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JIS L 1902
Determination of Antibacterial Activity of Antibacterial
Finished Products (Textiles)
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Quick understanding of the test
JIS L 1902 - Determination of Antibacterial Activity of Antibacterial Finished Products (Textiles)
Application
- Staphylococcus aureus (ATCC 6538P)
- Klebsiella pneumoniae (ATCC 4352)
- Treated and untreated fabric samples are sterilized and inoculated with a bacterial suspension.
- After incubation samples are transferred to a neutralizing solution.
After neutralization, serial dilution and plating are performed to determine the viable bacteria count.
- Ensures antibacterial finished textiles meet consistent safety and efficacy standards.
- Evaluates both bacteriostatic (inhibition) and bactericidal (killing) properties.
Turnaround Time
Passing criteria
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Abstract
Different methods of testing for JIS L 1902
Absorption Method – In this evaluation method test bacteria are inoculated directly onto the samples.
Transfer Method – An evaluation method in which test bacteria are placed on an agar plate and then transferred onto the samples.
Printing Method – In this method, test bacteria are placed on a filter and printed onto samples.
Halo Method – This is a qualitative method that evaluates the antibacterial activity by the “zone of inhibition” around the treated sample. For JIS L 1902 halo method, test bacteria are inoculated into molten agar and covered by the test samples once agar gets solidified. These plates are incubated for 24- 48 hours to measure the zone of inhibition (halo).
Bacterial strains used for JIS L 1902
Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.
JIS L 1902 test procedure
- 6 test specimens of treated (antibacterial) and 6 control samples of untreated (without antibacterial agents) fabric are needed.
- All sample materials are subjected to sterilization to avoid any contamination.
- Test bacterial suspension of a known titre is directly inoculated on the sample material.
- For zero minute titre determination, (control and treated) samples are subjected to immediate wash with 20 ml of neutralizing media (SCDLP broth) after bacterial inoculation. Other remaining six specimens (3 control and 3 testing samples) are incubated at 37 °C ± 2 °C for 18 h to 24 h with the test bacterial suspension
- After an incubation period of 18-24 h, the reaction is stopped by adding 20 ml of neutralizing media Soya Casein Digest Lecithin Polysorbate Broth (SCDLP) into tubes. Then, the tubes are shaken to ensure proper mixing.
- Serial dilution and plating are done to determine titre of the remnant viable bacteria post the contact time
- The decrease in microorganism growth is compared to the control fabric.
Major advantages of JIS L 1902
JIS L 1902 test method has a fast turnaround time and is also ideal for large sample quantities.
Conclusion – Ensuring cosmetic safety with ISO 21149 testing
With the growing awareness of bacterial diseases and their hazards to mankind, there is an increased demand for antibacterial fabrics. But before these antibacterial treated clothes are put into use, standard methods are used to evaluate their antibacterial efficacy.
At Microbe Investigations, we have the facilities and resources to perform all different antimicrobial fabric tests such as JIS L 1902, AATCC 100, ISO 20743, and so on. Our research and test laboratories encompass all the essential equipments, tools, and technical experts to deliver comprehensive solutions to meet the needs of our customers.
Consult our experts for additional details on our antimicrobial fabric testing services.
Frequently Asked Questions
DR. Martinoz Scholtz
JIS L 1902 test method is used to check the antibacterial activity of all finished textile materials such as cloth, wadding, thread, bedclothes, home furnishings, and miscellaneous goods.
At Microbe Investigations, we test for the JIS L 1902 using the following viruses: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida Albicans (MTCC 3017). Additional strains can be added on request.
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