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ISO 18415: 2017
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Quick understanding of the test
ISO 18415: 2017 - Detection of specified and non-specified microorganisms in cosmetic products
Application
Mandatory test strains
- Pseudomonas aeruginosa (ATCC 15442)
- Escherichia coli (ATCC 8739)
- Staphylococcus aureus (ATCC 6538P)
- Candida albicans (MTCC 3017)
The test involves diluting cosmetic samples in enrichment broth, followed by incubation to detect microbial growth.
After incubation, an aliquot of the incubated enrichment broth is streaked onto non-selective agar and incubated.
Any present microorganisms are identified using specialized identification kits.
- Validates microbiological safety of products.
- Help manufacturers to meet regulatory safety standards for cosmetic products.
Turnaround Time
Passing criteria
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Abstract
ISO 18415 standard describes a test method to identify the presence of any specified microorganisms and anaerobic mesophilic non-specified microorganisms in cosmetic products.
ISO 18415 outlines guidelines for the microbiological risk analysis specifically for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Candida albicans.
ISO 18415 involves the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable for detecting microbial contamination. This is further followed by the isolation of microorganisms on non-selective agar media.
ISO 18415 Test Method
- To perform the test, cosmetic product samples are diluted in enrichment broth to prepare the initial suspension.
- Water-miscible products – 1 ml of product sample is diluted in 9 ml of enrichment broth.
- Water-immiscible products – The product sample is mixed with a dispersing agent (e.g., polysorbate 80) and then added to the enrichment broth.
- Filterable products – The product sample is transferred onto the membrane in a filtration apparatus. After filtration, the membrane is washed using water or diluent. This membrane is transferred into enrichment broth to make an initial suspension.
- This initial suspension is incubated at 32.5 °C for at least 20 h.
- After incubation, an aliquot of the incubated enrichment broth is streaked onto the surface of a non-selective agar medium in Petri dishes.
- Petri dishes are inverted and incubated at 32.5 °C for 48 h to 72 h.
- Identification of microorganisms is done using an identification kit. This kit provides instructions for inoculation, incubation, and reading. The final results of the identification methods are compared with the database for final analysis.
Importance of ISO 18415 Test
Microbially contaminated cosmetic products can cause skin allergies, irritations, and other significant health concerns to the end users. Therefore, manufacturers/ companies need to ensure their products meet the necessary microbiological safety requirements.
ISO 18415 standard stipulates the test requirements and procedures to identify potential microbial contaminants in cosmetic products. This method plays a vital role in validating the safety of cosmetic products for the well-being of customers.
Conclusion
Need assistance with ISO 18415 testing of your cosmetic products? MIS experts are here to help you.
We offer a comprehensive range of customizable solutions for detecting microorganisms in cosmetic and personal care products. Our cosmetic testing services encompass the screening of products at various stages of development, including ingredients, semi-manufactured products, and final products.
Additionally, we also test the antimicrobial efficacy of preservatives used in cosmetic products.
In our state-of-the-art testing facilities, we are dedicated to delivering test results of the highest quality and accuracy.
Furthermore, customers can also send their product samples for ISO 18416 testing.To get a quote on the ISO 18415 test or any other queries related to our cosmetic testing services, reach out to our experts here.
Frequently Asked Questions
DR. Martinoz Scholtz
ISO 18415 is a standard test method for the identification of both specified and non-specified microbial contaminants in cosmetic products. The objective of the test is to ensure that cosmetic products are safe for consumer use.
ISO 18415 test method is applicable to both water-miscible and water-immiscible cosmetic products.
ISO 18415 test takes 1 week to complete.
At Microbe Investigations, we test for ISO 18415 using the following microbial strains:
Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P) and Candida albicans (MTCC 3017).
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