ISO 18415

ISO 18415 Test Method

• To perform the test, cosmetic product samples are diluted in enrichment broth to prepare initial suspension.
  • 1. Water-miscible products – 1 ml of product sample is diluted in 9 ml of enrichment broth.
    2. Water-immiscible products –  Product sample is mixed with a dispersing agent (e.g. polysorbate 80) and then added to the enrichment  broth.
    3. Filterable products – Product sample is transferred onto the membrane in a filtration apparatus. After filtration, the membrane is washed using water or diluent. This membrane is transferred into enrichment broth to make initial suspension.
• This initial suspension is incubated at 32.5 °C for at least 20 h.
• After incubation, an aliquot of the incubated enrichment broth is streaked onto the surface of non-selective agar medium in petri dishes.
• Petri dishes are inverted and  incubated at 32.5 °C for 48 h to 72 h. 
• Identification of microorganisms is done using an identification kit. This kit provides instructions for inoculation, incubation, and reading. Final results of the identification methods are compared with the database for final analysis.