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JIS Z 2801
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Quick understanding of the test
JIS Z 2801 - Measurement of Antibacterial Activity on Plastics Surfaces (Plastics)
Application
- Pseudomonas aeruginosa (ATCC 15442)
- Escherichia coli (ATCC 8739)
- Staphylococcus aureus (ATCC 6538P)
- Candida albicans (MTCC 3017)
- Test samples are inoculated with bacterial suspension and incubated.
- After incubation, neutralizing solution is added onto test samples, further followed by serial dilution, plating, and incubation.
- Viable bacteria are counted and the colony-forming units (CFU) are compared between treated and untreated samples.
Benefits
- Provides a quantitative assessment of the antibacterial performance of a material, confirming the effectiveness of antimicrobial treatments.
- Helps manufacturers comply with international standards and regulatory requirements for marketing antimicrobial plastic products.
Turnaround Time
Typically, 1 – 2 weeks
Log reduction in the viable bacterial count of the test product is compared to the control.
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Abstract
JIS Z 2801 is a Japan Industrial Standard used to evaluate the antimicrobial activity and efficacy of plastic material and other hard antimicrobial surfaces.
Many bacterial species have the ability to survive on the hard surface and remain infectious for several days. This clearly increases the possibility of transmission of bacterial infections through high contact on solid surfaces. To avoid the rapid spread of such infections, it becomes prerequisite to develop antibacterial surface applications for the things that can act as primary or common sources of infection. The JIS Z2801 test method is used to assess the antibacterial effectiveness of materials such as plastics, ceramics, and other non-porous materials.
JIS Z 2801 and ISO 22196 are two identical and widely used test standards that determine the antibacterial activity of solid surfaces that are treated with antimicrobial agents. At MIS, we perform these tests as per customer’s specifications for contact period, test organisms, and other optimal conditions.
Scope of products for testing
This standard applies to:
- antimicrobial plastic products,
- Metal surfaces
- Ceramic products and
- Other hard non-porous surfaces.
The standard excludes testing of textile products and the photocatalyst products.
Mandatory test strains
- Escherichia coli (ATCC 8739)
- Staphylococcus aureus (ATCC 6538P)
Additional test strains available at MIS
- Pseudomonas aeruginosa (ATCC 15442),
- Staphylococcus aureus (ATCC 6538P),
- Enterococcus hirae (ATCC 10541),
- Klebsiella pneumoniae (ATCC 4352),
- MRSA (ATCC 33591),
- Salmonella enterica (ATCC 10708),
- Candida albicans (MTCC 3017).
JIS Z 2801 - Test procedure
- Test bacterial inoculum is placed on the testing samples (treated and untreated) and is covered with a piece of film (size of 40 mm ร 40 mm) to prevent the spread of the inoculum from the edges.
- These petri plates are incubated for 24 hrs under specific temperature (35ยฐC) and humidity conditions.
- For untreated samples (control) – 10 ml of SCDLP broth (used as a neutralizer) is added to Petri plates containing sample material immediately after incubation. Further, a 10-fold serial dilution is performed with the recovered neutralizing solution to enumerate the viable bacteria. TSA (Tryptic Soya Agar) is used as a bacterial growth medium and plates are incubated at 35 ยฐC for 40 to 48 hours.
- For treated samples (test) – 10 ml of SCDLP broth (used as a neutralizer) is added to Petri plates having samples after the incubation of 24 hours. Further, 10-fold serial dilution is performed and is plated on Plate Count Agar (PCA) to enumerate the viable bacteria. Plates are incubated at 35 ยฐC for 40 h to 48 hours.
- After incubation, CFU (Colony Forming Unit) is counted for plates (sample and control) containing 30 – 300 colonies.
- Bactericidal activity of the testing material is determined by the evaluation of the reduction in viable bacterial count of treated samples as compared to the control samples.
Strengths of the JIS Z 2801 Test
- Provides quantitative analysis of the products efficacy expressed in the form of log reduction/percentage reduction.
- Microbial concentrations are standardized which ensures that the results are reproducible across labs.
- Widely harmonized with ISO 22196 and accepted globally in hygiene-sensitive industries.
- Adaptable to many material types and bacterial strains.
- Supports regulatory and marketing needs by providing efficient data to substantiate antimicrobial claims.
Conclusion
At Microbe Investigations, we offer a comprehensive array of testing services in the sector of microbiology, virology, and entomology. In our testing lab setup, we have introduced all modern technology and best test practices to track all possible testing scenarios. Hence, enabling our customers to develop high-performance products used in the health care sector and day-to-day life.
Our goal is to simplify your needs with accurate and reliable results.
To request a quote on JIS Z 2801 test or to get more information, reach out to us here.
Frequently Asked Questions
DR. Martinoz Scholtz
JIS Z 2801 is a Japanese Industrial Standard used to evaluate the antimicrobial activity and efficacy of plastic material and other hard antimicrobial surfaces.
JIS Z 2801 is a Japanese standard that has been internationally adopted as ISO 22196. Both methods are technically similar.
JIS Z 2801 test method is applicable for plastic materials and other non-porous materials such as steel, ceramics and glass.
The test typically takes 1-2 weeks to complete.
At Microbe Investigations Switzerland, we test for JIS Z 2801 using the following bacterial strains: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida albicans (MTCC 3017). Additional strains can be added on customerโs request.
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