiently. Our constant focus is to provide fast and accurate results at an affordable price.
If you are interested in finding more information about our testing services or want to get a quote for AATCC 100, contact us here.
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